Non-enzymatic electrochemical determination of creatinine using a novel screen-printed microcell

Fava, Elson Luiz; Prado, Thiago Martimiano do; Garcia-Filho, Amauri; Silva, Tiago; Cincotto, Fernando Henrique; Moraes, Fernando C.; Faria, Ronaldo C.; Fatibello‐Filho, Orlando

doi:10.1016/j.talanta.2019.120277

Cited by 46 publications

(22 citation statements)

References 26 publications

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“…Therefore, no significant changes in the analytical signal of CNN were observed. In addition, the results are in agreement with those presented by Fava et al 2020 [ 40 ].…”

Section: Resultssupporting

confidence: 93%

“…In this scenario, electrochemical sensors are presented as attractive analytical devices because they combine high sensitivity, simplicity of operation, and low cost [ 38 ]. In the literature, various types of enzymatic [ 39 ] and non-enzymatic [ 40 , 41 ] sensors are reported for the determination of CNN with different electrochemical techniques [ 42 , 43 , 44 ]. Although selective, enzyme biosensors have some limitations when compared to non-enzymatic electrodes.…”

Section: Introductionmentioning

confidence: 99%

“…In this context, the high cost and denaturation, time-consuming preparation, and lack of stability can be highlighted as negative points that can be contoured when non-enzymatic sensors are applied. Thus, the development of non-enzymatic sensors for the detection of CNN is a highly viable and advantageous option [ 40 , 45 , 46 ]. However, works that explore the use of miniaturized and portable devices for CNN determination are still lacking, and the use of 3D-printed devices for the detection of CNN has not been reported in the literature until now.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes

et al. 2022

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A low-cost and disposable graphene polylactic (G-PLA) 3D-printed electrode modified with gold particles (AuPs) was explored to detect the cDNA of SARS-CoV-2 and creatinine, a potential biomarker for COVID-19. For that, a simple, non-enzymatic electrochemical sensor, based on a Au-modified G-PLA platform was applied. The AuPs deposited on the electrode were involved in a complexation reaction with creatinine, resulting in a decrease in the analytical response, and thus providing a fast and simple electroanalytical device. Physicochemical characterizations were performed by SEM, EIS, FTIR, and cyclic voltammetry. Square wave voltammetry was employed for the creatinine detection, and the sensor presented a linear response with a detection limit of 0.016 mmol L−1. Finally, a biosensor for the detection of SARS-CoV-2 was developed based on the immobilization of a capture sequence of the viral cDNA upon the Au-modified 3D-printed electrode. The concentration, immobilization time, and hybridization time were evaluated in presence of the DNA target, resulting in a biosensor with rapid and low-cost analysis, capable of sensing the cDNA of the virus with a good limit of detection (0.30 µmol L−1), and high sensitivity (0.583 µA µmol−1 L). Reproducible results were obtained (RSD = 1.14%, n = 3), attesting to the potentiality of 3D-printed platforms for the production of biosensors.

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“…Therefore, no significant changes in the analytical signal of CNN were observed. In addition, the results are in agreement with those presented by Fava et al 2020 [ 40 ].…”

Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes

et al. 2022

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“…PANi [22] Enzymatic Amperometric 50 50-1000 Poly venyle chloride [23] Enzymatic Potentiometric 44-88 88-4400 Paper absorbed Fe (III) ions [24] Microfluidic cartridge [25] Non To examine the durability of the ZIF-8 NPs/PEDOT:PSS/ITO based creatinine sensor, the durability test was performed for 21 days, as shown in Figure 8. The EIS measurement of the creatinine sensor was recorded in 1 M creatinine containing electrolyte for day 0, day 2, day 5, day 7, day 14, and day 21.…”

