Noninvasive photoacoustic imaging of the developing vasculature during early tumor growth

Lao, Yeqi; Xing, Da; Yang, Sihua; Xiang, Liangzhong

doi:10.1088/0031-9155/53/15/013

Cited by 143 publications

(106 citation statements)

References 23 publications

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“…18,19 Photoacoustic imaging can be used for in vivo imaging at reasonable depths, 20 as well as for longitudinal studies. 21,22 Specifically, the long-term goal of our study is to assess optically how MSCs delivered in vivo via a PEGylated fibrin gel participate in the process of n eovascularization. In this study, we assessed the efficiency of MSC labeling with gold nanotracers and the effects of nanoparticle loading on cell function.…”

Section: Introductionmentioning

confidence: 99%

Function of mesenchymal stem cells following loading of gold nanotracers

Ricles¹,

Nam²,

Sokolov

et al. 2011

IJN

101

117

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Background: Stem cells can differentiate into multiple cell types, and therefore can be used for cellular therapies, including tissue repair. However, the participation of stem cells in tissue repair and neovascularization is not well understood. Therefore, implementing a noninvasive, long-term imaging technique to track stem cells in vivo is needed to obtain a better understanding of the wound healing response. Generally, we are interested in developing an imaging approach to track mesenchymal stem cells (MSCs) in vivo after delivery via a polyethylene glycol modified fibrin matrix (PEGylated fibrin matrix) using MSCs loaded with gold nanoparticles as nanotracers. The objective of the current study was to assess the effects of loading MSCs with gold nanoparticles on cellular function. Methods: In this study, we utilized various gold nanoparticle formulations by varying size and surface coatings and assessed the efficiency of cell labeling using darkfield microscopy. We hypothesized that loading cells with gold nanotracers would not significantly alter cell function due to the inert and biocompatible characteristics of gold. The effect of nanoparticle loading on cell viability and cytotoxicity was analyzed using a LIVE/DEAD stain and an MTT assay. The ability of MSCs to differentiate into adipocytes and osteocytes after nanoparticle loading was also examined. In addition, nanoparticle loading and retention over time was assessed using inductively coupled plasma mass spectrometry (ICP-MS). Conclusion: Our results demonstrate that loading MSCs with gold nanotracers does not alter cell function and, based on the ICP-MS results, long-term imaging and tracking of MSCs is feasible. These findings strengthen the possibility of imaging MSCs in vivo, such as with optical or photoacoustic imaging, to understand better the participation and role of MSCs in neovascularization.

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Section: Introductionmentioning

confidence: 99%

Function of mesenchymal stem cells following loading of gold nanotracers

Ricles¹,

Nam²,

Sokolov

et al. 2011

IJN

101

117

View full text Add to dashboard Cite

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“…79 Similarly, hemoglobin is a suitable indicator to demonstrate the degree of neovascularization in tumors, and the morphology and density increase of vasculature could be clearly displayed by means of PAI. 80 In addition, targeted exogenous contrast agents can be applied to further enhance the contrast so that tiny microvasculature in tumors can be visualized.…”

Section: 6477 Cancer Detectionmentioning

confidence: 99%

Biomedical photoacoustics: fundamentals, instrumentation and perspectives on nanomedicine

Zou¹,

Wu²,

Dong³

et al. 2016

IJN

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“…Compared to light scattering of visible or NIR light, ultrasound is scattered several orders of magnitude less in tissue, resulting in depth penetration of several cm, while maintaining a resolution of $100 mm or less. Because hemoglobin presents strong light absorption in the visible, optoacoustic imaging is well suited for resolving vascular structures [28]. In addition, multispectral illumination at several wavelengths combined with spectral unmixing can reveal specific chromophores and fluorescent proteins, distinguishing between oxy-and deoxyhemoglobin, a technique termed multispectral optoacoustic tomography (MSOT) [29,30].…”

Section: Optoacoustic Imagingmentioning

confidence: 99%