Non-invasive assessment of ATP regeneration potential of the preserved donor liver

Changani, K. Kumar; Fuller, Barry; Bryant, David; Bell, Jimmy D.; Ala‐Korpela, Mika; Taylor-Robinson, Simon D; Moore, D.; Davidson, Brian R

doi:10.1016/s0168-8278(97)80050-5

Cited by 41 publications

(37 citation statements)

References 13 publications

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“…In vivo 13 C and 1 H magnetic resonance spectroscopy. Data from 13 C are more difficult to obtain in vivo, mainly because this nucleus has a natural abundance of only 1.1% and the most abundant carbon isotope ( 12 C) is NMR insensitive.…”

Section: Spectral Informationmentioning

confidence: 99%

“…Data from 13 C are more difficult to obtain in vivo, mainly because this nucleus has a natural abundance of only 1.1% and the most abundant carbon isotope ( 12 C) is NMR insensitive. Despite this, signals from glycogen and lipids can be measured using natural abundance 13 C MRS, and quantitative measurements made 3 . However, most studies augment the NMR signal by administered non-radioactive 13 C-labelled tracers, for example 13 C-glucose, to follow metabolic pathways in vivo.…”

Section: Spectral Informationmentioning

confidence: 99%

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Applications of magnetic resonance spectroscopy to chronic liver disease

Taylor-Robinson

2001

Clinical Medicine

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-In vivo magnetic resonance spectroscopy (MRS) provides a non-invasive 'window' on biochemical processes within the body. The technique is currently a research tool, but new developments in whole-body magnetic resonance imaging (MRI) may provide a role for clinical MRS in obtaining functional information at the end of a standard MRI examination. Applications of hepatic and cerebral MRS to chronic liver disease and its associated complications are specifically considered in this article. Changes in phosphorus-31 MRS with the underlying functional severity of the cirrhotic liver are discussed. These reflect increased turnover of cell membranes as the liver attempts to regenerate. Patterns of spectral abnormality in the transplanted liver are described, and also the potential use of the technique to assess the viability of the stored donor liver in the time period between organ harvesting and implantation in the transplant recipient. Metabolite abnormalities in the brain are defined in hepatic encephalopathy and in patients infected with the hepatitis C virus who have minimal hepatic inflammation. Future trends are considered: for example, the use of MRS as a non-invasive tool to assess the effectiveness of liver-directed gene therapy.

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Section: Spectral Informationmentioning

confidence: 99%

Section: Spectral Informationmentioning

confidence: 99%

Applications of magnetic resonance spectroscopy to chronic liver disease

Taylor-Robinson

2001

Clinical Medicine

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“…A typical 31 P MR spectrum, obtained at 1.5 T from a pig liver after 2 hours of cold ischemia followed by a progressive period of oxygenated hypothermic reperfusion (HtR). 73 The NTP resonance is apparent at 5 minutes of HtR; with further HtR, NTP is substantially regenerated. …”

Section: Human and Large Animal Studiesmentioning

confidence: 98%

“…5). 72,73 Livers were reperfused with modified oxygenated UW solution, and timed tissue biopsy samples were taken for in vitro high-resolution MR analysis. Results showed rapid reductions in NTP during cold storage, but upon reperfusion NTP was regenerated to 70% of preharvest values.…”

Section: Human and Large Animal Studiesmentioning

confidence: 99%

Liver transplantation: Current and potential applications of magnetic resonance spectroscopy

et al. 1997

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Magnetic resonance spectroscopy (MRS) allows the noninvasive measurement of whole organ metabolism due to the presence of the MRsensitive nucleus phosphorus 31 in adenosine triphosphate (ATP), its precursors, and breakdown products. In small animal liver transplant studies it has been used to analyze the metabolic effects of cold and warm ischemia, hypothermic reperfusion, and the relative efficacy of different organ preservation solutions. In recent large animal studies MRS has been developed to provide continuous dynamic information on ATP metabolism during graft reperfusion and the bioenergetic consequences of altering preservation solutions. These basic experimental data need to be critically evaluated in human liver transplantation. Encouraging preliminary data on many possible clinical applications have already been obtained, such as the assessment of human donor liver viability and posttransplant graft function. At present, the cost and technically demanding nature of MRS may restrict its application to research units.

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“…In vitro and ex vivo MRS studies of animals have established a model, which follows human org an harvesting and storage protocols [34,35] . Rapid reductions in ATP levels, readily measured by ex vivo 31 P MRS have been seen in a pig model compared to rodent models and levels could be replenished by hypothermic reperfusion [34] .…”

Section: Orthotopic Liver Transplantationmentioning

confidence: 99%

Current and future applications ofin vitromagnetic resonance spectroscopy in hepatobiliary disease

Cox

Sharif²,

Cobbold³

et al. 2006

WJG

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Nuclear magnetic resonance spectroscopy allows the study of cellular biochemistry and metabolism, both in the whole body in vivo and at higher magnetic field strengths in vitro . Since the technique is non-invasive and non-selective, magnetic resonance spectroscopy methodologies have been widely applied in biochemistry and medicine. In vitro magnetic resonance spectroscopy studies of cells, body fluids and tissues have been used in medical biochemistry to investigate pathophysiological processes and more recently, the technique has been used by physicians to determine disease abnormalities in vivo . This highlighted topic illustrates the potential of in vitro magnetic resonance spectroscopy in studying the hepatobiliary system. The role of in vitro proton and phosphorus magnetic resonance spectroscopy in the study of malignant and non-malignant liver disease and bile composition studies are discussed, particularly with reference to correlative in vivo whole-body magnetic resonance spectroscopy applications. In summary, magnetic resonance spectroscopy techniques can provide non-invasive biochemical information on disease severity and pointers to underlying pathophysiological processes. Magnetic resonance spectroscopy holds potential promise as a screening tool for disease biomarkers, as well as assessing therapeutic response.

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Non-invasive assessment of ATP regeneration potential of the preserved donor liver

Cited by 41 publications

References 13 publications

Applications of magnetic resonance spectroscopy to chronic liver disease

Applications of magnetic resonance spectroscopy to chronic liver disease

Liver transplantation: Current and potential applications of magnetic resonance spectroscopy

Current and future applications ofin vitromagnetic resonance spectroscopy in hepatobiliary disease

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