Nonimmunogenetic Viral Capsid Carrier with Cancer Targeting Activity

Lee, Bo Ram; Jo, Eunji; Yoon, Hong Yeol; Yoon, Chul Joo; Lee, Hyo‐Jung; Kwon, Koo Chul; Kim, Tae Woo; Lee, Jeewon

doi:10.1002/advs.201800494

Cited by 10 publications

(5 citation statements)

References 46 publications

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“…For comparison, the Cy5.5 alone has previously been shown to have limited accumulation to the tumor area. 35 – 37 We also detected liver and kidney accumulation of the nano-complexes in the early time points, which diminished over time. At the 24 h time point, the fluorescence signal from the liver had greatly diminished compared to the tumors, while the kidneys maintained a strong signal, as potentially the nano-complexes were excreted through this organ.…”

Section: Discussionmentioning

confidence: 83%

<p>Nucleic Acid Delivery with α-Tocopherol-Polyethyleneimine-Polyethylene Glycol Nanocarrier System</p>

Hossian

Jois

Jonnalagadda

et al. 2020

IJN

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Purpose Nucleic acid-based therapies are a promising therapeutic tool. The major obstacle in their clinical translation is their efficient delivery to the desired tissue. We developed a novel nanosized delivery system composed of conjugates of α-tocopherol, polyethyleneimine, and polyethylene glycol (TPP) to deliver nucleic acids. Methods We synthesized a panel of TPP molecules using different molecular weights of PEG and PEI and analyzed with various analytical approaches. The optimized version of TPP (TPP 111 - the 1:1:1 molecular ratio) was self-assembled in water to produce nanostructures and then evaluated in diversified in vitro and in vivo studies. Results Through a panel of synthesized molecules, TPP 111 conjugate components self-assembled in water, forming globular shaped nanostructures of ~90 nm, with high nucleic acid entrapment efficiency. The polymer had low cytotoxicity in vitro and protected nucleic acids from nucleases. Using a luciferase-expressing plasmid, TPP 111 -plasmid nano-complexes were rapidly up-taken by cancer cells in vitro and induced strong transfection, comparable to PEI. Colocalization of the nano-complexes and endosomes/lysosomes suggested an endosome-mediated uptake. Using a subcutaneous tumor model, intravenously injected nano-complexes preferentially accumulated to the tumor area over 24 h. Conclusion These results indicate that we successfully synthesized the TPP 111 nanocarrier system, which can deliver nucleic acids in vitro and in vivo and merits further evaluation.

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Section: Discussionmentioning

confidence: 83%

<p>Nucleic Acid Delivery with α-Tocopherol-Polyethyleneimine-Polyethylene Glycol Nanocarrier System</p>

Hossian

Jois

Jonnalagadda

et al. 2020

IJN

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“…Samples of 10 mg mL −1 Dps in PBS, 10 mg mL −1 Dps‐C 3 in PBS containing 10 m m DTT, and 10 mg mL −1 BSA in PBS were used to measure the hydrodynamic size. To investigate the possible binding of Dps or Dps‐C 3 with BSA, [ 30 ] 0.2 mg mL −1 Dps or Dps‐C 3 in PBS containing 10 m m DTT were incubated with 1.5 mg mL −1 BSA at 37 °C for 30 min, and then dialyzed against PBS. After incubation for 18 or 36 h, the particle sizes of the mixtures were analyzed.…”

Section: Methodsmentioning

confidence: 99%

Effects of Individual Amino Acids on the Blood Circulation of Biosynthetic Protein Nanocages: Toward Guidance on Surface Engineering

Zhou

Zhang

et al. 2023

Adv Healthcare Materials

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Protein nanocages (PNCs) hold great promise for developing multifunctional nanomedicines. Long blood circulation is a key requirement of PNCs for most in vivo application scenarios. In addition to the classical PEGylation strategy, short peptides with a specific sequence screened via phage display are also very effective in prolonging the blood half‐life (t1/2) of PNCs. However, there is a lack of knowledge on how individual amino acids affect the circulation of PNCs. Here the effects of the 20 proteinogenic amino acids in the form of an X3 or X5 tag (X represents an amino acid) are explored on the pharmacokinetics of PNCs, which lead to the formation of a heatmap illustrating the extent of t1/2 prolongation by each proteinogenic amino acid. Significantly, oligo‐lysine and oligo‐arginine can effectively prolong the t1/2 of strongly negatively charged PNCs through charge neutralization, while oligo‐cysteine can also do so, but via a different mechanism, mediating the covalent binding of PNCs with plasma albumin as a stealth material. These findings are extendible and offer guidance for surface‐engineering biosynthetic PNCs and other nanoparticles.

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“…The subsequent crosslinking resulted in the formation of stable histone-HA NPs. The chemical modification by thiol groups and further co-assembly with HA could alter the natural structure of histone, providing the possibility to overcome or reduce the immunogenicity of histone [25]. As shown in Figure 1A, the mean hydrodynamic diameter of the histone-HA NPs was found at 195.4 nm measured by dynamic light scattering (DLS).…”

Section: Assembly and Characterization Of Histone-ha Nps And Sirna-ce...mentioning

confidence: 99%

Supramolecular Nanoparticles of Histone and Hyaluronic Acid for Co-Delivery of siRNA and Photosensitizer In Vitro

Hu,

Bao,

Zhang

et al. 2024

IJMS

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Small interfering RNA (siRNA) has significant potential as a treatment for cancer by targeting specific genes or molecular pathways involved in cancer development and progression. The addition of siRNA to other therapeutic strategies, like photodynamic therapy (PDT), can enhance the anticancer effects, providing synergistic benefits. Nevertheless, the effective delivery of siRNA into target cells remains an obstacle in cancer therapy. Herein, supramolecular nanoparticles were fabricated via the co-assembly of natural histone and hyaluronic acid for the co-delivery of HMGB1-siRNA and the photosensitizer chlorin e6 (Ce6) into the MCF-7 cell. The produced siRNA-Ce6 nanoparticles (siRNA-Ce6 NPs) have a spherical morphology and exhibit uniform distribution. In vitro experiments demonstrate that the siRNA-Ce6 NPs display good biocompatibility, enhanced cellular uptake, and improved cytotoxicity. These outcomes indicate that the nanoparticles constructed by the co-assembly of histone and hyaluronic acid hold enormous promise as a means of siRNA and photosensitizer co-delivery towards synergetic therapy.

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Nonimmunogenetic Viral Capsid Carrier with Cancer Targeting Activity

Cited by 10 publications

References 46 publications

<p>Nucleic Acid Delivery with α-Tocopherol-Polyethyleneimine-Polyethylene Glycol Nanocarrier System</p>

<p>Nucleic Acid Delivery with α-Tocopherol-Polyethyleneimine-Polyethylene Glycol Nanocarrier System</p>

Effects of Individual Amino Acids on the Blood Circulation of Biosynthetic Protein Nanocages: Toward Guidance on Surface Engineering

Supramolecular Nanoparticles of Histone and Hyaluronic Acid for Co-Delivery of siRNA and Photosensitizer In Vitro

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