Non-small-cell lung cancer and miRNAs: novel biomarkers and promising tools for treatment

Feng, Bing; Zhang, Kai; Wang, Rui; Chen, Longbang

doi:10.1042/cs20140530

Cited by 62 publications

(60 citation statements)

References 168 publications

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“…PTEN expression is decreased in patients with NSCLC, and its deficiency is linked to cancer development and progression [32,33]. Growing evidence has shown that PTEN inhibited tumor cell growth and invasion by blocking the PI3K/Akt pathway [27] since it can negatively regulate the activity of Akt pathway by dephosphorylating PIP3 at the 3-phosphate site [24]. In this present study, we further confirmed that PTEN is a direct target of miR-92a in NSCLC by luciferase reporter assays, qRT-PCR, and western blotting assay.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, increasing evidence has suggested that aberrant expression of miRNAs plays important roles in NSCLC occurrence and development [14,15]. Therefore, identification of NSCLC-specific miRNAs and their targets is critical for understanding their roles in NSCLC tumorigenesis, which may contribute to find out diagnosis marker and novel therapeutic targets for NSCLC [24]. In the current study, we observed that miR-92a was upregulated in NSCLC compared with adjacent non-cancerous tissues, and its expression was significantly correlated with clinicopathological features, including lymph node metastasis (P < 0.01), tumor size (P<0.01), and TNM stage (P<0.01).…”

Section: Discussionmentioning

confidence: 99%

“…At indicated time points (24,48, and 72 h), the culture medium was removed and replaced with culture medium containing 10 μl of sterile 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye (5 mg/ml). After incubation at 37°C for 4 h,it was followed by removal of the MTT solution and the addition of 150 μl dimethyl sulfoxide (DMSO, Sigma-Aldrich) to each well followed by measuring the absorbance at 570 nm on an enzyme immunoassay analyzer (Bio-Rad, USA).…”

Section: Mtt Assaymentioning

confidence: 99%

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MicroRNA-92a promotes growth, metastasis, and chemoresistance in non-small cell lung cancer cells by targeting PTEN

et al. 2015

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MicroRNA-92a (miR-92a) has been reported to play important roles in tumorigenesis of human various cancers. However, the roles and underlying molecular mechanism of miR-92a in non-small cell lung cancer (NSCLC) have not been totally elucidated. Therefore, the aims of this study were to determine the role of miR-92a and to elucidate its regulatory mechanism in NSCLC. We found that miR-92a was significantly upregulated in NSCLC tissues compared to matched adjacent normal lung tissues, and its expression is significantly associated with clinical characteristics of patients, including tumor, node, and metastasis (TNM) stage; tumor size; and lymph node metastasis (all P < 0.01). Function assays demonstrated that upregulation of miR-92a in NSCLC cells promoted cell proliferation, migration, and invasion, decreased apoptosis and caspase-3 activity, and enhanced chemoresistance of NSCLC cells, whereas downregulation of miR-92a showed the opposite effects. Moreover, phosphatase and tensin homolog (PTEN), a unique tumor suppressor gene, was confirmed as a direct target of miR-92a, and PTEN messenger RNA (mRNA) expression was decreased in NSCLC tissues and was inversely correlated with miR-92a. Downregulation of PTEN could mimic the same effects of miR-92a mimic in NSCLC cells and rescue the effects on NSCLC cells induced by miR-92a inhibitor. Taken together, these findings suggested that miR-92a could promote growth, metastasis, and chemoresistance in NSCLC cells at least partially by targeting PTEN.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Mtt Assaymentioning

confidence: 99%

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MicroRNA-92a promotes growth, metastasis, and chemoresistance in non-small cell lung cancer cells by targeting PTEN

et al. 2015

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“…miRNAs are involved in the pathogenesis of lung diseases, including lung cancer, cystic fibrosis, chronic obstructive pulmonary disease, asthma and idiopathic pulmonary fibrosis (12). miRNAs contribute to the development of novel diagnostic biomarkers and personalized therapeutic tools in NSCLC (13). miR-145 is silenced by DNA methylation and acts as a prognostic biomarker in NSCLC (14).…”

