Nonmelting-flesh Trait in Peaches Is Not Related to Low Ethylene Production Rates

Brovelli, Ernesto A.; Brecht, Jeffrey K.; Sherman, W. B.; Sims, Charles A.

doi:10.21273/hortsci.34.2.313

Cited by 20 publications

(20 citation statements)

References 14 publications

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“…The ripening of climacteric fruits, as peach and nectarine, involves a complex, genetically programmed process, which is accompanied by a transient burst of ethylene production 4. However, it has been postulated that the ethylene climacteric peak cannot be connected with the degree of flesh softening and MF or NMF characteristic of fruits,23 agreeing with the results of the present work. The same conclusion has been reached by other researchers (Tonutti P, personal communication).…”

Section: Resultssupporting

confidence: 91%

Diverse metabolism of cell wall components of melting and non‐melting peach genotypes during ripening after harvest or cold storage

et al. 2005

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The mode of change in a range of physiological, physicochemical and biochemical parameters during fruit ripening between distinct peach genotypes (Prunus persica L. Batsch) after cold storage for up to 4 weeks was determined. The nectarine cultivar 'Caldesi 2000' was selected as a genotype with melting flesh (MF) characteristic (fruits characterized by extensive flesh softening during ripening) and the cultivar 'Andross' as a genotype with non-melting flesh (NMF) characteristic (fruits characterized by limited flesh softening during ripening). Flesh firmness, ethylene production, physicochemical and biochemical properties of the cell wall were determined and significant differences between the fruits of the two genotypes were recorded. Fruits of the NMF genotype were characterized by higher tissue retention and ethylene production during their ripening, higher content of uronic acids, as well as higher capacity for calcium binding in the water-insoluble pectin fraction compared with fruits of the MF genotype. Additionally, the ripening of MF-type fruits was characterized by higher losses of neutral sugars, especially those of arabinose and galactose than the NMF-type fruits and these losses were more intense after extended cold storage periods. In fruits of the NMF genotype the decreased activity of pectin methyl esterase (PME) combined with higher levels of calcium in the water-insoluble pectin fraction possibly provided less substrate for polygalacturonase (exo-, endo-PG) activity and less solubility of cell wall pectin compared with fruits of the MF genotype. Overall, the data indicate the existence of a wide range of diverse metabolic pathways during fruit ripening of fresh fruits with MF and NMF characteristics.

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Section: Resultssupporting

confidence: 91%

Diverse metabolism of cell wall components of melting and non‐melting peach genotypes during ripening after harvest or cold storage

et al. 2005

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“…Fruit ethylene emission appeared earlier and was greater in Oro A than in Springcrest and in Sanguinella (Fig. ), consistent with reports showing that NMF peaches generally evolve higher amounts of ethylene than MF ones (Brovelli et al , Morgutti et al , , Kao et al ).…”

Section: Discussionsupporting

confidence: 88%

Cinnamyl alcohol dehydrogenases in the mesocarp of ripening fruit of Prunus persica genotypes with different flesh characteristics: changes in activity and protein and transcript levels

Gabotti

Negrini

Morgutti

et al. 2015

Physiologia Plantarum

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Development of fruit flesh texture quality traits may involve the metabolism of phenolic compounds. This study presents molecular and biochemical results on the possible role played by cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) during ripening [S3, S4 I (pre-climacteric) and S4 III (climacteric) stages] of peach [Prunus persica (L.) Batsch] fruit with different flesh firmness [non-melting flesh (NMF) 'Oro A'/melting flesh (MF) 'Springcrest' and 'Sanguinella'] and color (blood-flesh Sanguinella). A total of 24 putative full-length PRUPE_CAD genes were identified (in silico analysis) in the peach genome. The most abundant CAD isoforms, encoded by genes located on scaffolds 8 and 6, were probed by specifically developed anti-PRUPE_CAD sc8 and by anti-FaCAD (PRUPE_CAD sc6) polyclonal antibodies, respectively. PRUPE_CAD sc8 proteins (SDS-PAGE and native-PAGE/western blot) appeared responsible for the CAD activity (in vitro/in-gel assays) that increased with ripening (parallel to PRUPE_ACO1 transcripts accumulation and ethylene evolution) only in the mesocarp of Oro A and blood-flesh Sanguinella. Accumulation of PRUPE_CAD sc8 transcripts (semi-quantitative RT-PCR) occurred in all three cultivars, but in Oro A and Springcrest it was not always accompanied by that of the related proteins, suggesting possible post-transcriptional regulation. Flesh firmness, as well as levels of lignin, total phenolics and, where present (Sanguinella), anthocyanins, declined with ripening, suggesting that, at least in the studied peach cultivars, CAD activity is related to neither lignification nor differences in flesh firmness (NMF/MF). Further studies are necessary to clarify whether the high levels of CAD activity/expression in Sanguinella play a role in determining the characteristics of this blood-flesh fruit.

