Noncoding RNAs as Biomarkers for Acute Coronary Syndrome

Wang, Lijie; Jin, Yinji

doi:10.1155/2020/3298696

“…The sponge adsorption effect of circRNA on miRNA occurs mainly through response elements binding miRNA to block the inhibition of miRNA on its target gene expression. When circRNA is highly expressed, the target gene expression of miRNA is up‐regulated, and when circRNA is lowly expressed, the target gene expression of miRNA is down‐regulated 22 . It can be said that the miRNA sponge function performed by circRNA plays an important role in the occurrence of diseases, competitively inhibiting the expression of miRNA and blocking the expression of miRNA on target genes.…”

Section: Discussionmentioning

confidence: 99%

Research on the circular RNA bioinformatics in patients with acute myocardial infarction

Yin

¹

,

Tang

²

,

Jiang

³

2020

Clinical Laboratory Analysis

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Objective Through the detection of circular RNA (circRNA) using expression profiling chips, we searched for circRNAs related to acute myocardial infarction (AMI) and explored their relationship and possible mechanisms with AMI. Method The study subjects included 3 AMI patients and 3 controls, and circRNA expression profiling analysis was performed using a microarray gene chip to identify circRNAs with large differences in expression between groups and to construct a circRNA‐miRNA network. Results Compared with the control group, there were 650 differentially expressed circRNAs found in AMI patients (P < .05, fold change > 2), including 535 up‐regulated circRNAs, such as hsa_circ_0050908, hsa_circRNA4010‐22, hsa_circ_0081241, hsa_circ_0010551, hsa_circRNA4010‐20, hsa_circRNA14702, hsa_circ_0115392, has_circRNA1825‐44, has_circRNA8493‐7, and hsa_circ_0025097. Furthermore, there were 115 down‐regulated circRNAs, such as hsa_circ_0066439, hsa_circ_0054211, hsa_circ_0095920, hsa_circ_0122984, hsa_circ_0113067, hsa_circ_0039155, hsa_circRNA4014‐45, hsa_circ_0122979, hsa_circ_0059665, and hsa_circ_0009319. The circRNAs hsa_circ_0066439, hsa_circ_0081241, and hsa_circ_0122984 can regulate multiple signal pathways to participate in the AMI process through hsa‐miR‐1254, hsa‐miR‐328‐5p, and other miRNAs. In addition, the expression of circRNA‐miRNA in peripheral blood is related to the network. Differentially expressed circRNAs are involved in chromatin organization, chromatin‐modifying enzymes, signal transduction, lysine degradation, the mitogen‐activated protein kinase (MAPK) signaling pathway, focal adhesion, and a variety of other pathways, such as myocardial infarction, coronary heart disease, hypertension, and other diseases. The gene ontology analysis results show that molecular function mainly involves binding and molecular structural activity, whereas the biological process mainly involves a single biological process, a cellular component for organization, and a cellular process, and the cellular component mainly involves a protein complex, an extracellular matrix, and a membrane. Conclusion circRNA and microRNA interact to participate in the development of AMI. circRNA may be involved in the pathogenesis of AMI.

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“…Circulating lncRNAs have been considered as diagnosed biomarkers for CAD [27]. Herein, two datasets GSE113079 and GSE69587 were collected for screening abnormally expressed circulating lncRNAs for CAD.…”

Section: Abnormally Expressed Circulating Lncrnas For Cadmentioning

confidence: 99%

[Retracted] Dysregulated Circulating Apoptosis‐ and Autophagy‐Related lncRNAs as Diagnostic Markers in Coronary Artery Disease