Section: Sensing Materials Methods Of Detection Measurement Technique...mentioning

confidence: 99%

ZIF-8 Nanoparticles Based Electrochemical Sensor for Non-Enzymatic Creatinine Detection

et al. 2022

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There is a consistent demand for developing highly sensitive, stable, cost-effective, and easy-to-fabricate creatinine sensors as creatinine is a reliable indicator of kidney and muscle-related disorders. Herein, we reported a highly sensitive and selective non-enzymatic electrochemical creatinine sensor via modifying poly(3,4 ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) coated indium tin oxide (ITO) substrate by zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs). The topography, crystallinity, and composition of the sensing electrode were characterized by field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The peroxidase-like activity of ZIF-8 nanoparticles enabled it to detect creatinine forming a zinc-creatinine composite. The electrochemical behavior and sensing performance were evaluated by amperometric and impedimetric analysis. The sensor obtained a sufficiently low limit of detection (LOD) of 30 µM in a clinically acceptable linear range (0.05 mM–2.5 mM). The interference study demonstrated high selectivity of the sensor for creatinine concerning other similar biomolecules. The sensing performance of the creatinine sensor was verified in the actual human serum, which showed excellent recovery rates. Hence, the magnificent performance of ZIF-8 based non-enzymatic creatinine sensor validated it as a responsible entity for other complicated renal markers detection.

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“…Therefore, the development of simple and effective nonenzymatic techniques similar to the clinical diagnosis kits devices for the real-time monitoring of glucose is an important goal for point of care (POC) measurement of creatinine levels. A summary of prior work conducted for nonenzymatic electrochemical detection of creatinine and the gaps in these techniques are shown in Table S1. ,− As mentioned in Table S1, the existing literature reveals that although each exploration involves a unique creatinine detection method, significant gaps still exist. They are as follows: (i) the current responses of the sensors are low in the microamps range and (ii) the lowest and highest creatinine levels in human blood so far reported are out of detection limits.…”

Section: Introductionmentioning

confidence: 99%

Facile Detection of Blood Creatinine Using Binary Copper–Iron Oxide and rGO-Based Nanocomposite on 3D Printed Ag-Electrode under POC Settings

Singh

Mandal

Roy

et al. 2021

ACS Biomater. Sci. Eng.

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Metal nanoparticles have been helpful in creatinine sensing technology under point-of-care (POC) settings because of their excellent electrocatalyst properties. However, the behavior of monometallic nanoparticles as electrochemical creatinine sensors showed limitations concerning the current density in the mA/cm2 range and wide detection window, which are essential parameters for the development of a sensor for POC applications. Herein, we report a new sensor, a reduced graphene oxide stabilized binary copper–iron oxide-based nanocomposite on a 3D printed Ag-electrode (Fe–Cu–rGO@Ag) for detecting a wide range of blood creatinine (0.01 to 1000 μM; detection limit 10 nM) in an electrochemical chip with a current density ranging between 0.185 and 1.371 mA/cm2 and sensitivity limit of 1.1 μA μM–1 cm–2 at physiological pH. Interference studies confirmed that the sensor exhibited no interference from analytes like uric acid, urea, dopamine, and glutathione. The sensor response was also evaluated to detect creatinine in human blood samples with high accuracy in less than a minute. The sensing mechanism suggested that the synergistic effects of Cu and iron oxide nanoparticles played an essential role in the efficient sensing where Fe atoms act as active sites for creatinine oxidation through the secondary amine nitrogen, and Cu nanoparticles acted as an excellent electron-transfer mediator through rGO. The rapid sensor fabrication procedure, mA/cm2 peak current density, a wide range of detection limits, low contact resistance including high selectivity, excellent linear response (R 2 = 0.991), and reusability ensured the application of advanced electrochemical sensor toward the POC creatinine detection.

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Non-enzymatic electrochemical determination of creatinine using a novel screen-printed microcell

Cited by 46 publications

References 26 publications

Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes

Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes

ZIF-8 Nanoparticles Based Electrochemical Sensor for Non-Enzymatic Creatinine Detection

Facile Detection of Blood Creatinine Using Binary Copper–Iron Oxide and rGO-Based Nanocomposite on 3D Printed Ag-Electrode under POC Settings

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