Non-Invasive Measurement of Cerebral Oxygen Metabolism in the Mouse Brain by Ultra-High Field <sup>17</sup>O MR Spectroscopy

Cui, Weina; Zhu, Xiao Hong; Vollmers, Manda L.; Colonna, Emily T.; Adriany, Gregor; Tramm, Brandon; Dubinsky, Janet M.; Öz, Gülin

doi:10.1038/jcbfm.2013.172

Cited by 31 publications

(46 citation statements)

References 14 publications

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“…It has been impractical to perform 31 P-MRSI at low-field clinical scanners with adequate resolution and scan time. Preclinical 31 P and other hetero-nuclei MRSI studies are commonly performed at high fields for SNR gain (53)(54)(55). However, the requirement of high spatial resolution for small animal imaging still renders 31 P-MRSI challenging.…”

Section: Historical Perspectivementioning

confidence: 99%

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Liu

2017

Quant. Imaging Med. Surg.

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Many human diseases are caused by an imbalance between energy production and demand.Magnetic resonance spectroscopy (MRS) and magnetic resonance imaging (MRI) provide the unique opportunity for in vivo assessment of several fundamental events in tissue metabolism without the use of ionizing radiation. Of particular interest, phosphate metabolites that are involved in ATP generation and utilization can be quantified noninvasively by phosphorous-31 ( 31 P) MRS/MRI. Furthermore, 31 P magnetization transfer (MT) techniques allow in vivo measurement of metabolic fluxes via creatine kinase (CK) and ATP synthase. However, a major impediment for the clinical applications of 31 P-MRS/MRI is the prohibitively long acquisition time and/or the low spatial resolution that are necessary to achieve adequate signal-to-noise ratio. P-MRS/MRI techniques. Applications of these techniques to the investigation of a specific physiology/pathology will be discussed without detailed description. Interested readers are referred to recent review articles on the applications of 31 P-MRS/MRI in metabolic characterization of skeletal muscle (10-12), heart (13), brain (14), and liver (11), as well as in diseases such as cancer (15), heart failure (16), and obesity and diabetes (17,18). Historical perspectiveThe use of 31 P-MRS for metabolic investigations dates back to 1960. Cohn and Hughes were the first to obtain a high-resolution 31 P spectrum of a solution of ATP (19). In addition, they also observed a dependence of the chemical shifts of the phosphorus nuclei of ATP on the pH of the solution. In parallel to the early development of MRI methods by Lauterbur (20), the entire 1970s also witnessed the rapid advance of 31 P-MRS in metabolic investigation of a broad range of biological systems. In 1973, Moon and Richards performed the first 31 P-MRS study that measured intracellular pH in human red blood cells (21). In the following year, Henderson and colleagues obtained the first 31 P spectrum of ATP from human red blood cells (22). At the same time, the Oxford team led by Radda performed the first experiment to acquire 31 P spectra from an intact organ, i.e., the excised and superfused muscle of rat hindlimb (23). The first in vivo 31 P-MRS study was performed on mouse brain by Chance et al. (24). Within the short span of one decade, organelles including mitochondria (25,26) and chromaffin granules (27), cells including Escherichia coli (28), yeast (29), and hepatocytes (30), and organs including skeletal muscle (31,32), heart (33-35), brain (36), kidney (37), and liver (38,39) have all been studied using 31 P-MRS.It is worth noting that most of these organ studies were performed on excised organs to avoid the need for spatial localization. Although Lauterbur has demonstrated the feasibility of imaging water protons (20), it was considered impractical for 31 P imaging of living systems because of the low sensitivity and difficulties with spectral resolution.The 1980s began with the publication of two important studies that aimed a...

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Section: Historical Perspectivementioning

confidence: 99%

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Liu

2017

Quant. Imaging Med. Surg.

