Noninvasive imaging of human skin hemodynamics using a digital red-green-blue camera

Nishidate, Izumi; Тanaka, Noriyuki; Kawase, Tatsuya; Maeda, Takao; Yuasa, Tomonori; Aizu, Yoshihisa; Yuasa, Tetsuya; Niizeki, Kyuichi

doi:10.1117/1.3613929

Cited by 70 publications

(61 citation statements)

References 36 publications

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“…There is an initial overshoot of the oxy and deoxyhemoglobin values followed by a return to baseline. These results are similar to previous in vivo measurements of human tissue hemodynamics during arterial occlusion using SFDI (17) and other techniques (38).…”

Section: Resultssupporting

confidence: 82%

In Vivo Multiphoton NADH Fluorescence Reveals Depth-Dependent Keratinocyte Metabolism in Human Skin

Balu

Mazhar

Hayakawa

et al. 2013

Optics in the Life Sciences

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We employ a clinical multiphoton microscope to monitor in vivo and noninvasively the changes in reduced nicotinamide adenine dinucleotide (NADH) fluorescence of human epidermal cells during arterial occlusion. We correlate these results with measurements of tissue oxy-and deoxyhemoglobin concentration during oxygen deprivation using spatial frequency domain imaging. During arterial occlusion, a decrease in oxyhemoglobin corresponds to an increase in NADH fluorescence in the basal epidermal cells, implying a reduction in basal cell oxidative phosphorylation. The ischemia-induced oxygen deprivation is associated with a strong increase in NADH fluorescence of keratinocytes in layers close to the stratum basale, whereas keratinocytes from epidermal layers closer to the skin surface are not affected. Spatial frequency domain imaging optical property measurements, combined with a multilayer Monte Carlo-based radiative transport model of multiphoton microscopy signal collection in skin, establish that localized tissue optical property changes during occlusion do not impact the observed NADH signal increase. This outcome supports the hypothesis that the vascular contribution to the basal layer oxygen supply is significant and these cells engage in oxidative metabolism. Keratinocytes in the more superficial stratum granulosum are either supplied by atmospheric oxygen or are functionally anaerobic. Based on combined hemodynamic and two-photon excited fluorescence data, the oxygen consumption rate in the stratum basale is estimated to be~0.035 mmoles/10 6 cells/h.

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Section: Resultssupporting

confidence: 82%

In Vivo Multiphoton NADH Fluorescence Reveals Depth-Dependent Keratinocyte Metabolism in Human Skin

Balu

Mazhar

Hayakawa

et al. 2013

Optics in the Life Sciences

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“…The optimization problem for the chromophore estimation was adapted to actual skin reflectance spectrum. The same time courses of changes in chromopohore concentrations with the result reported in literature [10,21].…”

Section: Discussionsupporting

confidence: 76%

“…Figure 9(b) shows the time courses of C m , C oh , C dh , C th , and StO 2 during cuff occlusion at 250 mm Hg. The average values of C m and C th were 6.24 ± 0.06% and 0.211 ± 0.008%, respectively, in pre-occlusion (normal), which are close to typical values for Japanese subjects reported in the literatures [10,21,22]. The average value of 44.3 ± 3.4% for StO 2 in pre-occlusion agrees with the mean blood oxygen saturation of normal human subjects (range 30.2-52.4%) reported in the literatures [23].…”

Section: Estimating Chromophore Concentrations From the Cmcs Spectrumsupporting

confidence: 65%

Rapid calculation of diffuse reflectance from a multilayered model by combination of the white Monte Carlo and adding-doubling methods

Yoshida

Nishidate

2014

Biomed. Opt. Express

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Abstract:To rapidly derive a result for diffuse reflectance from a multilayered model that is equivalent to that of a Monte-Carlo simulation (MCS), we propose a combination of a layered white MCS and the addingdoubling method. For slabs with various scattering coefficients assuming a certain anisotropy factor and without absorption, we calculate the transition matrices for light flow with respect to the incident and exit angles. From this series of precalculated transition matrices, we can calculate the transition matrices for the multilayered model with the specific anisotropy factor. The relative errors of the results of this method compared to a conventional MCS were less than 1%. We successfully used this method to estimate the chromophore concentration from the reflectance spectrum of a numerical model of skin and in vivo human skin tissue.

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“…The method for estimating blood concentration from RGB values was proposed by Nishidate et al [18,19,20]; this process is shown in Figure 2 and can be divided into three steps, as follows: Step 1 We calculated the color transformation matrix N 1 that can transform RGB values into Commission Internationale de l’Éclairage XYZ (CIEXYZ) tristimulus values. The matrix N 1 was determined in advance using a standard color chart.…”

Section: Methodsmentioning

confidence: 99%

Visualization of Venous Compliance of Superficial Veins Using Non-Contact Plethysmography Based on Digital Red-Green-Blue Images

Nakano

Aoki

Satoh

et al. 2016

Sensors

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We propose the visualization of venous compliance (VC) using a digital red-green-blue (RGB) camera. The new imaging method, which transforms RGB values into VC, combines VC evaluation with blood concentration estimation from the RGB values of each pixel. We evaluate a non-contact plethysmography (NCPG) system for VC based on comparisons with conventional strain gauge plethysmography (SPG). We conduct in vivo measurements using both systems and investigate their differences by evaluating the VC. The results show that the two methods measure different blood vessels and that errors caused by interstitial fluid accumulation are negligible for the NCPG system, whereas SPG is influenced by such errors. Additionally, we investigate the relationship between VC and physical activity using NCPG.

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Noninvasive imaging of human skin hemodynamics using a digital red-green-blue camera

Cited by 70 publications

References 36 publications

In Vivo Multiphoton NADH Fluorescence Reveals Depth-Dependent Keratinocyte Metabolism in Human Skin

In Vivo Multiphoton NADH Fluorescence Reveals Depth-Dependent Keratinocyte Metabolism in Human Skin

Rapid calculation of diffuse reflectance from a multilayered model by combination of the white Monte Carlo and adding-doubling methods

Visualization of Venous Compliance of Superficial Veins Using Non-Contact Plethysmography Based on Digital Red-Green-Blue Images

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