Non-Invasive Delivery of dsGST Is Lethal to the Sweet Potato Whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae)

Asokan, R.; Rebijith, K. B.; Roopa, H. K.; Kumar, N. K. Krishna

doi:10.1007/s12010-014-1437-6

Cited by 22 publications

(9 citation statements)

References 71 publications

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“…56 The sucrose diet containing 200 ng/ml dsGFP was used as a negative control. The dsRNA was synthesized using AmpliScribeTM T7-Flash Transcription kit (Epicentre, ASF3507).…”

Section: Rna Interferencementioning

confidence: 99%

The autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies

et al. 2016

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Macroautophagy/autophagy plays an important role against pathogen infection in mammals and plants. However, little has been known about the role of autophagy in the interactions of insect vectors with the plant viruses, which they transmit. Begomoviruses are a group of single-stranded DNA viruses and are exclusively transmitted by the whitefly Bemisia tabaci in a circulative manner. In this study, we found that the infection of a begomovirus, tomato yellow leaf curl virus (TYLCV) could activate the autophagy pathway in the Middle East Asia Minor 1 (MEAM1) species of the B. tabaci complex as evidenced by the formation of autophagosomes and ATG8-II. Interestingly, the activation of autophagy led to the subsequent degradation of TYLCV coat protein (CP) and genomic DNA. While feeding the whitefly with 2 autophagy inhibitors (3-methyladenine and bafilomycin A 1 ) and silencing the expression of Atg3 and Atg9 increased the viral load; autophagy activation via feeding of rapamycin notably decreased the amount of viral CP and DNA in the whitefly. Furthermore, we found that activation of whitefly autophagy could inhibit the efficiency of virus transmission; whereas inhibiting autophagy facilitated virus transmission. Taken together, these results indicate that TYLCV infection can activate the whitefly autophagy pathway, which leads to the subsequent degradation of virus. Furthermore, our report proves that an insect vector uses autophagy as an intrinsic antiviral program to repress the infection of a circulative-transmitted plant virus. Our data also demonstrate that TYLCV may replicate and trigger complex interactions with the insect vector.

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“…56 The sucrose diet containing 200 ng/ml dsGFP was used as a negative control. The dsRNA was synthesized using AmpliScribeTM T7-Flash Transcription kit (Epicentre, ASF3507).…”

Section: Rna Interferencementioning

confidence: 99%

The autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies

et al. 2016

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“…As, for now, we are unable to generate Dys mutants in whiteflies, we used a dsRNA‐based RNAi gene silencing approach to study the role of Dys during B. tabaci development. Several dsRNA delivery systems have been used to silence insect genes: by microinjection (Ghanim et al , ; Ren et al , ), through the diet (Asokan et al , ; Jia et al , ), by topical application (Killiny et al ., ), by leaflet absorption (Luan et al ., ; Camargo et al , ) and by expressing dsRNA in transgenic plants (Thakur et al ., ; Reddy and Rajam, ; Knorr et al , ). Here, to deliver the silencing Dys dsRNA to whiteflies, we took advantage of the fact that in order to feed, the developing insects (from crawlers to adults) insert their stylets into the leaf vascular system.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of dystrophin expression in pupae of the whitefly Bemisia tabaci inhibits the emergence of adults

Bar

Czosnek

Sobol

et al. 2019

Insect Molecular Biology

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The whitefly Bemisia tabaci is a major pest to agriculture. Adults are able to fly for long distances and to colonize staple crops, herbs and ornamentals, and to vector viruses belonging to several important taxonomic groups. During their early development, whiteflies mature from eggs through several nymphal stages (instars I to IV) until adults emerge from pupae. We aim at reducing whitefly populations by inhibiting the emergence of adults from nymphs. Here we targeted dystrophin, a conserved protein essential for the development of the muscle system in humans, other animals and insects. We have exploited the fact that whitefly nymphs developing on tomato leaves feed from the plant phloem via their stylets. Thus, we delivered dystrophin‐silencing double‐stranded RNA to nymphs developing on leaves of tomato plantlets with their roots bathing in the silencing solution. Downregulation of dystrophin expression occurred mainly in pupae. Dystrophin silencing induced also the downregulation of the dystrophin‐associated protein genes actin and tropomyosin, and disrupted F‐actin. Most significantly, the treatment inhibited the emergence of adults from pupae, suggesting that targeting dystrophin may help to restrain whitefly populations. This study demonstrates for the first time the important role of dystrophin in the development of a major insect pest to agriculture.

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“…Glutathione S ‐transferases ( GST ) are known to play a pivotal role in the detoxification of secondary allelochemical and in insecticide resistance. Asokan et al () explored the potential of RNAi in B. tabaci by targeting the gst gene. After sequencing the gst gene from B. tabaci , ~500 basepairs‐long dsRNA targeting this gene was synthesized and delivered to B. tabaci via the oral route.…”

Section: Rnai As a Tool To Research Or Interfere With Insecticide Resmentioning

confidence: 99%

Potential of RNA interference in the study and management of the whitefly, Bemisia tabaci

Grover

Jindal

Banta

et al. 2018

Arch Insect Biochem Physiol

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Whiteflies cause considerable losses to crops, directly by feeding, and indirectly by transmission of viruses. The current control methods consist of a combination of different control tactics, mainly still relying on unsafe and non-ecofriendly chemical control. RNA interference (RNAi) is a post-transcriptional gene-silencing strategy in which double-stranded RNA (dsRNA), corresponding specifically to a target gene, is introduced in a target organism. Research on RNAi in the previous decade has shown its success as a potential insect control strategy, which can be highly species-specific and environment friendly. In whiteflies, the success of dsRNA delivery through the oral route opened possibilities for its management through plant-mediated RNAi. To date, several genes have been targeted in whiteflies through RNAi and these assays demonstrated its potential to manage whiteflies at lab level. However, further research and investments are needed to move toward an application at field level. In this review, for the first time, we collected the literature on genes targeted for silencing via RNAi in whiteflies and discuss the potential of RNAi in whitefly pest control. We also discuss likely delivery methods, including transgenic in planta delivery and symbiont-mediated delivery, and its potential for studying and interfering with insecticide resistance mechanisms and virus transmission by whiteflies. K E Y W O R D S endosymbionts, functional genomics, resistance mechanism, RNA interference, whitefly Arch. Insect Biochem. Physiol. 2019;100:e21522.wileyonlinelibrary.com/journal/arch

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Non-Invasive Delivery of dsGST Is Lethal to the Sweet Potato Whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae)

Cited by 22 publications

References 71 publications

The autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies

The autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies

Downregulation of dystrophin expression in pupae of the whitefly Bemisia tabaci inhibits the emergence of adults

Potential of RNA interference in the study and management of the whitefly, Bemisia tabaci

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