Nonwoven polycaprolactone scaffolds for tissue engineering: the choice of the structure and the method of cell seeding

Arutyunyan, I. V.; Тенчурин, Т. Х.; Kananykhina, Evgeniya; Chernikov, V. V.; Vasyukova, Vasyukova O.A.; Elchaninov, Andrey; Макаров, А. В.; Korshunov, A.S.; Burov, A.; Podurovskaya, Y.; Чупрынин, В.Д.; Uvarova, E. A.; Degtyarev, D.; Шепелев, А. Д.; Мамагулашвили, В. Г.; Kamyshinskiy, Roman V.; Krasheninnikov, S. V.; Chvalun, S.; Fatkhudinov, Timur

doi:10.23868/201703009

Cited by 3 publications

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“…Of equal importance are the method of the non-woven material population and potential ways of improving matrix functional properties using additional treatment (for example, enrichment with collagen) [14,15]. The choice of the method of synthetic material population is known to depend on electroforming conditions determining the structural and mechanical properties of the created matrices and the required parameters [7,16].…”

Section: Discussionmentioning

confidence: 99%

“…The majority of plastic operations are performed using synthetic materials based on polytetrafluoroethylene, polyethylene, and polypropylene (AlloDerm, Gore-Tex, Marlex) which lack bioresorbability and, therefore, promote the recurrence of the pathological process [1,4]. Non-woven biodegradable polycaprolactonebased materials are considered by many authors the most perspective for replacing the defects of the skin, bone tissue, small vessels, and diaphragm [5][6][7][8]. At the same time, polylactide, being an inexpensive material, possesses a required strength (greater than polycaprolactone), biocompatibility, biodegradability without toxic metabolites making it rather promising for fabricating implants and non-woven materials necessary in regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

“…The majority of the investigations performed [8] are focused on the study of biomechanical and bioresorptive properties of the non-woven materials, the choice of optimal way of implant population in vitro. And a cellular component is considered as an additional factor promoting the replacement of the prosthetic area by the autologous connective tissue of the recipient [7] without adequate reconstruction of the anatomical and physiological properties of the native organ. Implantation of recellularized synthetic matrix into the animal organism and subsequent examination of the efficiency of the tissue defect replacement, cell growth, and MSC proliferation on the synthetic matrix as a stage of TEC creation is an interesting but insufficiently studied task [2].…”

Section: Introductionmentioning

confidence: 99%

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Application of Recellularized Non-Woven Materials from Collagen-Enriched Polylactide for Creation of Tissue-Engineered Diaphragm Constructs

Куевда¹,

Губарева²,

Grigoriev³

et al. 2019

Sovrem Tehnol Med

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The aim of the investigation was to study biocompatibility and biomechanical properties of recellularized non-woven materials based on collagen-filled polylactide under in vitro and in vivo conditions and to assess the potential of their use for diaphragm defect replacement in the experiment on small laboratory animals. Materials and Methods. Non-woven materials were obtained by electroforming of polylactide in a tight box with subsequent specimen vacuumization. 9% aqueous solution of polylactide was prepared in the solvent mixture: chemically pure chloroform with 10% addition of chemically pure ethanol. The microfibrous material was enriched with collagen in a weight concentration of 0.1 and 0.5% with subsequent freezing at-40° or in liquid nitrogen. The structure of non-woven polylactide matrices was assessed by a scanning electron microscopy while mechanical properties were evaluated during cyclic tests for strength and fatigue properties using tensile-testing machine. The fabricated non-woven matrices were recellularized by mesenchymal stromal cells under static conditions. The quality of the fabricated tissue-engineered constructs was evaluated morphologically by routine histological investigations. The viability of the cells on the matrices and the cytotoxic properties of the matrices were determined using XTT tests. The least toxic specimens were orthotopically implanted into the rats with subsequent morphological examinations. Results. The morphological analysis and viability study of the cells on the matrices of those specimens which underwent freezing in liquid nitrogen during forming were found to be inexpedient for orthotopic transplantation. The specimens frozen in the refrigeration unit demonstrated permissible cytotoxicity levels and were implanted. The morphological examination after explantation of the given specimen groups did not reveal significant damage to the structure and inflammatory changes though the signs of the marked adhesive process in the implantation area were evident. Conclusion. In spite of the ability of the non-woven matrices to stimulate fibrinogenesis, absence of cytotoxic properties and evident reactions of transplant rejection allow the non-woven polylactide matrices undergone freezing in the refrigeration unit at-40° during the forming process to be considered promising for creation of tissue-engineered constructs of a diaphragm.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Application of Recellularized Non-Woven Materials from Collagen-Enriched Polylactide for Creation of Tissue-Engineered Diaphragm Constructs

Куевда¹,

Губарева²,

Grigoriev³

et al. 2019

Sovrem Tehnol Med

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Angiogenic vitalization of biocompatible and biodegradable scaffold ( experimental study)

Klabukov¹,

Балясин²,

Люндуп³

et al. 2018

ZHurnal «Patologicheskaia Fiziologiia I Eksperimental`naia Terapiia»

