Noninvasive assays of in vitro matured human oocytes showed insignificant correlation with fertilization and embryo development

Ashourzadeh, Sareh; Khalili, Mohammad Ali; Omidi, Mansoor; Nematollahi-Mahani, Seyed Noureddin; Kalantar, Seyed Mehdi; Aflatoonian, Abbas; Habibzadeh, Victoria

doi:10.1007/s00404-015-3644-y

Cited by 12 publications

(12 citation statements)

References 23 publications

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“…Despite optimizing the COH protocols, nearly 20% of the retrieved oocytes remain immature at the GV or MI stages (Ashourzadeh et al , 2015). Such immature eggs are considered unsuitable for clinical use and they are commonly discarded from the IVF programme (Escrich et al , 2011).…”

Section: Discussionmentioning

confidence: 99%

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Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development?

Faramarzi

Khalili

Ashourzadeh

et al. 2018

Zygote

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Currently, rescue in vitro maturation (IVM) is not a routine method in assisted reproductive treatment (ART) programmes but is a promising procedure for ART to improve IVM. The aim of this study was to compare embryo morphokinetics of germinal vesicles (GV) with metaphase II (MII) oocytes from controlled ovarian hyperstimulation (COH) cycles by timelapse photography monitoring (TLM). Morphokinetics of the same number of embryos derived from the in vivo (group I) and rescue of in vitro matured oocytes (group II) from 310 patients were analyzed and compared retrospectively. The time to form second PB extrusion (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPNf) and time of two to eight discrete cells (t2-t8) were assessed. Abnormal cleavage patterns such as uneven blastomeres at the two-cell stage, cell fusion (Fu), trichotomous mitoses (TM), and the rates of embryo arrest were assessed. These data showed that tPB2, tPNa, tPNf, t2, t3 and t4 stages took place later in group II compared with group I (P < 0.001, P = 0.017, P < 0.001, P < 0.001, P < 0.001, P < 0.001, respectively). The rates of uneven blastomeres, Fu, TM, and embryo arrest were increased significantly in group II compared with group I (P = 0.001, P < 0.001, P = 0.003, P < 0.001, respectively). Based on the exact annotation of timing parameters and cleavage patterns, the present data agreed with the concept that rescue IVM of oocytes negatively influences embryo morphokinetics. Therefore, cautious use of embryos derived from rescue IVM of GV oocytes should be made. Zygote cambridge.org/zyg Research Article Cite this article: Faramarzi et al. (2018) Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development? Zygote 26: 430-434.

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Section: Discussionmentioning

confidence: 99%

“…Following controlled ovarian hyperstimulation (COH), approximately 20% of oocytes are immature (Ashourzadeh et al , 2015). These oocytes are usually discarded in the majority of the IVF programmes (Lee et al , 2016).…”

Section: Introductionmentioning

confidence: 99%

Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development?

Faramarzi

Khalili

Ashourzadeh

et al. 2018

Zygote

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“…Rescue IVM of these immature oocytes can increase the chances of embryo access, especially in poor responders [1,2]. In addition, the IVM technique plays a highly important role in the elimination of ovarian hyperstimulation syndrome, which is a possible side effect of gonadotropin stimulation in patients with polycystic ovary syndrome (PCOS) [3,4]. Oocytes have the ability to influence gene expression in cumulus cells (CCs) and mural cells, even in the presence of follicle-stimulating hormone (FSH), which indicates that the intracellular behavior of CCs is affected by their close relationship with the oocyte [5].…”

Section: Introductionmentioning

confidence: 99%

Growth differentiation factor 9 and cumulus cell supplementation in in vitro maturation culture media enhances the viability of human blastocysts

