Non-genomic effect of steroids on oxytocin-stimulated intracellular mobilization of calcium and on prostaglandin F2α and E2 secretion from bovine endometrial cells

Duras, M.; Mlynarczuk, Jarosław; Kotwica, J.

doi:10.1016/j.prostaglandins.2005.02.001

Cited by 35 publications

(31 citation statements)

References 46 publications

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“…In the endometrium not exposed to embryos in vivo (isolated non-gravid uterine horn), OT-induced secretion of PGE2 in the presence of P4 during pregnancy was inhibited. The inhibitory effect of P4 on OT action has been observed in pig and cow uteri [7,8,24,25]. The current results show that the porcine embryos and their signals determine locally the response of the endometrium to P4 and to OT in the presence of P4.…”

supporting

confidence: 61%

mentioning

confidence: 99%

“…The uterine response to OT depends on expression of OTR mRNA and protein [7,[17][18][19] and on generation of the second messengers inositol triposhate and diacyloglycerol [7,18,20,21]. OT also stimulates PGF2α secretion from the bovine endometrium through a previously discussed signaling pathway [22][23][24][25].It has previously been shown that OT acts on the porcine endometrium as a regulator of secretion of both luteolytic PGF2α [3,6] and luteotrophic PGE2 [6]. Progesterone (P4) inhibits OTstimulated production of endometrial and myometrial PG via mechanisms including regulation of OTR expression, inositol phosphate accumulation and intracellular Ca 2+ response [7,19,26,27].…”

mentioning

confidence: 99%

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Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Franczak

Bogacki

2009

J. Reprod. Dev.

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Abstract. In the pig, the periimplantation period is critical for successful establishment of pregnancy. We studied the influence of embryos on oxytocin (OT) and progesterone (P4) regulated endometrial and myometrial secretion of 1) luteotrophic prostaglandin E2 (PGE2) and 2) luteolytic prostaglandin F2α and its metabolite (PGFM) on days 12-14 of pregnancy in pigs. We used unilaterally pregnant pigs created by a surgical procedure in which one uterine horn remained intact and the second horn was cut transversely so that part of the horn was detached from the uterine body. The animals were divided into two groups, inseminated gilts (days 12-14 of pregnancy, n=6) and uninseminated cyclic gilts, which were used as controls (days 12-14 of estrous cycle, n=5). Embryos developed only in the patent part of the uterus and not in the occluded horn. The abundance of OTR mRNA was increased in the endometrium and decreased in the myometrium of the gravid uterine horn in the pregnant pigs compared with the non-gravid uterine horn or either uterine horn in the cyclic pigs, indicative of a local effect of the conceptus. The presence of embryos in the uterine horn during the periimplantation period determines endometrial metabolism of PGF2α and the local response of the endometrium to OT and P4. OT stimulates PGF2α secretion and PGFM accumulation in endometrial cultures only from the non-gravid uterine horn and controls PGE2 secretion from the endometrium and myometrium in the pregnant gilts. The results indicate a more systemic affect of pregnancy on the uterine response to OT and a possibly the local effect of the conceptus in promoting progesterone's inhibition of OT-stimulated PGE2 secretion and uterine metabolism of PGF2α. Key words: Embryos, Oxytocin, Pigs, Progesterone, Prostaglandin E2, Prostaglandin F2α and its metabolite, Uterus (J. Reprod. Dev. 55: [262][263][264][265][266][267][268][269][270][271][272] 2009) rostaglandins (PG) produced in the uterus and corpus luteum are necessary for successful reproduction [1,2]. Oxytocin, acting through oxytocin receptors (OTR) in the endometrium and myometrium, is involved in the control of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) secretion in pigs [3][4][5][6][7][8]. Prostaglandin F2α is responsible for luteal regression in non-pregnant pigs [1,9], while prostaglandin E2 acts as a luteotrophic and antiluteolytic factor that promotes maintenance of the corpus luteum during early pregnancy in pigs [10,11].Alteration of PG secretion by OT is implicated in maternal recognition of pregnancy in pigs [12][13][14]. Moreover, OT stimulates myometrial secretion of PGF2α and PGE2 as well as the contractile activity of the porcine myometrium during early pregnancy [8,15,16]. The uterine response to OT depends on expression of OTR mRNA and protein [7,[17][18][19] and on generation of the second messengers inositol triposhate and diacyloglycerol [7,18,20,21]. OT also stimulates PGF2α secretion from the bovine endometrium through a previously discussed signaling pathway [22][2...

