Non-Invasive Measurement of Cortisol Metabolite in Feces of Toraya Buffalo by Using Enzyme Immunoassay Technique

Abstract

Non-invasive measurement hormones in feces using enzyme immunoassay (EIA) technique needs to be validated. This study was conducted to develop and validate an enzyme immunoassay kit for measuring the concentration of cortisol metabolites in feces of Toraya buffalo. An EIA kit of 11β-hydroxyetiocholanolone (11β-hydroxy-CM assay) was developed and validated analytically and biologically using feces of Toraya Buffalo for cortisol metabolite measurements. Analytical validation comprises the parallelism test, accuracy, precision, and assay sensitivity. Biological validation performed by comparing concentration cortisol metabolites in feces of Toraya buffalo before and after pa’silaga tedong, a fighting contest of buffalo during the death ceremony of rambu solo at Tana Toraja, South Sulawesi Province. Results showed that the curve of serial dilution of fecal samples was parallel with the 11β-hydroxy-CM standard curves. The accuracy and sensitivity of the 11β-hydroxy-CM assay were 96.21%±7.59 and 0.78pg/well, respectively. The precision based on coefficient variation (CV) of intraand inter-assay was < 10% and < 15%, respectively. Biological validation results showed that cortisol metabolites concentrations after pa’silaga tedong were increased 3-10 fold than before pa’silaga tedong. In conclusion, the 11β-hydroxy-CM assay is a reliable assay for measuring cortisol metabolites in feces of Toraya buffalo.

Effect of Visitor Number on the Behavior and Cortisol Metabolites Concentrations of Sambar Deer (Cervus unicolor) in Captivity

“…This is because the 2 nd to 5 th week of observation is the school holidays. This result in line with a study that reported one of the factors of visitor attendance is a school holiday [15]. The sex of visitors is 51% male and 49% female which they visited the Sambar deer with a family group.…”

Transplantation of Aceh cattle ovary into the uterus of pseudopregnant local rabbits: Effect of post-transplant stress on uterine histopathology and ovarian follicle dynamics

“…The mean cortisol levels (ng/g) recorded before versus after transplantation on days 3, 5, and 7 were 146. 23 In this study, the transplant procedure was considered to be successful because some follicles remained in the various stages of development. This is in accordance with the report of Sumarmin et al [38], who stated that the success of the transplantation can be proved by the discovery of follicles; in fact, the presence of follicles proves that the epithelial structure that protects the ovarian cortex is intact, thus ensuring that metabolism can occur normally.…”

“…Fecal samples were extracted using a method described by Gholib et al . [ 23 ]. Before the extraction process, all frozen fecal samples were thawed at 50°C for 1–2 h. Then, the samples were homogenized and ~0.55 g of the fresh fecal samples was placed in tubes containing 4.5 mL of 80% methanol.…”

“…A fecal sample was collected immediately after defecation, and 5-10 g was placed in a sample tube and then stored in a freezer at -20°C before extraction. Fecal samples were extracted using a method described by Gholib et al [23]. Before the extraction process, all frozen fecal samples were thawed at 50°C for 1-2 h. Then, the samples were homogenized and ~0.55 g of the fresh fecal samples was placed in tubes containing 4.5 mL of 80% methanol.…”

Pengaruh Transplantasi Ovarium pada Kelinci Lokal Bunting Semu Terhadap Peningkatan Hormon Kortisol

“…Sampel feses diekstraksi menggunakan metode seperti yang dilaporkan Gholib et al (2020). Tahap pertama sampel di-thawing pada suhu 50°C selama 1-2 jam.…”