Nonlinear fiber amplifier with tunable transform limited pulse duration from a few 100 to sub-100-fs at watt-level powers

Domingue, Scott R.; Bartels, Randy A.

doi:10.1364/ol.39.000359

Cited by 14 publications

(8 citation statements)

References 14 publications

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“…An all-normal dispersion fiber oscillator [36] seeds a nonlinear fiber amplifier [35], outputting 1.3 W (21 nJ / pulses at a repetition rate of 63 MHz) and pulse durations of 140 fs after com- pression. The net transmission efficiency through the fiber cocktail, or segmented fiber, is 60%.…”

Section: Source Characterizationmentioning

confidence: 99%

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Transient absorption imaging of hemes with 2-color, independently tunable visible-wavelength ultrafast source

Domingue¹,

Bartels²,

Chicco³

et al. 2017

Biomed. Opt. Express

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Pump probe microscopy is a time-resolved multiphoton imaging technique capable of generating contrast between non-fluorescent pigments based on differences in excited-state lifetimes. Here we describe a fiber-based ultrafast system designed for imaging heme proteins with an independently-tunable pulse pair in the visible-wavelength regime. Starting with a 1060 nm fiber amplifier (1.3 W at 63 MHz, 140 fs pulses), visible pulses were produced in the vicinity of 488 nm and 532 nm by doubling the output of a short photonic crystal fiber with a pair of periodically-poled lithium niobate crystals, providing 5-20 mW power in each beam. This was sufficient for acquiring transient absorption images from unstained cryosectioned tissue.

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Section: Source Characterizationmentioning

confidence: 99%

“…Here we expand this approach to provide independently-tunable pump and probe pulses, seeded by an inexpensive homebuilt fiber oscillator and amplifier [35]. Our system currently provides 5-20 mW power in each beam (75-300 pJ / pulse), with tuning ranges of 485-495 nm, and 525-540 nm determined by phase matching conditions.…”

Section: Introductionmentioning

confidence: 99%

Transient absorption imaging of hemes with 2-color, independently tunable visible-wavelength ultrafast source

Domingue¹,

Bartels²,

Chicco³

et al. 2017

Biomed. Opt. Express

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“…The optical setup is described in detail in refs and . Briefly, a high-repetition-rate (63 MHz) train of λ pu = 530 nm and λ pr = 490 nm pulses is generated from an amplified 1060 nm fiber laser by nonlinear broadening followed by frequency doubling .…”

Section: Experimental Methodsmentioning

confidence: 99%

High-Repetition-Rate Transient Absorption Spectroscopy of Respiratory Supercomplexes

et al. 2022

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Hemeproteins are frequent subjects for ultrafast transient absorption spectroscopy (TAS) because of biological importance, strong UV–vis absorption, high photostability, and interesting transient dynamics that depend on redox, conformation, and ligand binding. TAS on hemeproteins is usually performed on isolated, purified proteins, though their response is likely to be different in their native molecular environment, which involves the formation of protein complexes and supercomplexes. Recently, we reported a transient absorption microscopy (TAM) experiment which elicited a transient response from hemeproteins in intact biological tissue using a visible-wavelength pump (530 nm) and probe (490 nm). Here, we find that adaptive noise canceling plus resonant galvanometer scanning enables a high-repetition-rate fiber laser source to make redox-sensitive measurements of cytochrome c (Cyt-c). We investigate the origins of the visible-wavelength response of biological tissue through TAS of intact mitochondrial respiratory supercomplexes, separated via gel electrophoresis. We find that each of these high-molecular-weight gel bands yields a TAS response characteristic of cytochrome hemes, implying that the TAS response of intact cells and tissue originates from not just Cyt-c but a mixture of respiratory cytochromes. We also find differences in excited-state lifetime between wild-type (WT) and a tafazzin-deficient (TAZ) mouse model of mitochondrial disease.

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“…The microscope system was illuminated by pulses from a nonlinear fiber amplifier centered at 1,065 nm (36), seeded by an all-normal dispersion fiber oscillator using Yb-doped gain fiber (37). Temporal compression in a folded Martinez compressor resulted in near transform-limited durations of ∼150 fs at an average power ranging from 900 mW to 1.3 W and a repetition rate of 52.5 MHz.…”

Section: Methodsmentioning

confidence: 99%

Superresolved multiphoton microscopy with spatial frequency-modulated imaging

Field

Wernsing

Domingue

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Superresolved far-field microscopy has emerged as a powerful tool for investigating the structure of objects with resolution well below the diffraction limit of light. Nearly all superresolution imaging techniques reported to date rely on real energy states of fluorescent molecules to circumvent the diffraction limit, preventing superresolved imaging with contrast mechanisms that occur via virtual energy states, including harmonic generation (HG). We report a superresolution technique based on spatial frequencymodulated imaging (SPIFI) that permits superresolved nonlinear microscopy with any contrast mechanism and with single-pixel detection. We show multimodal superresolved images with twophoton excited fluorescence (TPEF) and second-harmonic generation (SHG) from biological and inorganic media. Multiphoton SPIFI (MP-SPIFI) provides spatial resolution up to 2η below the diffraction limit, where η is the highest power of the nonlinear intensity response. MP-SPIFI can be used to provide enhanced resolution in optically thin media and may provide a solution for superresolved imaging deep in scattering media.superresolution | harmonic generation | multiphoton microscopy

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Nonlinear fiber amplifier with tunable transform limited pulse duration from a few 100 to sub-100-fs at watt-level powers

Cited by 14 publications

References 14 publications

Transient absorption imaging of hemes with 2-color, independently tunable visible-wavelength ultrafast source

Transient absorption imaging of hemes with 2-color, independently tunable visible-wavelength ultrafast source

High-Repetition-Rate Transient Absorption Spectroscopy of Respiratory Supercomplexes

Superresolved multiphoton microscopy with spatial frequency-modulated imaging

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