Noninvasive Sweat-Lactate Biosensor Emplsoying a Hydrogel-Based Touch Pad

Nagamine, Kuniaki; Mano, Taisei; Nomura, Ayako; Ichimura, Yusuke; Izawa, Ryota; Furusawa, Hiroyuki; Matsui, Hiroyuki; Kumaki, Daisuke; Tokito, Shizuo

doi:10.1038/s41598-019-46611-z

Cited by 106 publications

(81 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…22−24 Both interface types, however, are commonly used only as reservoirs for subsequent analysis in a decoupled manner with bulky laboratory instruments (e.g., mass spectrometers). Recently, a proof-of-concept of lactate measurement with a sensor-coupled hydrogel was demonstrated for fingertip sweat sampling; 25 however, the key hydrogel sensor performance metrics (including the sensor's limit of detection (LoD), detection range, and selectivity) has not been fully characterized, and its deployment in diverse application spaces has not been demonstrated.…”

mentioning

confidence: 99%

Natural Perspiration Sampling and in Situ Electrochemical Analysis with Hydrogel Micropatches for User-Identifiable and Wireless Chemo/Biosensing

et al. 2019

View full text Add to dashboard Cite

Recent advances in microelectronics, microfluidics, and electrochemical sensing platforms have enabled the development of an emerging class of fully integrated personal health monitoring devices that exploit sweat to noninvasively access biomarker information. Despite such advances, effective sweat sampling remains a significant challenge for reliable biomarker analysis, with many existing methods requiring active stimulation (e.g., iontophoresis, exercise, heat). Natural perspiration offers a suitable alternative as sweat can be collected with minimal effort on the part of the user. To leverage this phenomenon, we devised a thin hydrogel micropatch (THMP), which simultaneously serves as an interface for sweat sampling and a medium for electrochemical sensing. To characterize the performance of the THMP, caffeine and lactate were selected as two representative target molecules. We demonstrated the suitability of the sampling method to track metabolic patterns, as well as to render sample-to-answer biomarker data for personal monitoring (through coupling with an electrochemical sensing system). To inform its potential application, this biomarker sampling and sensing system is incorporated within a distributed terminal-based sensing network, which uniquely capitalizes on the fingertip as a site for simultaneous biomarker data sampling and user identification.

show abstract

mentioning

confidence: 99%

Natural Perspiration Sampling and in Situ Electrochemical Analysis with Hydrogel Micropatches for User-Identifiable and Wireless Chemo/Biosensing

et al. 2019

View full text Add to dashboard Cite

show abstract

“…These sensors have shown that they could detect analytes in the concentration range found in human sweat components. Our research group also has focused on developing extended-gate organic transistor-based biosensors for sweat analysis utilizing different types of bioreceptors: antibody, [53][54][55][56][57] enzyme, [58][59][60][61][62][63][64][65][66] and ionophore, 67,68 because, as described below, the organic transistor can be mass-fabricated at low cost using advanced printed technology. The following typical fabrication process was used for our extended gate-type, organic, transistor-based biosensor.…”

Section: Transistor-based Biosensors For Highly Sensitive Sweat Analysismentioning

confidence: 99%

“…Based on these factors, we invented a new sweat extraction and detection device. 66 The sensor was composed of an enzymebased L-lactate sensor and Ag/AgCl reference electrodes, prepared in the same way as described in Fig. 3(a), and were fully covered with an agarose hydrogel including a sweatextraction solution of PBS ( Fig.…”

Section: Sweat Biomarkers and Their Collection/ Detection Techniquesmentioning

confidence: 99%

Printed Organic Transistor-based Biosensors for Non-invasive Sweat Analysis

et al. 2020

Self Cite

View full text Add to dashboard Cite

The concept of the Internet of Things (IoT), which connects objects to a network via various types of sensors to solve global societal needs, is now spreading to various application fields to achieve an emerging "smart lifestyle". Healthcare is one of the most attractive applications of IoT, because it provides various healthcare services for individuals, including daily health monitoring, fitness support, and elderly care. To realize these applications, there is a need to develop simple portable sensor devices that can be used anywhere, including non-medical

show abstract

“…The redox reaction can be also used in potentiometric sensors, [17,18] in which the electron transfer generates open-circuit potential between the reference and enzyme-functionalized electrode. Others include colorimetric sensing that utilizes electrochemical colorimetric indicators, [19] and transistor-based sensing that includes enzyme-functionalized electrodes in transistor configuration.…”

Section: Doi: 101002/mabi202000144mentioning

confidence: 99%

A Flexible 3D Organic Preamplifier for a Lactate Sensor

Baek

Kwon

Mano

et al. 2020

Macromolecular Bioscience

Self Cite

View full text Add to dashboard Cite

Organic transistors are promising platforms for wearable biosensors. However, the strategies to improve signal amplification have yet to be determined, particularly regarding biosensors that generate very weak signals. In this study, an organic voltage amplifier is presented for a lactate sensor on flexible plastic foil. The preamplifier is based on a 3D complementary inverter, which is achieved by vertically stacking complementary transistors with a shared gate between them. The shared gate is extended and functionalized with a lactate oxidase enzyme to detect lactate. The sensing device successfully detects the lactate concentration in the human sweat range (20–60 mm) with high sensitivity (6.82 mV mm−1) due to high gain of its amplification. The 3D integration process is cost‐effective as it is solution‐processable and doubles the number of transistors per unit area. The device presented in this study would pave the way for the development of high‐gain noninvasive sweat lactate sensors that can be wearable.

show abstract

Noninvasive Sweat-Lactate Biosensor Emplsoying a Hydrogel-Based Touch Pad

Cited by 106 publications

References 30 publications

Natural Perspiration Sampling and in Situ Electrochemical Analysis with Hydrogel Micropatches for User-Identifiable and Wireless Chemo/Biosensing

Natural Perspiration Sampling and in Situ Electrochemical Analysis with Hydrogel Micropatches for User-Identifiable and Wireless Chemo/Biosensing

Printed Organic Transistor-based Biosensors for Non-invasive Sweat Analysis

A Flexible 3D Organic Preamplifier for a Lactate Sensor

Contact Info

Product

Resources

About