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“…Greater FBL in CNMF fruit may be an indirect pleiotropic effect of the lack of endoPG activity. Elevated ethylene production seems to be a common feature of CNMF cultivars (Brovelli et al 1999;Manganaris et al 2006) and may be part of a biochemical cascade that influences the spread of pigment in CNMF fruit, but not FMF fruit. Alternatively, another polymorphic gene closely linked to the F-M locus may be part of the control mechanism for FBL; if so, it must lie very close to F-M locus since the observed QTL peak coincides with this locus (Ogundiwin et al 2007).…”

Section: Inheritance Of CI Symptomsmentioning

confidence: 99%

“…Mealiness (M) in CNMF fruit used for canning is not a problem, as fruit are quickly processed after harvest and do not require long cold storage, and FBL in fresh market fruit is acceptable to consumers. Fresh market CNMF fruit is one way to avoid M (Brovelli et al 1998), but consumers tend to prefer the melting texture (Brovelli et al 1999). However, there is hope for creating new cultivars with genetic resistance to M in FMF fruit or FBL in CNMF fruit.…”

Section: Relationships Among CI Symptomsmentioning

confidence: 99%

Influence of year and genetic factors on chilling injury susceptibility in peach (Prunus persica (L.) Batsch)

et al. 2011

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Chilling injury (CI) is a major physiological problem limiting consumption and export of peach and nectarine (Prunus persica (L.) Batsch). To clarify the genetic basis for chilling injury, inheritance of the major CI symptoms mealiness, flesh browning, flesh bleeding, and flesh leatheriness were examined over three years in two related peach progenies. In addition, genetic relationships among traits and the year-to-year variation in trait performance in these progenies were tracked. Both populations also segregated for Freestone-Melting flesh (F-M) and yellow flesh. There were significant differences in CI symptoms among years. The major gene endoPG, which controls the F-M locus, provides resistance to mealiness in nonmelting flesh fruit. Only fruit with melting flesh can develop mealiness if the tree possesses other genetic susceptibility factors and/or experiences inducing conditions. The F-M locus also greatly influences susceptibility to flesh bleeding, although the physiological mechanism for this disorder is unclear and may be controlled by a different gene closely linked to endoPG. Unlike mealiness, flesh bleeding occurred primarily in non-melting flesh fruit, particularly when the fruit is white-fleshed. Flesh browning incidence was greater in mealy fruit and was not associated with flesh bleeding. Breeding for CI resistance is thus a viable long-term strategy to reduce losses in the fresh and processed peach and nectarine industries. This study is an important first step to understanding genetic control of CI symptoms in peach.

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Nonmelting-flesh Trait in Peaches Is Not Related to Low Ethylene Production Rates

Cited by 20 publications

References 14 publications

Diverse metabolism of cell wall components of melting and non‐melting peach genotypes during ripening after harvest or cold storage

Diverse metabolism of cell wall components of melting and non‐melting peach genotypes during ripening after harvest or cold storage

Cinnamyl alcohol dehydrogenases in the mesocarp of ripening fruit of Prunus persica genotypes with different flesh characteristics: changes in activity and protein and transcript levels

Influence of year and genetic factors on chilling injury susceptibility in peach (Prunus persica (L.) Batsch)

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