Non-invasive bat species identification from mixed-species samples using a microarray

Patrick, Lorelei E.; Just, Jan; Vonhof, Maarten J.

doi:10.1007/s12686-016-0613-0

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…The two markers identified by Zinck et al (2004) were recently applied to DNA from mixed guano samples collected in Canada and the northern United States using microarray technology, allowing for the genetic identification of 34 bat species (Patrick et al 2016). The sequences we present, along with those determined by Patrick et al (2016) and Zinck et al (2004), create a large 16S mtDNA sequence database that has broad application for bat-related research. This database and the genetic analysis techniques we employed will be useful to identify bat species in areas where, as a result of WNSrelated declines or the cryptic nature of many bat species, roost surveys, mist netting, or acoustic monitoring are ineffective.…”

Section: Discussionmentioning

confidence: 99%

“…When bat species are rare, elusive, or share similar morphological or call characteristics, species identification using genetic markers from fecal material (i.e., guano) found in or around roost locations (i.e., caves, trees, and buildings) may provide an alternative indicator of occurrence (Patrick et al 2016;Walker et al 2016;Zinck et al 2004) identified two genetic markers from 16S ribosomal subunit, mitochondrial deoxyribonucleic acid (mtDNA), for bat species identification using fecal DNA and applied this marker to DNA extracted from guano of bats found in the northwestern United States. Only 5 of the 14 species sequenced by Zinck et al (2004) are found in the southeastern United States: Rafinesque's big-eared bat Corynorhinus townsendii, big brown bat Eptesicus fuscus, silver-haired bat Lasionycteris noctivagans, hoary bat Lasiurus cinereus, and little brown myotis.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Southeastern Bat Species Using Noninvasive Genetic Sampling of Individual Guano Pellets

Brown

Willcox

Fagan

et al. 2017

Journal of Fish and Wildlife Management

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The impact of white-nose syndrome on North American bat populations may limit the effectiveness of traditional monitoring methods, including roost surveys, mist netting, and acoustic monitoring, and, in turn, determination of bat species occurrence. Genetic markers from deoxyribonucleic acid (DNA) extracted from feces (i.e., guano pellets) may provide an effective alternative method for assessing occurrence. We used an existing genetic marker from the 16S ribosomal subunit, mitochondrial DNA, to create a DNA sequence database for the 16 species of bats known to occur in Tennessee. We used our database to identify bat species from DNA extracted from 68 guano pellets collected from accumulations found in buildings of Great Smoky Mountains National Park from May to August 2015. No bats were directly observed at 19 roost buildings (55.9% of all identified roost buildings), where genetic analysis of guano was the only method available to determine species occurrence. Two of the species we detected roosting in buildings using DNA from guano, the little brown myotis Myotis lucifugus and northern long-eared myotis M. septentrionalis, are of special concern as a result of declines from white-nose syndrome. There are no records of the northern long-eared myotis roosting in Great Smoky Mountains National Park buildings, and no records of the little brown myotis roosting in buildings since white-nose syndrome became established in Great Smoky Mountains National Park. Our findings emphasize the utility of these genetic techniques for detecting bat species when visual or acoustic methods may be compromised by species rarity, elusive behavior, or similarities in species morphology and call characteristics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Southeastern Bat Species Using Noninvasive Genetic Sampling of Individual Guano Pellets

Brown

Willcox

Fagan

et al. 2017

Journal of Fish and Wildlife Management

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Developmental validation of SpeID: A pyrosequencing-based assay for species identification

Ghemrawi

Fischinger²,

Duncan

et al. 2021

Forensic Science International: Genetics

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Genetic assays for guano-based identification of species and sex in bats of the United States and Canada

Guan

Britzke

Piaggio

et al. 2020

Journal of Mammalogy

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Bat guano is a noninvasive, data-rich genetic resource. However, the constituent bat DNA is relatively scant, degraded, and complexed with polymerase chain reaction inhibitors. It also is comingled with a rich pool of nontarget DNA from microbes, parasites, and dietary items. We designed and tested new DNA assays for bat species identification (COX1-Bat) and sex identification (XGXYC) for use with guano and other challenging samples. We reviewed previously published assays that can be used with guano samples to obtain the same species and sex data, and attempted to validate these assays for species in which they had not previously been tested. Our results demonstrate that guano-derived DNA can be used successfully to 1) identify nearly all US and Canadian bats at the species level, or to one of three Myotis species clusters, and 2) identify the sex of at least 23 US and Canadian bat species. Our newly developed assays, and validation of previously published assays, for guano-based identification of species and sex in bats, significantly enhance the power of noninvasive sampling and genetic analysis for bat studies, management, and conservation.

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Non-invasive bat species identification from mixed-species samples using a microarray

Cited by 3 publications

References 42 publications

Identification of Southeastern Bat Species Using Noninvasive Genetic Sampling of Individual Guano Pellets

Identification of Southeastern Bat Species Using Noninvasive Genetic Sampling of Individual Guano Pellets

Developmental validation of SpeID: A pyrosequencing-based assay for species identification

Genetic assays for guano-based identification of species and sex in bats of the United States and Canada

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