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“…Therefore, the decreased SNR can be compensated with increased signal averages, as the kinetics of 17 O signal in an inhalation study can be fully captured with a lower temporal resolution. A previous study on mice suggests that a 15-s temporal resolution is adequate for an inhalation study (9). Achieving this temporal resolution would allow the current study to collect twice as many signal averages to compensate for the reduced SNR due to using inhaled 17 O 2 .…”

Section: Discussionmentioning

confidence: 99%

“…In the current study, we were able to achieve an effective temporal resolution of 7.56 s with a voxel size of 11.25 mL (5.625 mL nominal) at 9.4 T. The flexibility of choosing a k-space filter to achieve adequate temporal resolution allows application of this method to quantify CMRO 2 in 17 O 2 inhalation studies. For a typical 17 O 2 inhalation study, only a small amount of gas is delivered in $2 min to reduce the cost for 17 O 2 (8,9). Having a reduced amount of 17 O 2 available in vivo causes reduced signal increases, leading to lower SNR at peak and steady-state.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, using a short echo time to minimize signal loss in 17 O-MRI is critical due to the short transverse relaxation time of 17 O (5). 17 O-MRI has been used extensively to evaluate cerebral metabolic activity in rats (6), healthy cats (7), and mice (8,9). Quantification of the cerebral metabolic rate of oxygen consumption (CMRO 2 (8,10,11).…”

Section: Introductionmentioning

confidence: 99%

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High‐resolution dynamic oxygen‐17 MR imaging of mouse brain with golden‐ratio–based radial sampling and k‐space–weighted image reconstruction

Liu

Zhang

et al. 2017

Magnetic Resonance in Med

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Purpose: The current study aimed to develop a three-dimensional (3D) dynamic oxygen-17 ( 17 O) MR imaging method with high temporal and spatial resolution to delineate the kinetics of 17 O water uptake and washout in the brains of mice with glioblastoma (GBM). Methods: A 3D imaging method with a stack-of-stars goldenratio-based radial sampling scheme was employed to acquire 17 O signal in vivo. A k-space-weighted image reconstruction method was used to improve the temporal resolution while preserving spatial resolution. Simulation studies were performed to validate the method. Using this method, the kinetics of 17 O water uptake and washout in the brains of mice with GBM were delineated after an intravenous bolus injection of 17 O water kinetics in vivo with high temporal and spatial resolution. It can also be used to image cerebral oxygen consumption rate in oxygen-17 inhalation studies.

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“…Using a quasisteady state approximation, the difference in T x obtained from above equation at baseline and during epilepsy gave an estimate of the initial dip versus distance from the vessel. For the mouse brain, the CMRO 2 was assumed to be 2.6 μmol∕g∕ min at baseline 18 and to increase ∼12% during epileptic seizures. 10 Measured changes in T 0 during seizures were used in the model.…”

Section: Simulation Of Oxygen Diffusion In Tissuementioning

confidence: 99%

Spatial landscape of oxygen in and around microvasculature during epileptic events

2017

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Abstract. Measuring changes in cerebral oxygen in tissue microdomains during epilepsy is important to identify hypoxic potential. Here, using a custom-built two-photon microscopy system, we present microscopic measurements of oxygen partial pressure (PO 2 ) in cortical microvessels and tissue of anesthetized mice during 4-aminopyridine (4-AP)-induced epileptic seizures. Investigating epileptic events, we characterized the distribution of the "initial dip" in PO 2 in arterioles, venules, and tissue near the 4-AP injection site. Our results reveal a correlation between the percent change in PO 2 during the "initial dip" and the diameter of nearest arterioles and venules.

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Non-Invasive Measurement of Cerebral Oxygen Metabolism in the Mouse Brain by Ultra-High Field ¹⁷O MR Spectroscopy

Cited by 31 publications

References 14 publications

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

High‐resolution dynamic oxygen‐17 MR imaging of mouse brain with golden‐ratio–based radial sampling and k‐space–weighted image reconstruction

Spatial landscape of oxygen in and around microvasculature during epileptic events

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Non-Invasive Measurement of Cerebral Oxygen Metabolism in the Mouse Brain by Ultra-High Field 17O MR Spectroscopy

Cited by 31 publications

References 14 publications

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

High‐resolution dynamic oxygen‐17 MR imaging of mouse brain with golden‐ratio–based radial sampling and k‐space–weighted image reconstruction

Spatial landscape of oxygen in and around microvasculature during epileptic events

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Non-Invasive Measurement of Cerebral Oxygen Metabolism in the Mouse Brain by Ultra-High Field ¹⁷O MR Spectroscopy