Nonthermal Dielectric Barrier Discharge (DBD) Plasma Suppresses Herpes Simplex Virus Type 1 (HSV-1) Replication in Corneal Epithelium

Alekseev, Oleg; Donovan, Kelly; Limonnik, Vladimir; Azizkhan‐Clifford, Jane

doi:10.1167/tvst.3.2.2

Cited by 47 publications

(43 citation statements)

References 41 publications

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“…The increase in virucidal effect with increasing plasma exposure time is probably due to an increase in the production of reactive species in solution, as is illustrated by the reduction of the pH with increasing treatment time. This is in agreement with the results of Alekseev et al (23), who found that reduction in the infectivity of herpes simplex virus 1 was proportional to the exposure time when the infection medium was treated with nonthermal DBD air-based plasma. A similar effect was found with this plasma source in treating Pseudomonas aeruginosa (24).…”

Section: Discussionsupporting

confidence: 93%

“…Since previous studies reported that the biological activity of CGP is multifactorial and highly dependent on plasma type and exposure parameters (6,23,40), we studied the effects of several operational and exposure parameters affecting the reactivity of the resultant cold plasma and its virucidal activity in order to reach the objective of complete inactivation of FCV in solution. The effects of the plasma generation power, feed gas mixtures, exposure time, exposure distance, and virus suspension medium were studied.…”

Section: Discussionmentioning

confidence: 99%

“…Zimmermann et al (22) achieved up to 6.0 log 10 unit inactivation of adenovirus using a CGP system. Alekseev et al (23) suppressed corneal herpes simplex virus 1 infection in vitro using a nonthermal DBD plasma system. To date, no published study is available on the efficacy of CGP against foodborne viruses, such as NV and HAV.…”

mentioning

confidence: 99%

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Virucidal Effect of Cold Atmospheric Gaseous Plasma on Feline Calicivirus, a Surrogate for Human Norovirus

Aboubakr

Williams

Gangal

et al. 2015

Appl Environ Microbiol

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Minimal food-processing methods are not effective against foodborne viruses, such as human norovirus (NV). It is important, therefore, to explore novel nonthermal technologies for decontamination of foods eaten fresh, minimally processed and ready-to-eat foods, and food contact surfaces. We studied the in vitro virucidal activity of cold atmospheric gaseous plasma (CGP) against feline calicivirus (FCV), a surrogate of NV. Factors affecting the virucidal activity of CGP (a socalled radio frequency atmospheric pressure plasma jet) were the plasma generation power, the exposure time and distance, the plasma feed gas mixture, and the virus suspension medium. Exposure to 2.5-W argon (Ar) plasma caused a 5.55 log 10 unit reduction in the FCV titer within 120 s. The reduction in the virus titer increased with increasing exposure time and decreasing exposure distance. Of the four plasma gas mixtures studied (Ar, Ar plus 1% O 2 , Ar plus 1% dry air, and Ar plus 0.27% water), Ar plus 1% O 2 plasma treatment had the highest virucidal effect: more than 6.0 log 10 units of the virus after 15 s of exposure. The lowest virus reduction was observed with Ar plus 0.27% water plasma treatment (5 log 10 unit reduction after 120 s). The highest reduction in titer was observed when the virus was suspended in distilled water. Changes in temperature and pH and formation of H 2 O 2 were not responsible for the virucidal effect of plasma. The oxidation of viral capsid proteins by plasma-produced reactive oxygen and nitrogen species in the solution was thought to be responsible for the virucidal effect. In conclusion, CGP exhibits virucidal activity in vitro and has the potential to combat viral contamination in foods and on food preparation surfaces. Foodborne illnesses continue to plague public health, as well as world economies, costing approximately $152 billion in the United States alone (1). Enteric viruses, particularly human norovirus (NV) and hepatitis A virus (HAV), are the leading causes of viral foodborne illnesses (2). Human NV, one of the top five pathogens with respect to the total cost of foodborne illnesses in the United States, belongs to the family Caliciviridae and is a wellknown cause of "winter vomiting disease" or "stomach flu" (3). NV causes 19 to 21 million cases of acute gastroenteritis annually in the United States and leads to 1.7 to 1.9 million outpatient visits, 400,000 emergency room visits, 56,000 to 71,000 hospitalizations, and 570 to 800 deaths, mostly among young children (4). More than half of all foodborne disease outbreaks due to a known cause reported to the CDC from 2006 to 2010 were attributed to NV. In the European Union in 2007, caliciviruses (primarily NV) were responsible for 507 of 675 foodborne viral disease outbreaks (5).Multiple issues related to the quality of thermally processed foods, e.g., nutritional losses and adverse effects on organoleptic quality, have led to the emergence of so-called nonthermal technologies, which consist of preservation treatments that are effective at ambient o...

