Non‐invasive longitudinal monitoring of angiogenesis in a murine full‐thickness cutaneous wound healing model using high‐resolution three‐dimensional ultrasound imaging

Mukai, Kanae; Zhu, Weipei; Nakajima, Yukari; Kobayashi, Masayoshi; Nakatani, Toshio

doi:10.1111/srt.12374

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…This finding proves that diabetic wounds tended to form scars, and DP treatment inhibited the formation of wound scars. The microvessel density results also confirmed that DP accelerated the regeneration of new blood vessels, delivered nutrients to the wound, and accelerated wound healing [ 43 ]. DP can promote angiogenesis by increasing the expression of N, which can stimulate endothelial cells and macrophages to secrete vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) [ 44 ].…”

Section: Discussionmentioning

confidence: 72%

Dracorhodin Perchlorate Regulates the Expression of Inflammatory Cytokines through the TLR4 Pathway and Improves Skin Wound Healing in Diabetic Rats

Xiong

Huo

Jia

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Dragon’s blood is a natural medicine with hemostatic and blood-activating effects and is used to promote wound healing. Dracorhodin perchlorate (DP) is a stable form of dracarhod and is used as a substitute for cochinchinenin. DP promotes the proliferation of rat fibroblasts and promotes wound healing in rats. Methods. DP ointment (0.2 mg/mL) was applied to the skin wounds of nondiabetic and diabetic rats, and the skin of the wound was collected. Wound healing rate, H&E staining, Masson staining, TLR4 pathway, related inflammatory factors, nitric oxide synthase, and so forth were detected. Results. DP treatment alleviated the prolonged inflammatory cell infiltration time and the increase in the TLR4 pathway and inflammatory factors caused by diabetes. DP also promoted wound healing by increasing eNOS protein expression and NO content in the later stage of wound healing. Conclusion. DP promotes wound healing in diabetic rats by regulating the TLR4 pathway and related inflammatory factors. Therefore, adjuvant treatment of DP can be developed for diabetic wound healing.

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Section: Discussionmentioning

confidence: 72%

Dracorhodin Perchlorate Regulates the Expression of Inflammatory Cytokines through the TLR4 Pathway and Improves Skin Wound Healing in Diabetic Rats

Xiong

Huo

Jia

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Microvessel formation plays an important role in the proliferation of epithelial cells during wound healing. Vascular regeneration is also an important indicator of wound healing [37][38][39][40]. Among various substances related to the promotion of angiogenesis, VEGF plays an extremely important role.…”

Section: Discussionmentioning

confidence: 99%

Patching Retinal Breaks with Chitosan for Retinal Detachment in Rabbits

Wei¹,

Jiang²

2023

Discovery Medicine

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Background: Rhegmatogenous retinal detachment (RRD) is caused by one or more full-thickness retinal breaks. The current RRD treatments have several drawbacks. Chitosan is one of the most commonly used natural polymers for wound healing and has been demonstrated to be biodegradable, biocompatible, non-toxic, bioadhesive, and bioactive. This study aimed to determine the reliability and effectiveness of chitosan for sealing retinal breaks in rabbits. Methods: Eighteen blue purple rabbits were randomly divided into three groups: chitosan (n = 6), RRD (n = 6), and control (n = 6). The RRD model was established using vitrectomy, making retinal holes, and subretinal fluid injection in the RRD and chitosan groups. One week after the establishment of the model, chitosan was applied within the range of the holes in the chitosan group, and the vitreous body was filled with perfusion fluid. Except the chitosan treatment, the RRD group underwent the same procedure. Intraocular pressure (IOP) measurement, fundus photography, B-mode ultrasound, optical coherence tomography (OCT), histology, and enzyme linked immunosorbent assay (ELISA) were performed. Results: Retinas of all eyes in the RRD group were detached, whereas those of all eyes in the chitosan group remained attached. The concentrations of epidermal growth factor (EGF), fibroblast growth factor (FGF)-2, transforming growth factor β (TGF-β), vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), and IL-8 in the vitreous fluid of the RRD group were significantly higher than those of the control group (p < 0.05). Furthermore, the concentrations of EGF, FGF-2, TGF-β, and VEGF in the vitreous fluid of the chitosan group were higher compared to those of the RRD group (p < 0.05), whereas the concentrations of IL-6 and IL-8 were lower (p < 0.05). Conclusions: Chitosan may be a reliable method for sealing retinal breaks. Moreover, chitosan can maintain high levels of growth factors and reduce inflammatory factors in the vitreous, which may reduce and delay the death of retinal cells and help restore visual function after retinal repositioning.

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Advanced Wound Diagnostics: Toward Transforming Wound Care into Precision Medicine

Weigelt

Lev‐Tov

Tomic‐Canic

et al. 2022

Advances in Wound Care

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Non‐invasive longitudinal monitoring of angiogenesis in a murine full‐thickness cutaneous wound healing model using high‐resolution three‐dimensional ultrasound imaging

Abstract: These results indicate that high-resolution 3D ultrasound imaging is useful for longitudinally evaluating the distribution of microvascular growth over the course of healing.

Cited by 3 publications

References 27 publications

Dracorhodin Perchlorate Regulates the Expression of Inflammatory Cytokines through the TLR4 Pathway and Improves Skin Wound Healing in Diabetic Rats

Dracorhodin Perchlorate Regulates the Expression of Inflammatory Cytokines through the TLR4 Pathway and Improves Skin Wound Healing in Diabetic Rats

Patching Retinal Breaks with Chitosan for Retinal Detachment in Rabbits

Advanced Wound Diagnostics: Toward Transforming Wound Care into Precision Medicine

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