Nonenzymatic Detection of Bacterial Genomic DNA Using the Bio Bar Code Assay

Hill, Haley D.; Vega, Rafael A.; Mirkin, Chad A.

doi:10.1021/ac701626y

Cited by 124 publications

(74 citation statements)

References 42 publications

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“…At pH 7.0, the linear range of the H 2 O 2 sensor was from 0.5 to 115 mm, with a correlation coefficient of 0.9988 (inset in Figure 7), which is much wider than that (from 5 to 45 mm) of a reported biosensor. [40] The detection limit with a signal-to-noise ratio of three was estimated to be 0.12 mm, which is lower than the level of 1.4 mm reported for a (PDDA/Fe 3 O 4 NPs) 5 multilayer modified electrode. [41] For comparison, the amperometric responses of l-HCNTs/GCE and MWCNTs/ GCE are shown in Figure 7(curves b and c).…”

Section: More Importantly We Compared the Catalytic Efficiencies Of mentioning

confidence: 59%

Helical Carbon Nanotubes: Intrinsic Peroxidase Catalytic Activity and Its Application for Biocatalysis and Biosensing

Cui

Han

Zhu

2011

Chemistry A European J

188

118

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A combined hydrothermal/hydrogen reduction method has been developed for the mass production of helical carbon nanotubes (HCNTs) by the pyrolysis of acetylene at 475 °C in the presence of Fe(3)O(4) nanoparticles. The synthesized HCNTs have been characterized by high-resolution transmission electron microscopy, scanning electron microscopy, X-ray diffraction analysis, vibrating sample magnetometry, and contact-angle measurements. The as-prepared helical-structured carbon nanotubes have a large specific surface area and high peroxidase-like activity. Catalysis was found to follow Michaelis-Menten kinetics and the HCNTs showed strong affinity for both H(2)O(2) and 3,3',5,5',-tetramethylbenzidine (TMB). Based on the high activity, the HCNTs were firstly used to develop a biocatalyst and amperometric sensor. At pH 7.0, the constructed amperometric sensor showed a linear range for the detection of H(2)O(2) from 0.5 to 115 μM with a correlation coefficient of 0.999 without the need for an electron-transfer mediator. Because of their low cost and high stability, these novel metallic HCNTs represent a promising candidate as mimetic enzymes and may find a wide range of new applications, such as in biocatalysis, immunoassay, and environmental monitoring.

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Section: More Importantly We Compared the Catalytic Efficiencies Of mentioning

confidence: 59%

Helical Carbon Nanotubes: Intrinsic Peroxidase Catalytic Activity and Its Application for Biocatalysis and Biosensing

Cui

Han

Zhu

2011

Chemistry A European J

188

118

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“…Protein-inorganic surface interactions have gained increasing attention owing to their widespread occurrence in nature, and their broad range of applications in nanobiotechnology (Choi et al 2009;Hill et al 2007;Hu et al 2005;Jackson et al 2000;Laera et al 2011;Manecka et al 2014;Millo et al 2009;Park et al 2008;Qin et al 2007a, b;Slocik et al 2011;Xu et al 2010). Adhesion of proteins on solid substrates is utilized by many organisms, e.g.…”

Section: Introductionmentioning

confidence: 99%

Modeling and simulation of protein–surface interactions: achievements and challenges

Ozboyaci

Kokh

Corni

et al. 2016

Quart. Rev. Biophys.