Section: Introductionmentioning

confidence: 99%

miR‑145‑5p inhibits epithelial‑mesenchymal transition via the JNK signaling pathway by targeting MAP3K1 in non‑small cell lung cancer cells

et al. 2017

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Abstract. Lung cancer is one of the most common types of tumors and the leading cause of cancer-associated mortality in the world. Additionally, non-small cell lung cancer (NSCLC) accounts for ~80% of all lung cancer cases. Epithelialmesenchymal transition (EMT) is an important cell biological process, which is associated with cancer migration, metastasis, asthma and fibrosis in the lung. In the present study, it was revealed that miR-145-5p was able to suppress EMT by inactivating the c-Jun N-terminal kinase (JNK) signaling pathway in NSCLC cells. Mitogen-activated protein kinase kinase kinase 1 (MAP3K1) was predicted and confirmed to be a novel target of miR-145-5p. Overexpression of MAP3K1 was able to reverse the inhibition of EMT induced by miR-145-5p via the JNK signaling pathway. Overall, the results revealed that miR-145-5p inhibits EMT via the JNK signaling pathway by targeting MAP3K1 in NSCLC cells.

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“…It has become increasingly evident that different miRNAs can play either oncogenic or tumor-suppressive roles in a wide variety of pathways, depending on the target genes or the cellular context (6,7). miR-200c is a member of the miR-200 family, which consists of five members (miR-200a, miR-200b and miR-429 comprise cluster 1, which is located on chromosome 1p36; and miR-200c and miR-141 comprise cluster 2, which is located on chromosome 12p13) (8).…”

Section: Introductionmentioning

confidence: 99%

Potential use of microRNA-200c as a prognostic marker in non-small cell lung cancer

Tian

Yue

et al. 2017

Oncology Letters

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Abstract. MicroRNAs (miRNAs/miRs) are a class of small, highly conserved non-coding RNAs that can serve either oncogenic or tumor-suppressive roles in a wide variety of tumors. miR-200c is a member of the miR-200 family whose specific role in non-small cell lung cancer (NSCLC) has not yet been elucidated. The purpose of the present study was to detect the expression level of miR-200c in NSCLC, and to analyze its association with clinicopathological factors and patient prognosis. The present study determined the expression levels of miR-200c in 110 tumor samples collected from patients diagnosed with NSCLC who underwent complete tumor resection with regional lymph node dissection, as assessed by reverse transcription-quantitative polymerase chain reaction. The association between the expression level of miR-200c and clinicopathological features and patient prognosis was also analyzed. The results showed that miR-200c overexpression was detected in 66 of the 110 cases and was significantly associated with positive lymph node metastasis (P<0.001). Univariate survival analysis demonstrated that high miR-200c expression, positive lymph node metastasis and advanced Tumor-Node-Metastasis (TNM) classification stage significantly predicted decreased 5-year disease-free survival rates (all P<0.05) and poor 5-year overall survival rates (all P<0.01), respectively. The results of multivariate Cox regression analysis showed that TNM stage and miR-200c expression retained its significance as an independent prognostic factor for unfavorable 5-year disease-free survival rates (P<0.05) and poor 5-year overall survival rates (P<0.01). The present findings suggest that miR-200c overexpression is significantly associated with poor survival rates in NSCLC and that miR-200c could play an oncogenic role. miR-200c may have clinical potential as a promising prognostic predictor for patients with NSCLC.

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Non-small-cell lung cancer and miRNAs: novel biomarkers and promising tools for treatment

Cited by 62 publications

References 168 publications

MicroRNA-92a promotes growth, metastasis, and chemoresistance in non-small cell lung cancer cells by targeting PTEN

MicroRNA-92a promotes growth, metastasis, and chemoresistance in non-small cell lung cancer cells by targeting PTEN

miR‑145‑5p inhibits epithelial‑mesenchymal transition via the JNK signaling pathway by targeting MAP3K1 in non‑small cell lung cancer cells

Potential use of microRNA-200c as a prognostic marker in non-small cell lung cancer

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