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Цель исследования - оценка влияния на ангиогенез конструкций из волокнистого поликапролактона, модифицированного плазмидой с геном сосудистого фактора роста, при имплантации крысам. Методика. Эксперименты выполнены на 24 крысах-самках Вистар в возрасте 2 мес, массой 180-200 г. В работе исследовали плоские каркасы размером 1 см х 1 см, полученные методом эмульсионного электроспиннинга из раствора поликапролактона. Материал каркасов витализировали плазмидой VEGF-165 (геннотерапевтический препарат Неоваскулген), введенной внутрь двух типов волокнистых материалов в разных концентрациях: низкой - 0,005 мг/мл, и высокой - 0,05 мг/мл. Образец и контроль (материал без витализации) одномоментно имплантировали подкожно в два сформированных симметричных кармана в межлопаточной зоне. Окружающие каркас ткани на 7-е, 16-е, 33-и, 46-е и 64-е сутки извлекали, проводили гистологическое исследование: изучали тканевую реакцию с морфометрической оценкой плотности распределения и диаметра сосудов в области имплантации, а также оценивали степень биодеградации волокнистого материала. Результаты. Признаков тканевой реакции отторжения при имплантации как контрольного, так и модифицированного материала не выявлено. Показано, что при экспозиции материала in vivo наряду с резорбцией материала происходят изменения количества и диаметра сосудов. Выявлен дозозависимый эффект стимуляции ангиогенеза при увеличении концентрации Неоваскулгена в образцах. Для витализированных материалов отмечено увеличение плотности распределения сосудов на 46% (высокая концентрация, 33-и сут) по сравнению с контролем. После прекращения воздействия препарата, плотность распределения сосудов приближалась к значениям в контроле. Заключение. Разработанная методика витализации полимерных каркасов с внесением раствора геннотерапевтического препарата Неоваскулген внутрь микроволокон обеспечивает пролонгированный и дозозависимый эффект на рост сосудов в зоне имплантации. The aim of the study was to evaluate the effect on angiogenesis of a biocompatible, biodegradable material-derived scaffold implanted into rats and functionalized using a plasmid with a vascular growth factor gene. Methods. Experiments were performed on 24 female Wistar rats aged 2 months weighing 180-200 g. We investigated 1 cm x 1 cm flat scaffolds obtained by electrospinning from polycaprolactone functionalized scaffolds with a VEGF-165 plasmid (gene therapy drug, Neovasculgen) incorporated inside the fibers at two concentrations, low (0.005 mg/ml) and high (0.05 mg/ml). The sample and control were simultaneously implanted subcutaneously into two formed symmetrical pockets in the interblade zone. At 7, 16, 33, 46, and 64 days, the scaffolds were removed, and histological examination was performed; the tissue reaction was studied including morphometric evaluation of density and diameter of blood vessels in the implantation area, and the area of the image occupied by the material was measured. Results. Tissue rejection was absent after implantation of either control or modified material. When the material was exposed in vivo , besides resorption of the material, blood vessel number and diameter changed. As the Neovasculgen concentration in samples increased, a dose-dependent effect of angiogenesis stimulation became evident. Vascular density was increased by 46% (high concentration, 33 days) in functionalized matrices compared to the control. After cessation of the drug treatment, the vascular density approached the control values. Conclusion. The developed technique for functionalizing polymeric scaffolds by administration of a solution of the gene therapy drug, Neovasculgen, into microfibers provides a prolonged and dose-dependent effect on growth of blood vessels in the implantation zone.

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Use of biodegradable polycaprolactone matrix for filling bone defects (experimental study)

Попков

Горбач

Кононович

et al. 2022

Acta biomedica scientifica

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Background. There are unresolved issues in bone defect management associated with complications, invasiveness and long duration of treatment. The use of elastic implants made of bioactive biodegradable materials that take any form of defect could close many of them.The aim. To investigate features of reparative regeneration in filling bone defects with an elastic degradable implant made of polycaprolactone (PCL) with and without hydroxyapatite (HA).Materials and methods. The study was carried out on 10 adult mongrel dogs. A non-through cylindrical hole, 4 mm in diameter and 10 mm deep, was modeled in the upper third of the diaphysis of the tibia. The defects thus formed were filled with an elastic degradable implant made of polycaprolactone. In Group 1, HA was not added to polycaprolactone, while HA was added in dogs of Group 2. Radiographic and histological methods were used to study the results.Results. It was found that the tested materials did not cause toxic and allergic reactions, both local and general, during intravital observations and in post-mortem anatomical preparations. After 28 days in both series, the implant biodegraded and was replaced by bone tissue. The proportion of the bone component and the numerical density of microvessels in the defect zone in Group 2 were significantly higher than in Group 1.Conclusion. Elastic implants produced of polycaprolactone by electrospinning are biologically compatible, biodegradable and can be used to heal bone defects. Hydroxyapatite that was added stimulates the activity of osteogenesis.

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Nonwoven polycaprolactone scaffolds for tissue engineering: the choice of the structure and the method of cell seeding

Cited by 3 publications

References 0 publications

Application of Recellularized Non-Woven Materials from Collagen-Enriched Polylactide for Creation of Tissue-Engineered Diaphragm Constructs

Application of Recellularized Non-Woven Materials from Collagen-Enriched Polylactide for Creation of Tissue-Engineered Diaphragm Constructs

Angiogenic vitalization of biocompatible and biodegradable scaffold ( experimental study)

Use of biodegradable polycaprolactone matrix for filling bone defects (experimental study)

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