Chatroudi

Khalili

Ashourzadeh

et al. 2019

Clin Exp Reprod Med

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Objective: In vitro maturation (IVM) of immature oocytes can be useful for some infertile patients. In IVM programs, the rates of embryo formation and pregnancy are low. Therefore, it is essential to recognize the main factors involved in regulating oocyte maturation in vitro. The purpose of this study was to investigate the effects of growth differentiation factor 9 (GDF9) and cumulus cell (CC) supplementation in IVM medium on the rates of embryo formation and viability of human blastocysts. Methods: A total of 80 germinal vesicle oocytes from stimulated cycles underwent an IVM program. The oocytes were divided into four groups, where group I consisted of IVM media only and served as the control, group II consisted of IVM+CCs, group III consisted of IVM+GDF9 (200 ng/mL), and group IV consisted of IVM+CCs+GDF9 (200 ng/mL). Intracytoplasmic sperm injection was performed on the IVM oocytes, and the cleavage embryos that were generated were vitrified. Following thawing, the embryos were cultured for 3 additional days, and the viability rates of the developed blastocysts were determined. Results: The maturation rate of the oocytes did not differ significantly across the four groups. The fertilization rate in group II was significantly higher than that in the control group (76.5% vs. 46.2%). Embryo formation was significantly more frequent in all experimental groups than in the control group, while blastocyst formation did not show significant differences in the three experimental groups compared to the control. The mean viability rates in groups II, III, and IV were 58.16%, 55.91%, and 55.95%, respectively, versus 37.78% in the control group (p< 0.05). Conclusion: Supplementation of IVM culture media with GDF9 and CCs enhanced the fertilization, embryo formation, and viability rates of blastocysts generated from vitrified cleavage embryos. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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“…However, the selection of cattle oocytes by the presence of a PLM signal does not seem to offer improvement in IVP outcomes yet. In addition, PLM has been used for the evaluation of the ZPB, which in humans has been associated to oocyte quality [116][117][118], although it is still under debate [119,120]. The few studies in cattle have shown that a lower ZPB is related to high quality oocytes and improved blastocyst development [121,122], whereas two studies in horses have reported conflicting results, indicating beneficial effects of both low ZPB [123] and high ZPB [124].…”

Section: Evaluation Of the Meiotic Spindle And Zona Pellucida Birefrimentioning

confidence: 99%

Oocyte Selection for <em>in vitro</em> embryo Production in Bovine Species: Non-Invasive Approaches for the New Challenges of Oocyte Competence

Águila¹,

Treulén²,

Therrien³

et al. 2020

Preprint

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The efficiency of producing embryos using in vitro technologies in livestock species rarely exceeds the 30 to 40% threshold, indicating that the proportion of oocytes that fail to develop after in vitro fertilization and culture is considerably large. Considering that the intrinsic quality of the oocyte is one of the main factors affecting blastocyst yield, the precise identification of non-invasive cellular or molecular markers that predict oocyte competence is of major interest to research and practical applications. The aim of this review was to explore the current literature on different non-invasive markers associated with oocyte quality in the bovine model. Apart from some controversial findings, the presence of cycle-related structures in ovaries, a follicle size between 6 and 10 mm, large number of surrounding cumulus cells, slightly expanded investment without dark areas, large oocyte diameter (>120 microns), dark cytoplasm, and the presence of a round and smooth first polar body have been associated to better competence. In addition, the combination of oocyte and zygote selection by BCB test and spindle imaging have the potential to further optimize the identification of oocytes with better developmental competence for in vitro-derived technologies in livestock species.

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Noninvasive assays of in vitro matured human oocytes showed insignificant correlation with fertilization and embryo development

Abstract: There were insignificant relationships between the MS detection and ZP birefringence score with the rates of fertilization and embryo development in IVM oocytes.

Cited by 12 publications

References 23 publications

Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development?

Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development?

Growth differentiation factor 9 and cumulus cell supplementation in in vitro maturation culture media enhances the viability of human blastocysts

Oocyte Selection for <em>in vitro</em> embryo Production in Bovine Species: Non-Invasive Approaches for the New Challenges of Oocyte Competence

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