Non-targeted sportomics analyses by mass spectrometry to understand exercise-induced metabolic stress in soccer players

Prado, Enrique A. Silveira; Souza, Gustavo H. M. F.; Pegurier, Marcelle; Vieira, Camila; Lima-Neto, Abelardo Barbosa Moreira; Assis, Marcio; Guedes, Maria Izabel Florindo; Koblitz, María Gabriela Bello; Ferreira, Mariana Simões Larraz; Macedo, Andrea Furtado; Bottino, Altamiro; Bassini, Adriana; Cameron, Luiz Cláudio

doi:10.1016/j.ijms.2017.02.002

Cited by 22 publications

(19 citation statements)

References 15 publications

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“…11). Generally, the concentrations of nucleotide degradation products increase most after highintensity exercise [63] and most concentration changes occur between > 0.5 and 3 h after exercise [31,43] (Figs. 4 and 11).…”

Section: After Exercise the Concentrations Of Nucleotide Degradationmentioning

confidence: 99%

Metabolite Concentration Changes in Humans After a Bout of Exercise: a Systematic Review of Exercise Metabolomics Studies

et al. 2020

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Background: Exercise changes the concentrations of many metabolites, which are small molecules (< 1.5 kDa) metabolized by the reactions of human metabolism. In recent years, especially mass spectrometry-based metabolomics methods have allowed researchers to measure up to hundreds of metabolites in a single sample in a non-biased fashion. To summarize human exercise metabolomics studies to date, we conducted a systematic review that reports the results of experiments that found metabolite concentrations changes after a bout of human endurance or resistance exercise.Methods: We carried out a systematic review following PRISMA guidelines and searched for human metabolomics studies that report metabolite concentrations before and within 24 h after endurance or resistance exercise in blood, urine, or sweat. We then displayed metabolites that significantly changed their concentration in at least two experiments. Results: Twenty-seven studies and 57 experiments matched our search criteria and were analyzed. Within these studies, 196 metabolites changed their concentration significantly within 24 h after exercise in at least two experiments. Human biofluids contain mainly unphosphorylated metabolites as the phosphorylation of metabolites such as ATP, glycolytic intermediates, or nucleotides traps these metabolites within cells. Lactate, pyruvate, TCA cycle intermediates, fatty acids, acylcarnitines, and ketone bodies all typically increase after exercise, whereas bile acids decrease. In contrast, the concentrations of proteinogenic and non-proteinogenic amino acids change in different directions.Conclusion: Across different exercise modes and in different subjects, exercise often consistently changes the average concentrations of metabolites that belong to energy metabolism and other branches of metabolism. This dataset is a useful resource for those that wish to study human exercise metabolism.

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Section: After Exercise the Concentrations Of Nucleotide Degradationmentioning

confidence: 99%

Metabolite Concentration Changes in Humans After a Bout of Exercise: a Systematic Review of Exercise Metabolomics Studies

et al. 2020

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“…More recently, Stander et al have shown that the serum concentrations of carbohydrates, fatty acids, TCA cycle intermediates, and ketones were increased after a marathon, while the levels of amino acids were reduced ( Stander et al, 2018 ). Prado et al even proposed the term “sportomics” to qualify the use of “-omics” sciences to better understand the metabolic changes induced by a physical activity ( Prado et al, 2017 ). They performed untargeted metabolomics in urine before and after a soccer match.…”

Section: Introductionmentioning

confidence: 99%

“…They performed untargeted metabolomics in urine before and after a soccer match. Different categories of metabolites were found to be interesting, among which are organic acids ( Prado et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Blood Microsampling to Monitor Metabolic Profiles During Physical Exercise

Nix

Hemmati

Cobraiville

et al. 2021

Front. Mol. Biosci.

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Monitoring approaches and technical improvements are key factors to improve a sportsman’s health, training, and recovery after an injury. In this study, a targeted metabolomics approach using microsampling with hemaPEN® was developed to measure changes in blood concentrations of nine amino acids and four organic acids before, during, and after exercise. The aim of this research project was to investigate if a reliable monitoring of metabolite levels during sports activity can be achieved by collecting one drop of whole blood at different time points. A hemaPEN device is an easy-to-use and noninvasive microsampling technique designed to collect four accurate and precise blood volumes simultaneously (10.96 µl). Twenty healthy volunteers between 19 and 30 years of age were included in this study. Physical activity consisted in running as fast as possible 1,600 m after 400 m warm-up. One drop of blood was collected at five time points: before exercise, after 800-m running, after 1,600 m, and 30 min and 60 min after finishing the exercise. The influence of physical activity on metabolite levels was evaluated using two ultrahigh-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) methods. Analytical performance criteria such as metabolite stability, method precision, trueness, and accuracy were found to be satisfactory. Expected significant metabolic changes were identified for lactic acid, main TCA cycle intermediates, and some amino acids (e.g., creatinine, choline, and taurine). This preliminary study performed on a small cohort demonstrated a high interest of using microsampling for fluxomics analysis, not only to collect quickly and easily biological samples during sports events but also because it is much easier to store and to process the samples than classical plasma/serum samples obtained by venipuncture. The present results open new avenue for fluxomics analysis in the context of health care.

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“…Proteomics consists of the application of technologies, mainly mass spectrometry, to study the expression of proteins in a given biological matrix in a specific physiological context. Currently, a hypothesis-free and non-targeted proteomics approach is used to understand metabolic responses to physical stress through the analysis of tissue samples, cell and biological extracts, biofluids such as blood, urine and sweat (Bongiovanni et al, 2015;Aslam et al, 2017;Prado et al, 2017). However, there are few published studies that have analyzed salivary proteomics to assess physical activity, although the analytical potential of this fluid has been demonstrated in previous studies (Zauber et al, 2016;Franco-Martínez et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Análise de proteínas salivares de corredores de rua após evento esportivo real

Amaral

Ponte

Martins

et al. 2021

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The aim was to characterize acute exercise-induced changes in salivary proteins of street runners. Saliva samples from 12 adult male athletes were collected before and immediately after a street race. Two groups were formed based on the distance covered, 5 km (n=4) and 10 km (n=8). Samples were subjected to depletion of amylase, albumin and immunoglobulin G. Then they were concentrated, digested and analyzed by nano-UPLC-tandem nano-ESI-MSE. A total of 69 proteins were identified. Significant changes were observed in the expression of 15 proteins in the 5 km group and 13 proteins in the 10 km group. Among the proteins with altered expression, only 7 had already been described in the literature in similar models (Alpha-Amylase 1, Lactoperoxidase, Alpha Skeletal Muscle Actin, Cystatin-B, Cystatin- SN, Cystatin- SA and Androgen-regulated Protein 3B in the submaxillary gland). This study has shown that street running induces acute changes in the salivary proteome. The results obtained add to the limited data available in the literature in the search for a better understanding of the acute effects of exercise.

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Non-targeted sportomics analyses by mass spectrometry to understand exercise-induced metabolic stress in soccer players

Cited by 22 publications

References 15 publications

Metabolite Concentration Changes in Humans After a Bout of Exercise: a Systematic Review of Exercise Metabolomics Studies

Metabolite Concentration Changes in Humans After a Bout of Exercise: a Systematic Review of Exercise Metabolomics Studies

Blood Microsampling to Monitor Metabolic Profiles During Physical Exercise

Análise de proteínas salivares de corredores de rua após evento esportivo real

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