No abstract

We report on a voltammetric sensor for the detection of total cholesterol. The sensor was fabricated by co-immobilization of two enzymes: cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) on porous graphite. The electrochemical behavior of the sensor was studied with the use of linear sweep voltammetry. It has been shown that the sensor has high stability and high sensitivity (16 muA mM{-1} cm{-2}). The biosensor exhibited a wide linear range up to 300 mol/dm3 in a condition close to physiological (pH=6.86). Besides, the interferences of some key analytes containing in the blood were studied. As a matter of fact, making a fabricated sensor is rather promising for using in clinical practice

The treatment of some inborn metabolism errors requires cholesterol substitution therapy. Cholesterol plays a vital role in the human body. Therefore, the majority of cholesterol determination techniques are targeted to blood and blood serum. Nevertheless, cholesterol determination in food is important as well. In this paper, cholesterol determination using differential pulse voltammetry (DPV) in dairy products (e.g., milk, clotted cream, yogurt, butter, etc.) is reported with a novel nonenzymatic sensor based on diphosphonic acid of 1,4-diacetylglycoluril (DPADGU) as an electrode surface modifier. Stable anodic response was obtained from cholesterol on the modified carbon-based electrode. The sensor has high stability, sensitivity (20 μA mol L cm), and a wide linear range from 1 up to 200 μM. The LOD and LOQ values are 1.5 and 5.1 μM, respectively. The developed methods were successfully applied to the above mentioned dairy products. Graphical abstract ᅟ.

In this paper for investigation of electrochemical properties of nitrogenous bases by voltammetry xanthine (Xa), adenine (A) and thymine (T) with constant-current potential sweep with differentiation were used. The electrochemical behavior of Xa, A and T on the surface of a glassy carbon electrode were investigated. The conditions of registration of their joint detecting in the solution were defined. It is demonstrated that the oxidation peak currents of Xa, A and T increased linearly with their concentration in the range of 4.0 10{-8} - 1 10{-4} mol/dm{3} for Xa, 3.0 10{-7} – 1.0 10{-4} mol/dm{3} for A, and 1.0 10{-5}– 1.1 10{-3} mol/dm{3} for T with correlation coefficients of 0.996, 0.996 and 0.999, respectively

No abstract