Nontypeable Haemophilus influenzae newly released (NRel) from biofilms by antibody-mediated dispersal versus antibody-mediated disruption are phenotypically distinct

Mokrzan, Elaine M.; Ahearn, Christian P.; Buzzo, John R.; Novotny, Laura A.; Zhang, Yan; Goodman, Steven D.

doi:10.1016/j.bioflm.2020.100039

Cited by 20 publications

(37 citation statements)

References 104 publications

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“…We have previously shown that these proteins also serve a role extracellularly, wherein they act as linchpin proteins to stabilize the crossed-strand structure of eDNA, and these eDNA structures functionally resemble Holliday junctions (HJs) (Devaraj et al, 2019) within the biofilm EPS and are required for the stability of the biofilm matrix (Brockson et al, 2014;Devaraj et al, 2018). Targeted removal of DNABII proteins with specific antibodies results in rapid, significant biofilm collapse (Devaraj et al, 2015;Gunn et al, 2016;Novotny et al, 2013a;Rocco et al, 2018) with release of resident bacteria that are markedly more susceptible to antibiotics and host immune effectors (Brockson et al, 2014;Gunn et al, 2016;Mokrzan et al, 2020a;Novotny et al, 2013a).…”

Section: Introductionmentioning

confidence: 99%

Z-form extracellular DNA is a structural component of the bacterial biofilm matrix

Buzzo

Devaraj

Gloag

et al. 2021

Cell

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122

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Section: Introductionmentioning

confidence: 99%

Z-form extracellular DNA is a structural component of the bacterial biofilm matrix

Buzzo

Devaraj

Gloag

et al. 2021

Cell

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122

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“…Proteomic profiling was also useful to prove that the two bacterial populations released from a NTHi biofilm by using antibodies directed against the type IV pilus or, alternatively, against a DNABII DNA-binding protein, are different not only from planktonically grown NTHi, but also from each other despite genetic identity. Even more, each newly released population had a distinct, increased susceptibility to antibiotic killing compared to planktonic bacteria, highlighting new clues for anti-biofilm therapeutics [93] .…”

Section: Update On H Influenzae Protein and Metabolite Profilingmentioning

confidence: 99%

“…Dual RNA-seq gene expression profiling [87] D. Proteomic profiling D1. Bacterial responses to changing environmental conditions [89] , [91] , [92] , [93] D2. Bacterial responses to gene inactivation [54] , [55] , [81] E. Metabolomic profiling E1.…”

Section: Introductionmentioning

confidence: 99%

Learning from –omics strategies applied to uncover Haemophilus influenzae host-pathogen interactions: Current status and perspectives

López-López

Gil-Campillo

Díez-Martínez³

et al. 2021

Computational and Structural Biotechnology Journal

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Haemophilus influenzae has contributed to key bacterial genome sequencing hallmarks, as being not only the first bacterium to be genome-sequenced, but also starring the first genome-wide analysis of chromosomes directly transformed with DNA from a divergent genotype, and pioneering Tn-seq methodologies. Over the years, the phenomenal and constantly evolving development of –omic technologies applied to a whole range of biological questions of clinical relevance in the H. influenzae -host interplay, has greatly moved forward our understanding of this human-adapted pathogen, responsible for multiple acute and chronic infections of the respiratory tract. In this way, essential genes, virulence factors, pathoadaptive traits, and multi-layer gene expression regulatory networks with both genomic and epigenomic complexity levels are being elucidated. Likewise, the unstoppable increasing whole genome sequencing information underpinning H. influenzae great genomic plasticity, mainly when referring to non-capsulated strains, poses major challenges to understand the genomic basis of clinically relevant phenotypes and even more, to clearly highlight potential targets of clinical interest for diagnostic, therapeutic or vaccine development. We review here how genomic, transcriptomic, proteomic and metabolomic-based approaches are great contributors to our current understanding of the interactions between H. influenzae and the human airways, and point possible strategies to maximize their usefulness in the context of biomedical research and clinical needs on this human-adapted bacterial pathogen.

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“…Otopathogens released from the destabilised biofilm are highly susceptible to antibiotics 19 . The PilA protein is included in a trivalent sub-unit NTHi vaccine that was tested in clinical trials in adults with chronic lung disease 20 .…”

Section: Anti-biofilm Treatmentsmentioning

confidence: 99%

Targeting host-microbial interactions to develop otitis media therapies

Kirkham

Thornton

2021

Microbiol. Aust.

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Otitis media (OM; middle ear infection) is the most common reason for pre-school children to visit a doctor, be prescribed antimicrobials, or undergo surgery. Recent Cochrane reviews of clinical trials have identified that antibiotics and grommet surgery are only moderately effective in treating OM, with recurrent or persistent infection observed in one-third of children. Research efforts are focusing on developing improved therapies to treat OM and prevent disease recurrence. The recurrent nature of OM is mostly due to the persistence of bacterial pathogens within established biofilm in the middle ear. Promising novel therapies are harnessing host-microbe interactions to disrupt middle ear biofilm and permit antibiotics to work more effectively. New approaches are also being developed to prevent OM, including new vaccines and mining the host respiratory microbiome to develop novel bacterial therapies. This review describes how our improved knowledge of human and microbial interactions is driving development of OM therapies to improve health outcomes for children in Australia and worldwide.

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Nontypeable Haemophilus influenzae newly released (NRel) from biofilms by antibody-mediated dispersal versus antibody-mediated disruption are phenotypically distinct

Cited by 20 publications

References 104 publications

Z-form extracellular DNA is a structural component of the bacterial biofilm matrix

Z-form extracellular DNA is a structural component of the bacterial biofilm matrix

Learning from –omics strategies applied to uncover Haemophilus influenzae host-pathogen interactions: Current status and perspectives

Targeting host-microbial interactions to develop otitis media therapies

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