Non‐frozen preservation of umbilical cord blood

Koenigbauer, Ulrike; Burger, Scott R.; McCullough, Jeffrey

doi:10.1046/j.1537-2995.2002.00259.x

Cited by 4 publications

(6 citation statements)

References 2 publications

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“…On the contrary and consistent with our data, other publications reported no differences between refrigerated storage and storage at room temperature [23,24]. The causes for the apparent discrepancies are not evident but may be due to vague definition of temperatures and conditions on one hand and to different cell analytical methods and the heterogeneity of CB itself on the other hand.…”

Section: Discussionsupporting

confidence: 80%

Evaluation of Quality Parameters for Cord Blood Donations

Salge-Bartels

Huber²,

Kleiner³

et al. 2009

Transfus Med Hemother

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Background: Umbilical cord blood (CB) is widely used for hematopoietic stem cell transplantation and holds promise for the development of innovative medicinal products. In order to find out whether the conditions for collection and storage before processing might have an impact on the quality of CB preparations, viability and the clonogenic potential were assessed. Methods: CB was collected under field conditions. Flow cytometry was used to determine leukocytes, CD34/CD45+ cells, viability, and nucleated red blood cells (NRBC). Clonogenic activity was determined using isolated mononuclear cells (MNC). Results: Neither plasma citrate concentrations nor storage temperature (within 24 h) affected cell viability or colony formation. After storage for 49–80 h, leukocyte viability declined by about 16% compared to CB stored up to 24 h. In contrast, the clonogenic activity and CD34/CD45+ cell content were not affected. A higher gestational age was associated with a lower yield of clonogenic activity compared to midterm deliveries. NRBC varied widely (median 7.3%; range 0.63–17.3%) without relation to gestational age or colony formation. There was a close correlation between the percentage of viable CD34/CD45+ cells and colony formation (r = 0.77 for CFU-GM; r = 0.75 for CFU-C). Conclusions: The content of viable CD34/CD45+ cells represents the clonogenic activity of CB preparations. Therefore, determination of viable CD34/CD45+ cells should be generally performed as a routine quality control assay.

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Section: Discussionsupporting

confidence: 80%

Evaluation of Quality Parameters for Cord Blood Donations

Salge-Bartels

Huber²,

Kleiner³

et al. 2009

Transfus Med Hemother

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“…Viability and CD34+ cells are used as quality measures, and should be included in the routine processing of the units [2,4,6]. Other methods, such as counting of mononuclear cells (MNC) or colony forming cells (CFC) have also been frequently used [1][2][3][4]13], but the first one does not predict the results after transplantation as well as CD34+ [7,11,12], and the second is technically more complex and cannot be used as a routine screening for hundreds of units. Furthermore, a good correlation can be found between the measurement of CD34+ cells and the nuclear total cells in the unit [6].…”

Section: Discussionmentioning

confidence: 99%

“…The UCB banks must be dynamic, standardized and have high-quality in their cryopreserved units which could be influence by the running time before cryopreservation. [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Influence on Time to Cryopreservation in Umbilical Cord Blood Units

Prat-Arrojo¹,

Ponce-Verdugo²,

Hernández-Lamas³

et al. 2011

IJCM

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“…The controversial results show that UCB units must be stored before processing at 4°C [4][5][6][7] or at room temperature [8][9][10]. Other authors consider that there are no differences reflected in the quality of transplant units depending on the storage temperature [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…Although most authors recommend that processing and cryopreservation must be performed as soon as possible after umbilical cord blood collection to maintain the characteristics of immature cells, other authors [10] suggest that a storage time for 48 h should be preferred to 24 h for maintenance of progenitor cells with high capacity of proliferation, transmigration, with a low rate of apoptosis. In the issue, the maximum storage time for umbilical cord blood from collection to processing and cryopreservation may be 48 or 72 h [2,12].…”

Section: Introductionmentioning

confidence: 99%