Non-surgical treatment for periodontitis and peri-implantitis: longitudinal clinical and bacteriological findings—A case report with a 7-year follow-up evaluation

Shiba, Takahiko; Watanabe, Takayasu; Komatsu, Keiji; Koyanagi, Tatsuro; Nemoto, Takashi; Ohsugi, Yujin; Michi, Yasuyuki; Katagiri, Seiji; Takeuchi, Yasuo; Ishihara, Kazuyuki; Iwata, Takanori

doi:10.1177/2050313x211029154

Cited by 7 publications

(7 citation statements)

References 22 publications

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“…Moreover, abundance alteration could happen to part of the 14 genera screened after the treatment of peri‐implant disease. The relative abundance of Tannerella and Porphyromonas decreased after nonsurgical treatment for peri‐implantitis, whereas the relative abundance of Rothia increased (Shiba et al, 2021). A study found Porphyromonas and Treponema could be influenced by peri‐implantitis treatment (Nie et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Mucosal bleeding correlates with submucosal microbial dysbiosis in peri‐implant mucositis of patients with periodontitis

Sun

Wei

et al. 2023

Clinical Oral Implants Res

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ObjectivesThis study aimed to investigate the relationship between microbial communities and the severity of peri‐implant mucosal bleeding in peri‐implant mucositis.Materials and MethodsSubmucosal plaque samples were collected from 54 implants divided into the healthy implant (HI) group, peri‐implant mucositis (PM) group, and peri‐implantitis (PI) group. Sequencing of 16S rRNA was performed using the Illumina MiSeq platform. Alpha diversity (i.e., Shannon and Chao index) and beta diversity were used to measure microbial diversity within and between microbial communities, respectively. Differences in microbial taxa between groups were assessed via linear discriminate analysis effect size. Correlation between the modified sulcus bleeding index (mSBI) and microbial dysbiosis index (MDI) was examined using Spearman correlation analysis and linear models.ResultsThe submucosal bacterial richness (Chao index) was positively correlated with the mean mSBI in the PM group. As the mean mSBI increased in the PM group, the beta diversity became closer to that of the PI group. In the PM group, the abundances of 47 genera were significantly correlated with the mean mSBI, and the MDI was positively associated with the mean mSBI. Fourteen of the forty‐seven genera were discriminative taxa between the HI and PI groups, and the abundances of these biomarkers became closer to those in the PI group in the progression of peri‐implant disease.ConclusionsA higher mSBI value corresponded to a higher risk of microbial dysbiosis in peri‐implant mucositis. The biomarkers identified may be useful for monitoring the progression of peri‐implant disease.

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Section: Discussionmentioning

confidence: 99%

Mucosal bleeding correlates with submucosal microbial dysbiosis in peri‐implant mucositis of patients with periodontitis

Sun

Wei

et al. 2023

Clinical Oral Implants Res

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“…Furthermore, the V4 region should be included in the primer design since the V4 region was the best sub-region for phylogenetic resolution among other 16S rRNA gene variable regions (Yang et al, 2016). In modern research for bacterial composition in the oral cavity, many reports using the V3-V4 region have been performed, and good results have been reported (Maruyama et al, 2014;Komatsu et al, 2020;Shiba et al, 2021;Shimogishi et al, 2021). In contrast, many studies have shown effective results using ancient microbiome through the amplicon sequencing of the V4 region (Santiago-Rodriguez et al, 2017;Santiago-Rodriguez et al, 2019) because ancient DNA is known to be highly fragmented (Sawyer et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Periodontal Bacteria of Edo and Modern Periods Using Novel Diagnostic Approach for Periodontitis With Micro-CT

Shiba

Komatsu

Sudo

et al. 2021

Front. Cell. Infect. Microbiol.

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Ancient dental calculus, formed from dental plaque, is a rich source of ancient DNA and can provide information regarding the food and oral microbiology at that time. Genomic analysis of dental calculus from Neanderthals has revealed the difference in bacterial composition of oral microbiome between Neanderthals and modern humans. There are few reports investigating whether the pathogenic bacteria of periodontitis, a polymicrobial disease induced in response to the accumulation of dental plaque, were different between ancient and modern humans. This study aimed to compare the bacterial composition of the oral microbiome in ancient and modern human samples and to investigate whether lifestyle differences depending on the era have altered the bacterial composition of the oral microbiome and the causative bacteria of periodontitis. Additionally, we introduce a novel diagnostic approach for periodontitis in ancient skeletons using micro-computed tomography. Ancient 16S rDNA sequences were obtained from 12 samples at the Unko-in site (18th-19th century) of the Edo era (1603–1867), a characteristic period in Japan when immigrants were not accepted. Furthermore, modern 16S rDNA data from 53 samples were obtained from a database to compare the modern and ancient microbiome. The microbial co-occurrence network was analyzed based on 16S rDNA read abundance. Eubacterium species, Mollicutes species, and Treponema socranskii were the core species in the Edo co-occurrence network. The co-occurrence relationship between Actinomyces oricola and Eggerthella lenta appeared to have played a key role in causing periodontitis in the Edo era. However, Porphyromonas gingivalis, Fusobacterium nucleatum subsp. vincentii, and Prevotella pleuritidis were the core and highly abundant species in the co-occurrence network of modern samples. These results suggest the possibility of differences in the pathogens causing periodontitis during different eras in history.

