In this study, tensile splitting tests and corresponding numerical simulations are performed on high strength concrete, low strength concrete, the interface between the two types of concrete, and the interface reinforced by steel fiber, respectively. The tensile splitting strength, crack initiation load, and damage characteristics are analyzed based on experiment data and fracture surface of samples. It can be concluded that tensile splitting strength and crack initiation load have the descending order of ‘HT’ (high strength concrete sample) > ‘LT’ (low strength concrete sample) > ‘FT’ (interface reinforced by steel fiber) > ‘OT’ (interface). The tensile splitting strength is related not only to the roughness of the fracture surface, but also to the percentage of fractured aggregates. The steel fiber can increase initiation cracking load, peak load and residual strength of the interface. The existing of interface composited by two types of material can cause asymmetric distribution of the stress state, even if geometry and the load are symmetrical for samples.