Noninvasive blood flow imaging for real‐time feedback during laser therapy of port wine stain birthmarks

Huang, Yu-Chih; Ringold, Tyson L.; Nelson, J. Stuart; Choi, Bernard

doi:10.1002/lsm.20619

Cited by 67 publications

(52 citation statements)

References 21 publications

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“…15 Laser Doppler and laser speckle contrast imaging can provide wide-field relative measurements of microvessel perfusion. 18,67,68 OCT can provide absolute blood velocity and volume flow measurements, 23,24,69 as well as microvessel hematocrit measurements 30 and relative saturation. 29 Photoacoustic tomography can be used to image microvasculature and measure HbSat to depths of up to 3 mm in tissue.…”

Section: Discussionmentioning

confidence: 99%

Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses

et al. 2010

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Abstract. Abnormal microvascular physiology and function is common in many diseases. Numerous pathologies include hypervascularity, aberrant angiogenesis, or abnormal vascular remodeling among the characteristic features of the disease, and quantitative imaging and measurement of microvessel function can be important to increase understanding of these diseases. Several optical techniques are useful for direct imaging of microvascular function. Spectral imaging is one such technique that can be used to assess microvascular oxygen transport function with high spatial and temporal resolution in microvessel networks through measurements of hemoglobin saturation. We highlight novel observation made with our intravital microscopy spectral imaging system employed with mouse dorsal skin-fold window chambers for imaging hemoglobin saturation in microvessel networks. Specifically, we image acute oxygenation fluctuations in a tumor microvessel network, the development of arteriovenous malformations in a mouse model of hereditary hemorrhagic telangiectasia, and the formation of spontaneous and induced microvascular thromboses and occlusions.

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Section: Discussionmentioning

confidence: 99%

Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses

et al. 2010

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“…Although laser therapy is now the standard treatment for PWS, some areas of residual perfusion can exist following treatment. LSCI has the potential to provide real-time feedback during PWS treatment by indicating the spatial distribution of perfusion in the skin before and after laser therapy, as illustrated 41 in Fig. 4.…”

Section: Skin Perfusionmentioning

confidence: 99%

Laser speckle contrast imaging in biomedical optics

Boas¹,

2010

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Abstract. First introduced in the 1980s, laser speckle contrast imaging is a powerful tool for full-field imaging of blood flow. Recently laser speckle contrast imaging has gained increased attention, in part due to its rapid adoption for blood flow studies in the brain. We review the underlying physics of speckle contrast imaging and discuss recent developments to improve the quantitative accuracy of blood flow measures. We also review applications of laser speckle contrast imaging in neuroscience, dermatology and ophthalmology.

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“…Beyond bench-side physiological research, there are direct implications for prognostic, diagnostic, and intraoperative imaging applications, as speckle imaging of microcirculatory flows is increasingly becoming applied for gauging local and systemic tissue health [8,9]. Consequently, laser speckle flowmetry studies are expanding in dermatological [10][11][12][13][14], ophthalmological [15][16][17], and neurosurgical settings [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Optimization of camera exposure durations for multi-exposure speckle imaging of the microcirculation

Kazmi

Balial

Dunn

2014

Biomed. Opt. Express

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Improved Laser Speckle Contrast Imaging (LSCI) blood flow analyses that incorporate inverse models of the underlying laser-tissue interaction have been used to develop more quantitative implementations of speckle flowmetry such as Multi-Exposure Speckle Imaging (MESI). In this paper, we determine the optimal camera exposure durations required for obtaining flow information with comparable accuracy with the prevailing MESI implementation utilized in recent in vivo rodent studies. A looping leave-one-out (LOO) algorithm was used to identify exposure subsets which were analyzed for accuracy against flows obtained from analysis with the original full exposure set over 9 animals comprising n = 314 regional flow measurements. From the 15 original exposures, 6 exposures were found using the LOO process to provide comparable accuracy, defined as being no more than 10% deviant, with the original flow measurements. The optimal subset of exposures provides a basis set of camera durations for speckle flowmetry studies of the microcirculation and confers a twofold faster acquisition rate and a 28% reduction in processing time without sacrificing accuracy. Additionally, the optimization process can be used to identify further reductions in the exposure subsets for tailoring imaging over less expansive flow distributions to enable even faster imaging.

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Noninvasive blood flow imaging for real‐time feedback during laser therapy of port wine stain birthmarks

Cited by 67 publications

References 21 publications

Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses

Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses

Laser speckle contrast imaging in biomedical optics

Optimization of camera exposure durations for multi-exposure speckle imaging of the microcirculation

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