A retrospective study was conducted to identify the prevalence of COVID-19 through serology and RT-PCR in children, adolescents and adults. A database of the COVID-19 Tracking Program in school children was used. Methods: The data comprised sociodemographic and clinical variables, results of serological tests (IgM and IgG), and real-time-polymerase chain reaction (RT-PCR) results of IgM-positive individuals. The statistical analysis was performed with a 5% significance level. Results: Among 423 children, 107 (25.3%) exhibited seroprevalence with IgG, IgM or IgG/IgM; among 854 adolescents, 250 (29.2%) had positive serology; and among 282 adults, 59 (20.9%) were positive.The frequency of positivity on RT-PCR for SARS-CoV-2 was 3.5%, 3.6% and 6.0% in children, adolescents and adults, respectively. Children had a lower incidence of symptoms than adolescents (p = 0.001) and adults (p = 0.003); the most frequent were fever, ageusia, anosmia, headache, dry cough, sore throat, muscle pain, runny nose, dyspnoea, and diarrhoea. Conclusions: The prevalence rate for all groups was 26.7% in serology and 4.04% in RT-PCR. Children had lower rates of IgM and fewer symptoms compared with adolescents and adults. The data suggest the potential for transmissibility in all age groups.

Ciência. 2. Ciências da vida-Pesquisa-Brasil. I. Pereira, Denise. II. Série. CDD 570.9 Elaborado por Maurício Amormino Júnior-CRB6/2422 O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. 2019 Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Most cases of Hepatitis C are asymptomatic and symptoms appear when the disease is already hepatic. The present study aimed at the production and purification of the Hepatitis C virus core protein (HCV) in the prokaryotes system and its subcloning in eukaryonite expression vectors. The E. coli bacterium was selected to perform the synthesis of the protein of interest, which was purified by affinity chromatography and detected by western blotting. In addition, the sequence of interest was recombined in plasmids for targeting to plant cell sub-compartments, which were confirmed by PCR. The processes performed were successful and the subsequent expression in the different cell compartments will allow the best performance for the production of immunogens and enable the production of diagnostic kit.

No abstract