Nonviral Vectors for Gene Delivery

Mintzer, Meredith A.; Simanek, Eric E.

doi:10.1021/cr800409e

Cited by 2,188 publications

(1,986 citation statements)

References 563 publications

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“…The transfection process can be affected by many unknown factors. However, several studies have linked the transfection efficiency and cytotoxicity of PEI preparations to the physicochemical properties, the molecular weight and the branching ratio of polymer [41,42], the amount of DNA, the DNA ratio to the PEI, the timing and the solution conditions for complex formation, the transfection medium and cell density at time of transfer [43,44]. In addition, many factors, including temperature, surfactant, complex concentration, ionic strength, viscosity, pH, can significantly affect the aggregation process.…”

Section: Peimentioning

confidence: 99%

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Zakeri

Kouhbanani

Beheshtkhoo

et al. 2018

Nano Reviews & Experiments

235

150

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The meaning of gene therapy is the delivery of DNA or RNA to cells for the treatment or prevention of genetic disorders. The success rate of gene therapy depends on the progression and safe gene delivery system. The vectors available for gene therapy are divided into viral and non-viral systems. Viral vectors cause higher transmission efficiency and long gene expression, but they have major problems, such as immunogenicity, carcinogenicity, the inability to transfer large size genes and high costs. Non-viral gene transfer vectors have attracted more attention because they exhibit less toxicity and the ability to transfer large size genes. However, the clinical application of non-viral methods still faces some limitations, including low transmission efficiency and poor gene expression. In recent years, numerous methods and gene-carriers have been developed to improve gene transfer efficiency. The use of Polyethylenimine (PEI) based transfer of collaboration may create a new way of treating diseases and the combination of chemotherapy and gene therapy. The purpose of this paper is to introduce the PEI as an appropriate vector for the effective gene delivery.

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Section: Peimentioning

confidence: 99%

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Zakeri

Kouhbanani

Beheshtkhoo

et al. 2018

Nano Reviews & Experiments

235

150

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“…However, viral gene delivery has significant drawbacks restricting clinical use including immunogenicity, toxicity and technical challenges for large scale production [18][19][20].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy

Fernandes

Chari

2016

Journal of Controlled Release

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Please cite this article as: Alinda R. Fernandes, Divya M. Chari, Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy, Journal of Controlled Release (2016Release ( ), doi: 10.1016Release ( /j.jconrel.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT

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“…Non-viral vectors, such as cationic lipids, polymers, dendrimers and peptides, have recently attracted a great deal of attention because of their ease in preparation, flexibility in the size of the transgene and biocompatibility. [1][2][3][4] Polyethyleneimine (PEI) is a polymeric non-viral vector that has shown promise to effectively condense nucleic acids into nanosize particles, facilitate their escape from endosomal compartments and mediate gene expression in a variety of mammalian cells. [5][6][7] The endosomolytic activity of PEI is proportional to its molecular weight and, as it increases, the ability to lyse endosomes increases.…”

Section: Introductionmentioning

confidence: 99%

Modified polyethyleneimine with histidine–lysine short peptides as gene carrier

et al. 2010

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There are several strategies that can be utilized to improve transfection efficiency while reducing the cytotoxicity of polyethyleneimine (PEI) as a promising non-viral gene delivery vector. In this study, we evaluated the potential use of lysinehistidine (KH) peptides in modifying the PEI 10 kDa structure and enhancing its efficiency while maintaining low toxicity of PEI. PEI 10 kDa was modified with 6-bromohexanoic acid (alkyl) to increase its lipophilicity. Then, ethylenediamine (EDA) was attached to the carboxylic groups of PEI-hexanoate to restore the primary amines of PEI. Subsequently, six different KH short peptides were conjugated to PEIs and evaluated for the effect of the KH sequence on vector transfection efficiency and cytotoxicity. The transfection efficiency of PEI-peptides complexed with a luciferase reporter gene (pRLCMV) in Neuro-2A murine neuroblastoma cells showed that the PEI conjugated to KHHHKKHHHK peptide had a significantly higher rate of gene transfection efficiency in comparison with other KH peptides. This peptide was conjugated to PEI-alkyl and PEI-alkyl-EDA and significant improvement in efficiency with minimal cytotoxicity was observed. The results obtained suggest that the sequence and content of KH peptides will have a significant impact on the transfection efficiency of modified PEI 10 kDa.

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Nonviral Vectors for Gene Delivery

Cited by 2,188 publications

References 563 publications

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy

Modified polyethyleneimine with histidine–lysine short peptides as gene carrier

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