Non-mendelian inheritance of mitochondrial DNA and ribosomal DNA in the myxomycete, Didymium iridis

Silliker, Margaret E.; Collins, O’Neil Ray

doi:10.1007/bf00339605

Cited by 24 publications

(12 citation statements)

References 35 publications

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“…In D. iridis, the inheritance mode of mitochondria has been analyzed. The results showed that the inheritance mode of mitochondria is predominantly uniparental (Silliker and Collins 1988;Silliker et al 2002, Sheer andSilliker 2006). The uniparental inheritance of organelle is a widely conserved phenomenon in many organisms, and as the 2 Myxomycetes P. polycephalum and D. iridis are relatively closely related species, it could be expected that they possess the same mechanism for the uniparental inheritance of mitochondria.…”

Section: Discussionmentioning

confidence: 93%

“…In this study, we observed the behavior of mtDNA during the mating of Myxomycete D. iridis, to investigate that selective disappearance of mtDNA is a conserved mechanism for the uniparental inheritance of mitochondria, at least in Myxomycetes. The inheritance mode of mtDNA has already been reported in D. iridis, and it is strictly uniparental (Silliker and Collins 1988, Silliker et al 2002, Sheer and Silliker 2006. The life cycle of D. iridis includes 2 distinct vegetative forms: the haploid myxamoeba and the diploid plasmodium.…”

mentioning

confidence: 86%

“…Further, the multiple allelic mating systems common in heterothallic Myxomycetes (Betterley and Collins 1983, Kawano et al 1987, Kawano and Kuroiwa 1989 mean that mitochondrial donor and recipient strains cannot be simply designated by mating type as would be possible in a binary mating system (Hurst 1992, Hurst andHamilton 1992). Despite these features, uniparental inheritance occurs in D. iridis (Silliker and Collins 1988, Silliker et al 2002, Sheer and Silliker 2006. Therefore, an active mechanism that eliminates mitochondria or mtDNA from 1 parent must be involved.…”

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confidence: 99%

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Disappearance of mtDNA During Mating of the True Slime Mold Didymium iridis

et al. 2009

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SummaryThe active, selective digestion of mitochondrial DNA (mtDNA) from 1 parent in the zygote is a possible molecular mechanism for the uniparental inheritance of mitochondria, but direct evidence has been observed in few species. In this study, we observed the behavior of mitochondria and mtDNA during mating of the myxamoeba of the true slime mold Didymium iridis. To show the selective digestion of mtDNA in the zygote, 2 myxamoebal strains of D. iridis were crossed, and the changes of mitochondria and mtDNA were observed over time by phase-contrast observation using alkaline fixation method and DAPI staining. Each myxamoeba of D. iridis contained about 30 mitochondria, and the zygote had about 60. Each mitochondrion contains rod-shaped mtDNA. About 4.5 h after mating, the fluorescence of mtDNA in about 30 mitochondria decreased simultaneously to give small spots, and then disappeared completely by 5 h after mating. In contrast, the mtDNA in the other 30 mitochondria and all of the mitochondrial sheaths remained unchanged. This is the fourth microscopic report that shows selective mtDNA disappearance. The rapid, selective disappearance of mtDNA observed in D. iridis is likely the result of selective digestion of mtDNA from 1 parent, as in other known cases of mtDNA disappearance.

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Section: Discussionmentioning

confidence: 93%

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confidence: 86%

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confidence: 99%

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Disappearance of mtDNA During Mating of the True Slime Mold Didymium iridis

et al. 2009

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“…Because no cell division takes place, mitochondrial segregation does not occur. However, uniparental inheritance of mitochondria has also been reported in the isogamous protists P. polycephalum (Kawano et al 1987a, b;Kawano and Kuroiwa 1989;Meland et al 1991) and D. iridis (Silliker and Collins 1988;Silliker et al 2002). Loss of paternal mitochondria is presumed to happen at an early stage of zygote differentiation despite gametes of equal size being involved, and the zygote develops without Fig.…”

