Nonenzymatically Glucosylated Serum Proteins in Patients With End-Stage Renal Disease

Haley, Roger J.; Ward, David M.

doi:10.1016/s0272-6386(86)80122-6

Cited by 13 publications

(3 citation statements)

References 38 publications

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“…Some of them found normal glycosylation values in uremic patients when using the TBA or affinity-chromatographic meth ods [19,25,31,33,34], but others like Haley and Ward [35] reported elevated levels of PGP determined with the TBA assay in hemodialysis and continuous peritoneal dialysis patients, while Bannon et al [36] also found 22% high GHb levels determined with the affinity chro matography method in hemodialysis patients and a dif ference in the GHb variances as compared with the nor mal group. These discrepancies are probably based on methodological differences: most works include few pa tients on conservative treatment, the range of renal func tion varies considerably among them, there are differ ences in the degree of anemia and blood transfusions received.…”

Section: Discussionmentioning

confidence: 99%

Nonenzymatic Glycosylation of Hemoglobin and Total Plasmatic Proteins in End-Stage Renal Disease

et al. 1991

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In order to ascertain whether there are abnormalities of nonenzymatic glycosylation in uremia, the levels of nonenzymatically glycosylated hemoglobin (GHb), and total plasmatic glycosylated proteins (PGP) were studied using the thiobarbituric acid (TBA) method, a procedure not interfered with by carbamylation. Total hemoglobin A₁ (HbA₁) and the A_1c fraction were also determined by ion exchange chromatographic methods. Sixty-six end-stage renal disease patients (29 nondiabetic and 8 diabetic uremic patients on conservative treatment, 29 non-diabetic hemodialysis patients) and 56 controls (32 nonuremic diabetic patients and 24 healthy controls) were studied. High levels of GHb and total PGP were found in the nondiabetic uremic group on conservative treatment with all the methods used, but the persistence of high chromatographically determined HbA₁ levels in hemodialysis patients contrasts with the results obtained with the other techniques, which showed lower values on hemodialysis. Nondiabetic uremic patients with abnormal oral glucose tolerance curves had significantly higher levels of TBA-determined GHb and PGP. Uremic diabetic patients had the highest glycosylation levels of all the studied groups. We conclude that there is an abnormal nonenzymatic glycosylation of proteins in uremia, independent of carbamylation reactions and partially corrected by hemodialysis.

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Section: Discussionmentioning

confidence: 99%

Nonenzymatic Glycosylation of Hemoglobin and Total Plasmatic Proteins in End-Stage Renal Disease

et al. 1991

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“…In particular, the fragmentation reactions exhibited by the compound appeared to us particularly appealing for investigation since the structure of the allantoin ring, with two sequential -C(]O)-N(H)-fragments, looked a good candidate to act as a precursor of isocyanic acid, a well known biologically pernicious substance that can easily react with amino terminus residues of proteins (or side chains of lysine and arginine residues) to form carbamoylated proteins, which have been observed in several states of disease. [12][13][14][15] Indeed, 1-phenyl-tetrazolone, which has structural similarities to allantoin possessing a single -C(]O)-N(H)-fragment in its heterocyclic ring has been recently shown to decompose easily to phenylazide and isocyanic acid. 16 In spite of both its many applications and expected interesting molecular properties and reactivity, allantoin has not yet been much studied from the structural point of view, though many publications describe procedures for its analytical determination, in particular in a biochemical context.…”

Section: Introductionmentioning

confidence: 99%

“…21 Kahn and Tipton 22 performed simple HF/6-31G(d,p) calculations on the isolated molecule of allantoin and reported the existence of four different conformers, with the form similar to the monomeric structure found in the RS-allantoin crystal being the second most stable one. According to the HF/6-31G(d,p) calculations, the most stable conformer (by 12.8 kJ mol À1 ) has also a cis C 4 -N 10 -C 11 -O 14 axis, but in this case the ureido group is directed toward the outside part of the molecule. Two additional conformers of higher energy bearing a trans C 4 -N 10 -C 11 -O 14 fragment were also described by Kahn and Tipton.…”

Section: Introductionmentioning

confidence: 99%

Thermal decomposition of allantoin as probed by matrix isolation FTIR spectroscopy

2009

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Low temperature matrix isolation and room temperature crystalline state IR spectra Thermal decomposition a b s t r a c tThe optimized geometries, energies of the possible conformers of allantoin (2,5-dioxo-4-imidazolidinyl urea, the diureide of glyoxylic acid) as well as the barriers for conformational interconversion have been calculated using the density functional theory [DFT(B3LYP)/6-311þþG(d,p)] method. The calculations predicted the existence of four conformers (gC, tT, g 0 C, and g 0 T; where the first and second symbols in the name of the conformers designate the conformation around the exocyclic NHC-NHCO and CNH-CO axes, respectively), with the gC form contributing to more than 98% of the population in gas phase at room temperature. This conformer is different from that corresponding to the monomeric unit found in crystalline RS-allantoin (g 0 C; Mootz, D. Acta Crystallogr. 1965, 19, 726), stressing the importance of intermolecular H-bonding in determining the structure of the crystal. Upon sublimation under vacuum (10 À6 mbar), the compound was found to undergo extensive decomposition to urea, isocyanic acid, NH 3 , and carbon. The identification of the decomposition products was made by using matrix isolation infrared spectroscopy. In consonance with the theoretical predictions, the allantoin molecules surviving thermal decomposition were found to undergo conformational isomerization and be present in the cryogenic argon matrix in both the gC and g 0 C conformations. The solid state room temperature infrared spectrum of allantoin was also investigated and assigned.

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Uremic Toxins in Chronic Renal Failure

Glorieux

Schepers

Vanholder

Calcium and Phosphate Metabolism Management in Chronic Renal Disease

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Nonenzymatically Glucosylated Serum Proteins in Patients With End-Stage Renal Disease

Cited by 13 publications

References 38 publications

Nonenzymatic Glycosylation of Hemoglobin and Total Plasmatic Proteins in End-Stage Renal Disease

Nonenzymatic Glycosylation of Hemoglobin and Total Plasmatic Proteins in End-Stage Renal Disease

Thermal decomposition of allantoin as probed by matrix isolation FTIR spectroscopy

Uremic Toxins in Chronic Renal Failure

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