Zhang

¹

,

Lou

²

,

He

³

et al. 2021

BioMed Research International

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Objective. Increasing evidence emphasizes the implications of dysregulated apoptosis and autophagy cellular processes in coronary artery disease (CAD). Herein, we aimed to explore apoptosis- and autophagy-related long noncoding RNAs (lncRNAs) in peripheral blood of CAD patients. Methods. The mRNA and lncRNA expression profiles were retrieved from the Gene Expression Omnibus (GEO) database. With ∣ fold change ∣ > 1.5 and adjusted p value < 0.05, differentially expressed apoptosis- and autophagy-related mRNAs were screened between CAD and healthy blood samples. Also, differentially expressed lncRNAs were identified for CAD. Using the psych package, apoptosis- and autophagy-related lncRNAs were defined with Spearson’s correlation analysis. Receiver operating characteristic (ROC) curves were conducted for the assessment of the diagnosed efficacy of these apoptosis- and autophagy-related lncRNAs. Results. Our results showed that 24 apoptosis- and autophagy-related mRNAs were abnormally expressed in CAD than normal controls. 12 circulating upregulated and 1 downregulated apoptosis- and autophagy-related lncRNAs were identified for CAD. The ROCs confirmed that AC004485.3 ( AUC = 0.899 ), AC004920.3 ( AUC = 0.93 ), AJ006998.2 ( AUC = 0.776 ), H19 ( AUC = 0.943 ), RP5-902P8.10 ( AUC = 0.956 ), RP5-1114G22.2 ( AUC = 0.883 ), RP11-247A12.1 ( AUC = 0.885 ), RP11-288L9.4 ( AUC = 0.928 ), RP11-344B5.2 ( AUC = 0.858 ), RP11-452C8.1 ( AUC = 0.929 ), RP11-565A3.1 ( AUC = 0.893 ), and XXbac-B33L19.4 ( AUC = 0.932 ) exhibited good performance in differentiating CAD from healthy controls. Conclusion. Collectively, our findings proposed that circulating apoptosis- and autophagy-related lncRNAs could become underlying diagnostic markers for CAD in clinical practice.

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“…Acute coronary syndrome (ACS) is the most fatal form of ischemic heart disease, including ST-segment elevation myocardial infarction (STEMI), non-STEMI, and unstable angina ( Falk et al, 2013 ). The main clinical presentations in ACS consist of acute myocardial infarction, unstable angina ( Wang and Jin, 2020 ), chest pain, sweating, and nausea ( Khan et al, 2013 ). Advanced atherosclerosis with thrombus is one of the main characteristics of ACS ( Libby et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

microRNA-9 Inhibits Vulnerable Plaque Formation and Vascular Remodeling via Suppression of the SDC2-Dependent FAK/ERK Signaling Pathway in Mice With Atherosclerosis

Zhang

¹

,

Song

²

,

Hong

³

et al. 2020

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microRNAs (miRNAs or miRs) play important roles in modulating the occurrence and progression of atherosclerosis and acute coronary syndrome (ACS). Herein, this study aimed to investigate the possible role of miR-9 in the development of atherosclerosis. Initially, the differentially expressed genes associated with ACS were screened and miRNAs that regulate syndecan-2 (SDC2) were predicted using microarray analysis. Furthermore, the biological functions of miR-9 and SDC2 on aortic plaque area, proliferation of collagen fibers, Mac-3-labeled macrophages, inflammatory response, and levels of the focal adhesion kinase/extracellular signal-regulated kinase (FAK/ERK) signaling pathway-related proteins in atherosclerosis were evaluated after ectopic miR-9 expression or SDC2 depletion in ACS mice using oil red O staining, Masson’s trichrome staining, immunohistochemistry, and Western blot analysis, respectively. SDC2 was highly-expressed, while miR-9 was poorly-expressed in atherosclerosis. Additionally, miR-9 targeted SDC2 and negatively-regulated its expression. Up-regulation of miR-9 reduced aortic plaque area, the proliferation of collagen fibers, Mac-3-labeled macrophages and levels of IL-6, IL-1β, and TNF-α by suppressing SDC2 and the FAK/ERK signaling pathway, thereby ameliorating atherosclerosis in ACS mice. In conclusion, the current study provides evidence that miR-9 retards atherosclerosis by repressing SDC2 and the FAK/ERK signaling pathway, highlighting a new theoretical basis for the treatment of atherosclerosis.

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Noncoding RNAs as Biomarkers for Acute Coronary Syndrome

Cited by 24 publications

References 105 publications

Research on the circular RNA bioinformatics in patients with acute myocardial infarction

Research on the circular RNA bioinformatics in patients with acute myocardial infarction

[Retracted] Dysregulated Circulating Apoptosis‐ and Autophagy‐Related lncRNAs as Diagnostic Markers in Coronary Artery Disease

microRNA-9 Inhibits Vulnerable Plaque Formation and Vascular Remodeling via Suppression of the SDC2-Dependent FAK/ERK Signaling Pathway in Mice With Atherosclerosis

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