Nonredundant functions of <i>Arabidopsis</i> Lec<scp>RK</scp>‐V.2 and Lec<scp>RK</scp>‐<scp>VII</scp>.1 in controlling stomatal immunity and jasmonate‐mediated stomatal closure

Yekondi, Shweta; Liang, Fu Chun; Okuma, Eiji; Radziejwoski, Amandine; Wei, Hsien; Swain, Swadhin; Singh, Prashant; Gauthier, Mathieu; Chien, Hsiao Chiao; Murata, Yoshiyuki; Zimmerli, Laurent

doi:10.1111/nph.14953

Cited by 26 publications

(18 citation statements)

References 72 publications

(136 reference statements)

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“…or Alternaria brassicicola (Wang et al, 2014). LecRK-V.2, -V.5, -VI.2, -VII.1, and -IX.2 modulate PTI responses (Desclos-Theveniau et al, 2012; Singh et al, 2012; Luo et al, 2017; Yekondi et al, 2018). In rice, a cluster of three G-type LecRKs confers resistance to the phloem-sucking brown planthopper (Liu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis Lectin Receptor Kinase LecRK-I.8 Is Involved in Insect Egg Perception

et al. 2019

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Plants induce defense responses after insect egg deposition, but very little is known about the perception mechanisms. In Arabidopsis thaliana , eggs of the specialist insect Pieris brassicae trigger accumulation of reactive oxygen species (ROS) and salicylic acid (SA), followed by induction of defense genes and localized necrosis. Here, the involvement of the clade I L-type lectin receptor kinase LecRK-I.8 in these responses was studied. Expression of LecRK-I.8 was upregulated at the site of P. brassicae oviposition and egg extract (EE) treatment. ROS, SA, cell death, and expression of PR1 were substantially reduced in the Arabidopsis knock-out mutant lecrk-I.8 after EE treatment. In addition, EE-induced systemic resistance against Pseudomonas syringae was abolished in lecrk-I.8 . Expression of ten clade I homologs of LecRK-I.8 was also induced by EE treatment, but single mutants displayed only weak alteration of EE-induced PR1 expression. These results demonstrate that LecRK-I.8 is an early component of egg perception.

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Section: Discussionmentioning

confidence: 99%

The Arabidopsis Lectin Receptor Kinase LecRK-I.8 Is Involved in Insect Egg Perception

et al. 2019

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“…Kunkel (Washington University, St. Louis, Missouri, USA). Pst DC3000 bacteria were grown at 28°C in King's B medium supplemented with 50 mg/L rifampicin (Yekondi et al, 2017), and supplemented with 50 mg/L rifampicin and 50 mg/L kanamycin for Pst DC3000 (avrRpt2) bacteria.…”

Section: Plant and Pathogen Materialsmentioning

confidence: 99%

“…Chen (National Taiwan University, Taipei, Taiwan). B. cinerea was grown at room temperature on potato dextrose agar (PDB)agar plates as previously described (Zimmerli et al, 2001;Yekondi et al, 2017).…”

Section: Plant and Pathogen Materialsmentioning

confidence: 99%

The Histone Demethylase IBM1 Positively Regulates Arabidopsis Immunity by Control of Defense Gene Expression

Chan

Zimmerli

2019

Front. Plant Sci.

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Epigenetic modifications involve complex and sophisticated control over chromatin states and DNA methylation patterns, which are important for stress tolerance in plants. While the identification of epigenetic modulating enzymes keeps growing, such as MET1, for CG methylation; CMT3, DRM2, DRM3 for CHH methylation; and IBM1, SUVH4 for CHG methylation; the molecular roles of these regulators in specific physiological functions remain obscure. In a mutant screen, we identified IBM1 as a new player in plant immunity. The ibm1 mutants were hyper-susceptible to hemi-biotrophic bacteria Pseudomonas syringae. Accordingly, bacteria-induced up-regulation of PR1, PR2, and FRK1 defense markers was abolished in ibm1 mutants. Consistently, at the chromatin level, these defense marker genes showed enrichment of the inactivation mark, H3K9me2; while the activation mark H3K4me3 was reduced in ibm1 mutants. Immunoprecipitation of associated chromatin further demonstrated that IBM1 binds directly to the gene body of PR1, PR2, and FRK1. Taken together, these data suggest that IBM1 plays a critical role in modulating Arabidopsis immunity through direct regulation of defense gene expression. Notably, IBM1 maintains a permissive chromatin environment to ensure proper induction of defense genes under some biotic stress.

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“…Besides P2K1 (LecRK-I.9), LecRK-V.2 and LecRK-VII.1 were also reported to play essential roles in regulating stomatal closure (Yekondi et al, 2018). Recently, it was reported that P2K1 is involved in modulation of jasmonic acid (JA)-mediated gene expression for plant defense responses (Tripathi et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis Lectin Receptor Kinase P2K2 Is a Second Plant Receptor for Extracellular ATP and Contributes to Innate Immunity

2020

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In animals, extracellular adenosine 5'-triphosphate (eATP) is a well-studied signaling molecule that is recognized by plasma membrane-localized P2-type purinergic receptors. However, in contrast, much less is known about purinergic signaling in plants. P2 receptors play critical roles in a variety of animal biological processes including immune system regulation. The first plant purinergic receptor, Arabidopsis P2K1 (L-type lectin receptor kinase-I.9), was shown to contribute to plant defense against bacterial, oomycete and fungal pathogens. Here, we demonstrate the isolation of a second purinergic receptor, P2K2, by complementation of an Arabidopsis p2k1 mutant. P2K2 (LecRK-I.5) has 74% amino acid similarity to P2K1. The P2K2 extracellular lectin domain binds to ATP with higher affinity than P2K1 (Kd = 44.47  15.73 nM). Interestingly, p2k2 and p2k1 p2k2 mutant plants showed increased susceptibility to the pathogen Pseudomonas syringae, with the double mutant showing a stronger phenotype. In vitro and in planta studies demonstrate that P2K2 and P2K1 interact and cross-phosphorylate upon eATP treatment. Thus, similar to animals, plants possess multiple purinergic receptors.

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Nonredundant functions of Arabidopsis LecRK‐V.2 and LecRK‐VII.1 in controlling stomatal immunity and jasmonate‐mediated stomatal closure

Cited by 26 publications

References 72 publications

The Arabidopsis Lectin Receptor Kinase LecRK-I.8 Is Involved in Insect Egg Perception

The Arabidopsis Lectin Receptor Kinase LecRK-I.8 Is Involved in Insect Egg Perception

The Histone Demethylase IBM1 Positively Regulates Arabidopsis Immunity by Control of Defense Gene Expression

Arabidopsis Lectin Receptor Kinase P2K2 Is a Second Plant Receptor for Extracellular ATP and Contributes to Innate Immunity

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