Section: Maternal Inheritance and The Selective Digestion Hypothesis:mentioning

confidence: 89%

Maternal inheritance of mitochondria: multipolarity, multiallelism and hierarchical transmission of mitochondrial DNA in the true slime mold Physarum polycephalum

Moriyama

Kawano

2010

J Plant Res

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Direct evidence of digestion of paternal mitochondrial DNA (mtDNA) has been found in the true slime mold Physarum polycephalum. This is the first report on the selective digestion of mtDNA inside the zygote, and is striking evidence for the mechanism of maternal inheritance of mitochondria. Moreover, two mitochondrial nuclease activities were detected in this organism as-candidates for the nucleases responsible for selective digestion of mtDNA. In the true slime mold, there is an additional-feature of the uniparental inheritance of mitochondria.Although mitochondria are believed to be inherited from the maternal lineage in nearly all eukaryotes, the mating types of the true slime mold P. polycephalum is not restricted to two: there are three mating loci--matA, matB,and matC--and these loci have 16, 15, and 3 alleles,-respectively. Interestingly, the transmission patterns of mtDNA are determined by the matA locus, in a hierarchical-fashion (matA hierarchy) as follows: matA7[matA2[matA11[matA12[matA15/matA16[matA1[matA6.The strain possessing the higher status of matA would be the mtDNA donor in crosses. Furthermore, we have found that some crosses showed biparental inheritance of mitochondria.This review describes the phenomenon of hierarchical transmission of mtDNA in true slime molds, and discusses the presumed molecular mechanism of maternal and biparental inheritance.

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“…Furthermore, the fact that both parental rDNA types are present at the same time, as shown by the polymorphism of the intronindependent marker, strongly favours intron mobility. In a previous study, Silliker and Collins (1988) analysed the inheritance of extrachromosomal rDNAs in D. iridis. They used two parental strains (Pan2-16 and Hon1-2) similar to those presented in this work, and found that the Pan2-16 parental rDNA type was preferentially inherited in all crosses.…”

Section: Discussionmentioning

confidence: 99%

In vivo mobility of a group I twintron in nuclear ribosomal DNA of the myxomycete Didymium iridis

Johansen

Elde

Vader

et al. 1997

Molecular Microbiology

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SummaryDiSSU1 is an optional group I twintron present in the nuclear extrachromosomal ribosomal DNA of the myxomycete Didymium iridis. DiSSU1 appears to be complex both in structure and function. At the RNA level it has a twin-ribozyme organization composed of two group I ribozymes with different functions, separated by an open reading frame. Here, we show that DiSSU1 is mobile when haploid intron-containing and intron-less amoebae are mated. The mobility process is fast, being completed in 5-10 nuclear cycles after mating in the developing zygote and plasmodia. Analyses of progeny from genetic crosses confirm intron mobility. DiSSU1 is the first example of a mobile group I twintron. The intron-encoded protein was expressed in Escherichia coli and found to be an endonuclease, I-Dir I, that cleaves an intron-less ribosomal DNA allele at the intron-insertion site, and is probably involved in intron homing. The endonuclease I-Dir I seems to be a rare example of a protein that is expressed from a ribozyme-processed RNA polymerase I transcript in vivo.

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Non-mendelian inheritance of mitochondrial DNA and ribosomal DNA in the myxomycete, Didymium iridis

Cited by 24 publications

References 35 publications

Disappearance of mtDNA During Mating of the True Slime Mold Didymium iridis

Disappearance of mtDNA During Mating of the True Slime Mold Didymium iridis

Maternal inheritance of mitochondria: multipolarity, multiallelism and hierarchical transmission of mitochondrial DNA in the true slime mold Physarum polycephalum

In vivo mobility of a group I twintron in nuclear ribosomal DNA of the myxomycete Didymium iridis

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