Non-invasive Point-of-Care Device To Diagnose Acute Mesenteric Ischemia

Abstract

Inadequate blood supply to the intestine can lead to acute mesenteric ischemia (AMI), with a mortality rate ranging from 60% to 90%. This high mortality rate is partially due to late detection and the lack of efficient early diagnostic tests. There is an urgent need for a point-of-care tool for immediate bedside diagnosis. Here we present for the first time a rapid and non-invasive electrochemical biosensor device based on nonfaradic impedance spectroscopy to detect intestinal fatty-acid binding protein (I-FABP) as an indication of AMI. The electrochemical biosensors consist of gold interdigitated electrodes that were fabricated using photolithographic techniques on top of silicon dioxide substrates. The electrode surfaces were functionalized with an I-FABP capture antibody (CAnB) to entice the target protein, while gold nanoparticles (GNPs) functionalized with detection antibodies (DAnB-GNPs) were utilized as a novel mechanism to enhance the detection signal. Quantification of the I-FABP concentration in the medium depended on its attachment to CAnB and DAnB-GNPs in a sandwich manner, where the latter boosts the impedance signal through its binding to the I-FABP. This non-invasive non-faradic electric biosensor device demonstrates the potential for bench-to-bedside translation with the goal of decreasing morbidity and mortality from AMI.

Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

“…Once the spores are captured, non-faradaic electrochemical impedance spectroscopy (nF-EIS) measurements can be performed to quantify the spores column by column. nF-EIS is a label-free detection technique that measures the electrical current of an electrode–electrolyte system in response to an applied AC potential with no redox species in solution. − During nF-EIS measurements (Figure f), nanoelectrodes can be operated individually, allowing the measurement of each column in the microwell array and providing the ability to determine spore occupancy per column.…”

Improving immunoassay detection accuracy of anti-SARS-CoV-2 antibodies through dual modality validation

“…Borofloat glass wafers were purchased from Swift Glass Co., Inc. Elmira Heights NY, and sliced as substrates for the deposition of IDEs and surface modification. IDEs were deposited through a standard photolithographic method as reported before ( Abdelrasoul et al, 2018 ). SARS-CoV-2 spike protein was provided by , with the method of immobilization briefly described in the supporting information.…”

Electrochemical (Bio)Sensing Devices for Human Microbiome Related Biomarkers

“…Determining the levels of this protein in serum or plasma provides information about intestinal barrier dysfunction. In addition, its detection in urine or blood has been reported as a promising non-invasive method for identifying patients with acute mesenteric ischemia (AMI), provoked by an inadequate blood supply to the intestine [70]. An electrochemical biosensor for iFABP was fabricated using gold interdigitated electrodes functionalized with the specific capture antibody and sandwiching the protein with a gold nanoparticles-modified detection antibody.…”