197

199

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Abstract. Understanding protein-inorganic surface interactions is central to the rational design of new tools in biomaterial sciences, nanobiotechnology and nanomedicine. Although a significant amount of experimental research on protein adsorption onto solid substrates has been reported, many aspects of the recognition and interaction mechanisms of biomolecules and inorganic surfaces are still unclear. Theoretical modeling and simulations provide complementary approaches for experimental studies, and they have been applied for exploring protein-surface binding mechanisms, the determinants of binding specificity towards different surfaces, as well as the thermodynamics and kinetics of adsorption. Although the general computational approaches employed to study the dynamics of proteins and materials are similar, the models and force-fields (FFs) used for describing the physical properties and interactions of material surfaces and biological molecules differ. In particular, FF and water models designed for use in biomolecular simulations are often not directly transferable to surface simulations and vice versa. The adsorption events span a wide range of time-and length-scales that vary from nanoseconds to days, and from nanometers to micrometers, respectively, rendering the use of multi-scale approaches unavoidable. Further, changes in the atomic structure of material surfaces that can lead to surface reconstruction, and in the structure of proteins that can result in complete denaturation of the adsorbed molecules, can create many intermediate structural and energetic states that complicate sampling. In this review, we address the challenges posed to theoretical and computational methods in achieving accurate descriptions of the physical, chemical and mechanical properties of protein-surface systems. In this context, we discuss the applicability of different modeling and simulation techniques ranging from quantum mechanics through all-atom molecular mechanics to coarse-grained approaches. We examine uses of different sampling methods, as well as free energy calculations. Furthermore, we review computational studies of protein-surface interactions and discuss the successes and limitations of current approaches.

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“…As shown in Table 1, it was found that the K m value of CuFe 2 o 4 /gQDs with TMB as substrate was 3.3 times lower than that of HRP reported in the previous paper by comparing the apparent kinetic parameters [52], indicating that CuFe 2 o 4 / GQDs have a higher reactivity to TMB than HRP. Additionally, the K m value of CuFe 2 o 4 /gQDs with H 2 o 2 as substrate was 2 times lower than that of HRP and the other peroxides nanomimetics reported recently [53], suggesting that CuFe 2 o 4 /gQDs have a higher reactivity to H 2 o 2 than HRP and other nanomaterials. This might be because of the advantages of CuFe 2 o 4 /gQDs globular structures and quantum properties such as the larger specific surface area, stronger absortion ability, higher conductivity and dispersion for TMB and H 2 o 2 as substrates, which would provided a lot of practice sites of reaction and expanded contact areas of reaction, effectively improving the efficiency of the reaction.…”

Section: Kinetic Assay Of the Cufe2o4/gqds Mnpsmentioning

confidence: 56%

A Microfluidic Colorimetric Biosensor for Chlorpyrifos Determination based on Peroxidase-like CuFe2O4/GQDs Magnetic Nanoparticles

Mao¹,

Zuo²,

Ya³

2017

JRST

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High-precision and miniature biosensors are in urgent demand with respect to the organophosphorus pesticides used extensively in agriculture, especially for Chlorpyrifos. In this study, the novel CuFe 2 O 4 -Graphene Quantum Dots (CuFe 2 O 4 / GQDs) Magnetic Nano-Particles (MNPs) was prepared to amplify color signal as peroxidase mimetic using one-step hydrothermal method with electrostatic adsorption. Further combined with well-designed microfluidic chip containing enzyme inhibition reaction, color reaction and uV spectrophotometric detection areas, a colorimetric biosensor was developed based on CuFe 2 O 4 /GQDs for Chlorpyrifos determination. Experimental results of the biosensor displayed a linear range from 3.2 × 10 -8 M to 6 × 10 -7 M with a correlation coefficient of 0.99258 under the optimized conditions, and the Limit of Detection (LOD) of 1.07 × 10 -8 M based on S/N = 3 and the sensitivity of 855.26 (absorbance) per 1 mM. Great advantages have been exhibted in the Microfluidic colorimetric biosensor due to the small volume, and high precision for determination of low concentrations of organophosphorus pesticides, largely simplifying the tedious operation and lowing LOD, which is great benefit to the further development of low-cost, portable and high-precision biosensors.

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Nonenzymatic Detection of Bacterial Genomic DNA Using the Bio Bar Code Assay

Cited by 124 publications

References 42 publications

Helical Carbon Nanotubes: Intrinsic Peroxidase Catalytic Activity and Its Application for Biocatalysis and Biosensing

Helical Carbon Nanotubes: Intrinsic Peroxidase Catalytic Activity and Its Application for Biocatalysis and Biosensing

Modeling and simulation of protein–surface interactions: achievements and challenges

A Microfluidic Colorimetric Biosensor for Chlorpyrifos Determination based on Peroxidase-like CuFe2O4/GQDs Magnetic Nanoparticles

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