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supporting

confidence: 61%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Franczak

Bogacki

2009

J. Reprod. Dev.

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“…P 4 was assayed using RIA/IRMA kits from DSL (Webster, MA, USA) according to the manufacturer's instructions. OT, PGE-2, and PGF metabolites (PGFM) were assayed by our own RIA/EIA systems (Kotwica & Skarzynski 1993, Skarzynski et al 1999, Duras et al 2005 respectively. Determination of PGFM in medium reliably reflects PGF-2a secretion (rZ0.92; P!0.001) from cultured cells (Skarzynski et al 1999).…”

Section: Immunoassaysmentioning

confidence: 99%

Transcription factor p53 can regulate proliferation, apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH

Sirotkin¹,

Benco²,

Tandlmajerová³

et al. 2008

Reproduction

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The aim of our in vitro experiments was to examine the role of transcription factor p53 in controlling the basic functions of ovarian cells and their response to hormonal treatments. Porcine ovarian granulosa cells, transfected and non-transfected with a gene construct encoding p53, were cultured with ghrelin and FSH (all at concentrations of 0, 1, 10, or 100 ng/ml). Accumulation of p53, of apoptosisrelated (MAP3K5) and proliferation-related (cyclin B1) substances was evaluated by immunocytochemistry. The secretion of progesterone (P 4 ), oxytocin (OT), prostaglandin F (PGF), and E (PGE) was measured by RIA. Transfection with the p53 gene construct promoted accumulation of this transcription factor within cells. It also stimulated the expression of a marker of apoptosis (MAP3K5). Over-expression of p53 resulted in reduced accumulation of a marker of proliferation (cyclin B1), P 4 , and PGF secretion and increased OT and PGE secretion. Ghrelin, when added alone, did not affect p53 or P 4 , but reduced MAP3K5 and increased PGF and PGE secretion. Over-expression of p53 reversed the effect of ghrelin on OT, caused it to be inhibitory to P 4 secretion, but did not modify its action on MAP3K5, PGF, or PGE. FSH promoted the accumulation of p53, MAP3K5, and cyclin B1; these effects were unaffected by p53 transfection. These multiple effects of the p53 gene construct on luteinizing granulosa cells, cultured with and without hormones 1) demonstrate the effects of ghrelin and FSH on porcine ovarian cell apoptosis and secretory activity, 2) confirm the involvement of p53 in promoting apoptosis and inhibiting P 4 secretion in these cells, 3) provide the first evidence that p53 suppress proliferation of ovarian cells, 4) provide the first evidence that p53 is involved in the control of ovarian peptide hormone (OT) and prostaglandin (PGF and PGE) secretion, and 5) suggest that p53 can modulate, but probably not mediate, the effects of ghrelin and FSH on the ovary. Reproduction (2008) 136 611-618

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“…P 4 , PGF and OXT were assayed by our own RIA/EIA systems (Kotwica & Skarzynski 1993, Skarzynski et al 1999, Duras et al 2005. Determination of PGFM in medium reliably reflects PGF 2a secretion (rZ0.92; P!0.001) from cultured cells (Skarzynski et al 1999).…”

Section: Immunoassaysmentioning

confidence: 99%

Involvement of the transcription factor STAT1 in the regulation of porcine ovarian granulosa cell functions treated and not treated with ghrelin

Benco

Sirotkin²,

Vašíček³

et al. 2009

Reproduction

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The aim of our in vitro experiments was to study the role of the transcription factor STAT1 and the hormone ghrelin in controlling porcine ovarian function. The effects of treatment with ghrelin (0, 1, 10, 100 ng/ml), transfection-induced overexpression of transcription factor STAT1, and their combination on apoptosis (expression of apoptosis-related peptides caspase-3, BAX and anti-apoptotic peptide BCL2), proliferation (expression of proliferating cell nuclear antigene PCNA, proliferation-associated protein kinase MAPK/ERK1,2) and release of the hormones progesterone (P 4 ), prostaglandin F (PGF) and oxytocin (OXT) in cultured porcine ovarian granulosa cells was evaluated using RIA, immunocytochemistry and SDS-PAGE-western immunoblotting. It was found that ghrelin, when given alone, increased the expression of proliferation-associated PCNA and MAPK/ERK1,2, decreased the accumulation of apoptosis-related substances caspase-3, BAX, BCL2, decreased P 4 , and increased PGF and OXT release. Ghrelin tended to promote accumulation of STAT1 in both control and transfected cells, although in transfected cells ghrelin at 1 ng/ml decreased STAT1 accumulation. Transfection of porcine granulosa cells by a gene construct encoding STAT1 promoted the expression of STAT1 and apoptosis-related-BAX but the expression of BCL2 did not, and decreased the accumulation of proliferation-associated MAPK/ERK1,2 but not that of PCNA. It also promoted PGF and OXT but not P 4 release. Overexpression of STAT1 reversed the effect of ghrelin on STAT1, PCNA, PGF, OXT (from stimulatory to inhibitory), BCL2, P 4 (from inhibitory to stimulatory), prevented ghrelin effect on caspase-3 and BAX, but did not affect ghrelin's effect on MAPK/ERK1,2 expression. These results suggest that ghrelin directly affects porcine ovarian cells function -stimulates proliferation, inhibits apoptosis and affects secretory activity. Furthermore, they demonstrated the involvement of the transcription factor STAT1 in controlling these functions, the promotion of some markers of apoptosis (BAX), inhibition of some markers of proliferation (MAPK/ERK1,2) and stimulation of PGF release. Finally, the obtained data failed to demonstrate that STAT1 is involved in mediating the action of ghrelin on ovarian cell functions.

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Non-genomic effect of steroids on oxytocin-stimulated intracellular mobilization of calcium and on prostaglandin F2α and E2 secretion from bovine endometrial cells

Cited by 35 publications

References 46 publications

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Transcription factor p53 can regulate proliferation, apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH

Involvement of the transcription factor STAT1 in the regulation of porcine ovarian granulosa cell functions treated and not treated with ghrelin

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