Non-equilibrium acid-base status in C. productus: Role of exoskeletal carbonate buffers

deFur, Peter L.; Wilkes, P. R. H.; McMahon, Brian R.

doi:10.1016/0034-5687(80)90118-8

Cited by 74 publications

(13 citation statements)

References 21 publications

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“…The literature often links elevated Ca level with emersion-induced acidosis (deFur et al 1980;Burnett 1988). Naturally, this distinction is little help when discriminating emersed survivors.…”

Section: Discussionmentioning

confidence: 99%

Predicting survival of western rock lobstersPanulirus cygnususing discriminant analysis of haemolymph parameters taken immediately following simulated handling treatments

Paterson¹,

Spanoghe

Davidson

et al. 2005

New Zealand Journal of Marine and Freshwater Research

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Instances of morbidity amongst rock lobsters (Panulirus cygnus) arriving at factories in Western Australia (WA) have been attributed to stress during post-harvest handling. This study used discriminant analysis to determine whether physiological correlates of stress following a period of simulated post-harvest handling had any validity as predictors of future rejection or morbidity of western rock lobsters. Groups of 230 western rock lobsters were stored for 6 h in five environments (submerged/flowing sea water, submerged/ recirculating sea water, humid air, flowing seawater spray, and recirculated seawater spray). The experiment was conducted in late spring (ambient sea water 22°C), and repeated again in early autumn (ambient sea water 26°C). After 6 h treatment, each lobster was graded for acceptability for live export, numbered, and its haemolymph was sampled. The samples were analysed for a number of physiological and health status parameters. The lobsters were then stored for a week in tanks in the live lobster factory to record mortality. The mortality of lobsters in the factory was associated with earlier deviations in haemolymph parameters as they emerged from the storage treatments. Discriminant analysis (DA) of the haemolymph assays enabled the fate of 80-90% of the lobsters to be correctly categorised within each experiment. However, functions derived from one experiment were less accurate at predicting mortality when applied to the other experiments. One of the reasons for this was the higher mortality and the more severe patho-physiological changes observed in lobsters stored in humid air or sprays at the higher temperature. The analysis identified lactate accumulation during emersion and associated physiological and haemocyte-related effects as a major correlate of mortality. Reducing these deviations, for example by submerged transport, is expected to ensure high levels of survival. None of the indicators tested predicted mortality with total accuracy. The simplest and most accurate means of comparing emersed treatments was to count the mortality afterwards.

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Section: Discussionmentioning

confidence: 99%

Predicting survival of western rock lobstersPanulirus cygnususing discriminant analysis of haemolymph parameters taken immediately following simulated handling treatments

Paterson¹,

Spanoghe

Davidson

et al. 2005

New Zealand Journal of Marine and Freshwater Research

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“…Elevated levels of metal ions may reduce the need to rely on anaerobic respiration by increasing the capacity for aerobic respiration, via an increase in haemocyanin-O 2 affinity. Furthermore, once anaerobic respiration has commenced, Mg 2+ and Ca 2+ may ameliorate the associated costs by buffering against the acidosis caused by the accumulation of l-lactate (DeFur et al 1980;Henry et al 1981). It is curious, however, that attackers that do not effect an eviction have lower [Ca 2+ ] HL than both successful attackers and controls (Fig.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The circulating metal ions Ca 2+ and Mg 2+ have the ability to modulate respiratory performance. Dissolution of the CaCO 3 exoskeleton can be used by crustaceans to buffer against acid-base balance disturbances, including those caused by elevated lactate, resulting in an increase in [Ca 2+ ] HL (DeFur et al 1980;Henry et al 1981;Cameron 1985). This is not restricted to the Crustacea however, and an analogous system is also present in vertebrates with a carapace, such as the painted turtle Chrysemys picta (Jackson 2000).…”

Section: Introductionmentioning

confidence: 99%

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The Role of Circulating Metal Ions During Shell Fights in the Hermit Crab Pagurus bernhardus

et al. 2008

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Fighting animals must make a series of decisions, and understanding the proximate causes behind these decisions can give insight into how they are made. For example, fights have been analysed with respect to energetic costs and endocrine changes associated with engaging. However, another mechanism for the control of demanding activity, such as fighting, is the modulation of aerobic capacity by divalent metal ions. Here we examine post‐fight haemolymph concentrations of magnesium (Mg2+) and calcium (Ca2+) ions in the common European hermit crab Pagurus bernhardus. Hermit crabs fight over the ownership of gastropod shells, where they adopt two very different roles during the encounter: attacker and defender. Despite the two roles performing different activities, we found that Mg2+ and Ca2+ affected them similarly, with concentrations of these ions being highest in successful individuals. Haemolymph concentrations of Mg2+ and Ca2+ were also found to increase as a result of fighting, and these elevated levels will, via allosteric interactions, increase the oxygen affinity of the respiratory pigment haemocyanin, enhancing respiratory capacity and therefore fighting ability. Furthermore, the present study revealed that seasonal changes in circulating levels, along with the ability of competitors to respond to them, may ultimately influence an individual’s success in aggressive interactions.

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“…DeFur et al (1980) provided evidence that calcium carbonate, mobilised from possibly the carapace or gut, can act as an endogenous source of base during emersion of Cancer productus, and a similar process has been suggested to occur in Cardisoma carnifex during exercise recovery in air. Haemolymph [Ca 2+ ] of Cyclograpsus did not change during exercise recovery in water, so that if calcium carbonate contributes to the drop in AH+ m , simultaneous regulation of haemolymph calcium must occur.…”

Section: Metabolic Recoverymentioning

confidence: 95%

Acid‐base disturbances following exercise in a high‐shore crab,Cyclograpsus lavauxi

Waldron

Taylor

Forster

1986

New Zealand Journal of Marine and Freshwater Research

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Cyclograpsus lavauxi, a high-shore crab, suffered a marked acidosis after 10 minutes of moderate exercise in air because of increases in haemolymph lactate concentration and P CO2 . During recovery in sea water, the levels of lactate and P CO2 decreased rapidly and resting pH was restored within 2 h. In air, lactate was also metabolised rapidly, but P CO2 levels declined more slowly than in water and were partly the reason for the continued depression of haemolymph pH at 24 h. There were discrepancies between the concentrations of lactate anions and metabolic protons in the haemolymph during recovery. Protons were removed more rapidly than lactate anions in sea water, which suggests that branchial excretion of acid, and/or uptake of base, was involved in acid-base regulation. The metabolic acid load persisted throughout recovery in air and was, despite possible buffering by mobilisation of CaCO 3 , mainly responsible for the continued depression of haemolymph pH. It is concluded that sea water is necessary for the efficient restoration of haemolymph acid-base status after exercise in this species.

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Non-equilibrium acid-base status in C. productus: Role of exoskeletal carbonate buffers

Cited by 74 publications

References 21 publications

Predicting survival of western rock lobstersPanulirus cygnususing discriminant analysis of haemolymph parameters taken immediately following simulated handling treatments

Predicting survival of western rock lobstersPanulirus cygnususing discriminant analysis of haemolymph parameters taken immediately following simulated handling treatments

The Role of Circulating Metal Ions During Shell Fights in the Hermit Crab Pagurus bernhardus

Acid‐base disturbances following exercise in a high‐shore crab,Cyclograpsus lavauxi

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