NOR Polymorphism and Chromosome Analysis of Banggai Cardinalfish, <i>Pterapogon kauderni</i> (Perciformes, Apogonidae)

Abstract

This is the first nucleolar organizer region (NOR) polymorphism and chromosome analysis of Banggai cardinalfish (Pterapogon kauderni Koumans, 1933). Kidney cell samples were taken from 10 male and 10 female fish. Mitotic chromosome preparations were prepared directly from kidney cells. Conventional and Ag-NOR banding techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of P. kauderni was 2n=46, and the fundamental number (NF) was 92 in both males and females. The types of chromosomes were 6 large acrocentric, 4 medium metacentric, 14 medium submetacentric, and 22 medium acrocentric chromosomes. The results indicated that the short arm subtelomeric of the acrocentric chromosome pair 13 showed clearly observable NORs. This finding exhibited that three NOR polymorphism patterns were found: 1) homomorphic which shows an equal size of both chromosome pair 13 (13a13a), 2) heteromorphic that displays different sizes of NORs of chromosome pair 13 (13a13c) and 3) heteromorphic which is found in only one homologous chromosome pair 13 (13a13b). There was no observation of strange size chromosomes related to sex. The karyotype formula for P. kauderni was: a m sm a 6 4 14 22 2 (diploid) 46 = L + M + M + M n

First cytogenetic characterization of the Amazon Catfish Leiarius marmoratus (Gill, 1870) and its hybrid with Pseudoplatystoma reticulatum (Eigenmann & Eigenmann, 1889)

“…Variability in the karyotype formula without changes on the diploid number is a common feature in the Pimelodidae family, also verified for other Pseudoplatystoma species (Porto- Foresti et al 2000;Nirchio et al 2003) and among the Pimelodidae family (Swarça et al 2000), which can be explained by structural chromosomal rearrangements as pericentric inversions during their evolution and speciation events (Swarça et al 2000; A polymorphism of Ag-NOR marks between the homologous chromosomes were detected for L. marmoratus and P. reticulatum in this study (Fig. 1A, 1C -boxes), which is a relatively common feature observed for several groups of fishes including Characiformes (Vicari et al 2006), Cypriniformes (Supiwong et al 2012), Siluriformes (Swarça et al 2005;Prado et al 2012) and others fishes (Kasiroek et al 2017). Differences in NOR size have been attributed to structural events such as chromosomal breaks, duplications of the ribosomal DNA clusters or differences in NOR activity.…”

History and New Developments on the 90th Anniversary of Cytologia: On the Occasion of the Publication of “Dawn of Modern Wheat Genetics”

“…In Thailand, including Indochina and the northern Malay peninsula, Alongklod Tanomtong and coworkers have recently focused on fish karyotype analysis and have published 15 reports in Cytologia over the last 2 years on the following fish species: Humpback cardinalfish, Fibramia lateralis (Kasiroek et al 2017a); Banggai cardinalfish, Pterapogon kauderni (Kasiroek et al 2017b); triangle butterflyfish, Chaetodon triangulum and yellow butterflyfish, C. andamanensis (Supiwong et al 2017a); Indian vagabond butterflyfish, Chaetodon decussatus and Lined butterflyfish, C. lineolatus (Supiwong et al 2017b); Nile tilapia, Oreochromis niloticus (Sriuttha et al 2017); three-spot damselfish, Dascyllus trimaculatus (Getlekha et al 2017); ocellated dragonet, Synchiropus ocellatus and picturesque dragonet, S. picturatus (Kasiroek et al 2017c); crystal eye catfish, Hemibagrus wyckii (Supiwong et al 2017d); Chevey s sheetfish, Micronema cheveyi (Pinthong et al 2017); F 1 hybrid catfish: Mekong giant catfish, Pangasianodon gigas striped catfish, P. hypophthalmus and spot pangasius, Pangasius larnaudii Pangasianodon hypophthalmus (Sreeputhorn et al 2017); whitecheek monocle bream, Scolopsis vosmeri (Supiwong et al 2017c); snakehead fishes, Channa marulius and C. marulioides (Sarasan et al 2018); Hihgfin barb fish, Cyclocheilichthys armatus (Chaiyasan et al 2018); black lancer, Bagrichthys majusculus (Supiwong et al 2018); and longfin carp, Labiobarbus leptocheilus (Saenjundaeng et al 2018).…”

Nucleolar Organizer Regions Polymorphism and Karyological Analysis of Black Lancer, <i>Bagrichthys majusculus</i> (Siluriformes, Bagridae) in Thailand

“…This intraspecific NOR polymorphism is common event found previously in several fishes in Moenkhausia sanctaefilomenae (Foresti et al 1989), Aphanius fasciatus (Vitturi et al 1995), Leporinus friderici (Galetti et al 1995), Salmo trutta (Castro et al 1996), Salvelinus alpines (Reed and Phillips 1997), Chondrostoma lusitanicum (Rodrigues andCollares-Pereira 1996, Collares-Pereira andRáb 1999), Hoplias malabaricus (Born and Bertollo 2000), Oedalechilus labeo (Rossi et al 2000), Astyanax scabripinnis (Mantovani et al 2000, Marco-Ferro et al 2001, Soza et al 2001, A. altiparanae (Pacheco et al 2001, Mantovani et al 2005, Brycon americus (Paintner-Marques et al 2002), Apareiodon affinis (Jorge and Filho 2004), Aphanius fasciatus (Vitturi et al 2005), Prochilodus lineatus (Gras et al 2007), Br. iheringii (Capistano et al 2008), Puntioplites proctozysron (Supiwong et al 2012), Lutjanus johnii (Phimphan et al 2013), Pterapogon kauderni (Kasiroek et al 2017) and Hemibagrus wyckii (Supiwong et al 2017). Therefore, different karyotypes are found even in small and isolated populations of these species (Capistano et al 2008).…”