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“…The libraries were then sequenced on the MiSeq platform (Illumina, CA, USA) to obtain 2 × 300 bp paired-end reads. 17,18 The read data were submitted to the DNA Data Bank of Japan (http://www.ddbj.nig. ac.jp/) under accession number DRA012487.…”

Section: Results Of Bacteriological Examinationmentioning

confidence: 99%

“…High-throughput sequencing technology is ideal for the comprehensive evaluation of changes in bacterial composition in polymicrobial diseases prior to and post-treatment. 18 Here, we report two cases that had an apically positioned flap (APF) combined with IP and erbiumdoped yttrium aluminum garnet (Er:YAG) laser irradiation, before and after implantoplasty (IP), for peri-implantitis treatment; and with FGG to increase the KM and oral vestibule. The clinical and bacteriological effects of the treatments were evaluated longitudinally via high-throughput sequencing.…”

mentioning

confidence: 99%

Peri-implantitis management by resective surgery combined with implantoplasty and Er:YAG laser irradiation, accompanied by free gingival graft: a case report

Shiba

Komatsu

Watanabe

et al. 2023

Therapeutic Advances in Chronic Disease

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The optimal method for decontamination of implant surfaces for peri-implantitis treatment remains controversial. In recent years, erbium-doped yttrium aluminum garnet (Er:YAG) laser irradiation and implantoplasty (IP) (i.e. mechanical modification of the implant) have been reported to be effective in decontaminating implant surfaces during the surgical treatment. Also, a lack of adequate keratinized mucosa (KM) around the implant is known to be associated with more plaque accumulation, tissue inflammation, attachment loss, and mucosal recession, increasing the risk of peri-implantitis. Therefore, free gingival graft (FGG) has been recommended for gaining adequate KM around the implant. However, the necessity of acquiring KM for the treatment of peri-implantitis using FGG remains unclear. In this report, we applied the apically positioned flap (APF) as resective surgery for peri-implantitis treatment in conjunction with IP and Er:YAG laser irradiation to polish/clean the implant surface. Furthermore, FGG was conducted simultaneously to create additional KM, which increased the tissue stability and contributed to the positive results. The two patients were 64 and 63 years old with a history of periodontitis. The removal of granulation tissue and debridement of contaminated implant surfaces were performed with Er:YAG laser irradiation post flap elevation and then modified smooth surfaces mechanically using IP. Er:YAG laser irradiation was also utilized to remove the titanium particles. In addition, we performed FGG to increase the width of KM as a vestibuloplasty. Peri-implant tissue inflammation and progressive bone resorption were not observed, and both patients maintained good oral hygiene conditions until the 1-year follow-up appointment. Bacterial analysis via high-throughput sequencing revealed proportional decreases in bacteria associated with periodontitis ( Porphyromonas, Treponema, and Fusobacterium). To the best of our knowledge, this study is the first to describe peri-implantitis management and bacterial change before and after procedures by resective surgery combined with IP and Er:YAG laser irradiation for peri-implantitis treatment, accompanied by FGG for increasing KM around the implants.

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Non-surgical treatment for periodontitis and peri-implantitis: longitudinal clinical and bacteriological findings—A case report with a 7-year follow-up evaluation

Cited by 7 publications

References 22 publications

Mucosal bleeding correlates with submucosal microbial dysbiosis in peri‐implant mucositis of patients with periodontitis

Mucosal bleeding correlates with submucosal microbial dysbiosis in peri‐implant mucositis of patients with periodontitis

Comparison of Periodontal Bacteria of Edo and Modern Periods Using Novel Diagnostic Approach for Periodontitis With Micro-CT

Peri-implantitis management by resective surgery combined with implantoplasty and Er:YAG laser irradiation, accompanied by free gingival graft: a case report

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