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Virucidal Effect of Cold Atmospheric Gaseous Plasma on Feline Calicivirus, a Surrogate for Human Norovirus

Aboubakr

Williams

Gangal

et al. 2015

Appl Environ Microbiol

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“…CAP has a complex chemical composition and some of the charged particles detected in the CAP, such as NO - , NO 2 - , OH - , O - , as well as reactive oxygen species (O 3, O), have important biological and biomedical activities. The ongoing research suggests that CAP can be used for decontamination and sterilization, and appears to be quite effective in virus inactivation [1–4]. The mechanisms behind the anti-viral activity of CAP are poorly understood, but may include both a direct effect on viral particles via bioactive ions and indirect suppression via modification of cell surface receptors important for viral replication [5].…”

Section: Introductionmentioning

confidence: 99%

Cold Atmospheric Plasma Inhibits HIV-1 Replication in Macrophages by Targeting Both the Virus and the Cells

et al. 2016

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Cold atmospheric plasma (CAP) is a specific type of partially ionized gas that is less than 104°F at the point of application. It was recently shown that CAP can be used for decontamination and sterilization, as well as anti-cancer treatment. Here, we investigated the effects of CAP on HIV-1 replication in monocyte-derived macrophages (MDM). We demonstrate that pre-treatment of MDM with CAP reduced levels of CD4 and CCR5, inhibiting virus-cell fusion, viral reverse transcription and integration. In addition, CAP pre-treatment affected cellular factors required for post-entry events, as replication of VSV-G-pseudotyped HIV-1, which by-passes HIV receptor-mediated fusion at the plasma membrane during entry, was also inhibited. Interestingly, virus particles produced by CAP-treated cells had reduced infectivity, suggesting that the inhibitory effect of CAP extended to the second cycle of infection. These results demonstrate that anti-HIV activity of CAP involves the effects on target cells and the virus, and suggest that CAP may be considered for potential application as an anti-HIV treatment.

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“…However, the plasma produced at the higher pulse rate also generates greater/cytotoxic concentrations of ROS and RNS (Liu et al, 2014;Zhang et al, 2014). To date, there has been a lack of studies characterizing each plasma type and condition, which has led to a disconnect in determining how the different plasmas affect biological tissues and cellular processes (Jiang et al, 2009;Duval et al, 2013;Alekseev et al, 2014;Li et al, 2014). To further confound our understanding, isolated cells treated with plasma also tend to react much differently than treated tissues (unpublished data).…”

Section: Introductionmentioning

confidence: 99%

Chemical modification of extracellular matrix by cold atmospheric plasma-generated reactive species affects chondrogenesis and bone formation

Eisenhauer

Chernets

Song

et al. 2016

J Tissue Eng Regen Med

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The goal of this study was to investigate whether cold plasma generated by dielectric barrier discharge (DBD) modifies extracellular matrices (ECM) to influence chondrogenesis and endochondral ossification. Replacement of cartilage by bone during endochondral ossification is essential in fetal skeletal development, bone growth and fracture healing. Regulation of this process by the ECM occurs through matrix remodelling, involving a variety of cell attachment molecules and growth factors, which influence cell morphology and protein expression. The commercially available ECM, Matrigel, was treated with microsecond or nanosecond pulsed (µsp or nsp, respectively) DBD frequencies conditions at the equivalent frequencies (1 kHz) or power (~1 W). Recombinant human bone morphogenetic protein-2 was added and the mixture subcutaneously injected into mice to simulate ectopic endochondral ossification. Two weeks later, the masses were extracted and analysed by microcomputed tomography. A significant increase in bone formation was observed in Matrigel treated with µsp DBD compared with control, while a significant decrease in bone formation was observed for both nsp treatments. Histological and immunohistochemical analysis showed Matrigel treated with µsp plasma increased the number of invading cells, the amount of vascular endothelial growth factor and chondrogenesis while the opposite was true for Matrigel treated with nsp plasma. In support of the in vivo Matrigel study, 10 T1/2 cells cultured in vitro on µsp DBD-treated type I collagen showed increased expression of adhesion proteins and activation of survival pathways, which decreased with nsp plasma treatments. These results indicate DBD modification of ECM can influence cellular behaviours to accelerate or inhibit chondrogenesis and endochondral ossification.

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Nonthermal Dielectric Barrier Discharge (DBD) Plasma Suppresses Herpes Simplex Virus Type 1 (HSV-1) Replication in Corneal Epithelium

Cited by 47 publications

References 41 publications

Virucidal Effect of Cold Atmospheric Gaseous Plasma on Feline Calicivirus, a Surrogate for Human Norovirus

Virucidal Effect of Cold Atmospheric Gaseous Plasma on Feline Calicivirus, a Surrogate for Human Norovirus

Cold Atmospheric Plasma Inhibits HIV-1 Replication in Macrophages by Targeting Both the Virus and the Cells

Chemical modification of extracellular matrix by cold atmospheric plasma-generated reactive species affects chondrogenesis and bone formation

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