Norepinephrine depresses the nitric oxide production in the ascidian hemocytes

Barros, Cíntia Monteiro de; Mello, Andressa de Abreu; Allodi, Silvana

doi:10.1016/j.jip.2012.07.002

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…Further, when comparing the expression time courses of NO and NE after LPS stimulation, it might be speculated that NE bonded with α-AR to inhibit the generation of NO during early phases of immune defense, whereas it bonded with β-AR and induced higher level of NO production in later phases. However, in acute immune responses of ascidian haemocytes, NE down-regulated NO production by coupling with either α- or β-AR26. The differed results might be partly explained by the species specificity and the disparate intracellular reactions in response to different stimuli.…”

Section: Discussionmentioning

confidence: 93%

“…But the neuroendocrine modulation of NO was seldom reported in invertebrates. As an important neuroendocrine hormone, catecholamine was able to modulate the level of NO in the ascidian haemocytes26. And the NO production could be modulated by the cell signal pathways containing protein kinase A (PKA), extracellular signal-regulated kinase (ERK) and protein kinase C (PKC) in mollusc1327.…”

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confidence: 99%

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Mutual modulation between norepinephrine and nitric oxide in haemocytes during the mollusc immune response

Zhou

Wang

Yang

et al. 2014

Sci Rep

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Nitric oxide (NO) is one of the most important immune molecules in innate immunity of invertebrates, and it can be regulated by norepinephrine in ascidian haemocytes. In the present study, the mutual modulation and underlying mechanism between norepinephrine and NO were explored in haemocytes of the scallop Chlamys farreri. After lipopolysaccharide stimulation, NO production increased to a significant level at 24 h, and norepinephrine concentration rose to remarkable levels at 3 h and 12~48 h. A significant decrease of NO production was observed in the haemocytes concomitantly stimulated with lipopolysaccharide and α-adrenoceptor agonist, while a dramatic increase of NO production was observed in the haemocytes incubated with lipopolysaccharide and β-adrenoceptor agonist. Meanwhile, the concentration of cyclic adenosine monophosphate (cAMP) decreased significantly in the haemocytes treated by lipopolysaccharide and α/β-adrenoceptor agonist, while the content of Ca2+ was elevated in those triggered by lipopolysaccharide and β-adrenoceptor agonist. When the haemocytes was incubated with NO donor, norepinephrine concentration was significantly enhanced during 1~24 h. Collectively, these results suggested that norepinephrine exerted varied effects on NO production at different immune stages via a novel α/β-adrenoceptor-cAMP/Ca2+ regulatory pattern, and NO might have a feedback effect on the synthesis of norepinephrine in the scallop haemocytes.

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Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 99%

Mutual modulation between norepinephrine and nitric oxide in haemocytes during the mollusc immune response

Zhou

Wang

Yang

et al. 2014

Sci Rep

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“…In addition to the vasoconstrictor effect, norepinephrine and vasopressin can also act by decreasing the production of nitric oxide [42,43]. Thus, in the control group, in which MB was not used, vasopressors showed a direct and strong correlation with nitrate.…”

Section: Discussionmentioning

confidence: 99%

Methylene blue therapy versus standard treatment for acute-phase septic shock: a pilot randomized controlled trial

Luis-Silva,

Menegueti,

Peres

et al. 2024

Preprint

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Purpose Methylene blue (MB) has been used to increase blood pressure in patients with septic shock by acting on guanylate cyclase and nitric oxide synthase (NOS). Objective To determine whether the administration of MB to patients in the initial phase of septic shock leads to a reduction in the use of vasopressors compared to that in the control group. Methods This was a 1:1 randomized clinical trial of two groups (methylene blue and control). We used MB after fluid replacement, vasopressors and antibiotic therapy. Patients received a loading dose of MB (3 mg/kg) and maintenance (0.5 mg/kg/h) for 48 hours. Vasopressor doses, laboratory test results, inflammatory and anti-inflammatory cytokine levels, and hemodynamic monitoring were recorded before the infusion of MB (T1) and after 20 minutes (T2), 2 hours (T3), 24 hours (T4), 48 hours after the infusion started (T5) and 24 hours after weaning (T6). Results Methylene blue therapy started within 72 hours of septic shock. The methylene blue group showed an immediate reduction in NOR dosage, earlier reduction in VAS dosage, and higher IL-10 levels compared to the control group. Integrative network analysis highlighted NO and IL-10's roles in coordinating correlations with "Hemodynamic Monitoring" in the control and methylene blue groups, respectively. Conclusion Early methylene blue (MB) administration alongside standard septic shock treatment reduces vasopressor doses, possibly involving nitric oxide (NO) mechanisms. A possible mechanism of action may involve modulation of inflammatory and anti-inflammatory mediators, enhancing immune response. However, larger and longer studies are needed for validation. Trial registration: Clinical registration: https://ensaiosclinicos.gov.br/rg/RBR-96584w4.

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“…Among the different types of blood cells of Styela plicata , the lymphocyte-like cells produce most of the NO, as revealed by NADPH-diaphorase and immunostaining with antibodies against inducible Nos [ 76 ]. Also, hemocytes of Phallusia nigra contain NO, detected using the cell-permeant 4-Amino-5-Methylamino-2′,7′-Difluorofluorescein Diacetate (DAF-FM-DA), which becomes fluorescent after reaction with NO [ 77 ]. No conclusive data were obtained by functional experiments in which NO production was investigated in hemocytes under inflammatory conditions after treatment with lipopolysaccharide (LPS) or zymosan A [ 76 , 77 ].…”

Section: No Function In Tunicatesmentioning

confidence: 99%

Nitric Oxide Function and Nitric Oxide Synthase Evolution in Aquatic Chordates

Locascio

Annona

Caccavale

et al. 2023

IJMS

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Nitric oxide (NO) is a key signaling molecule in almost all organisms and is active in a variety of physiological and pathological processes. Our understanding of the peculiarities and functions of this simple gas has increased considerably by extending studies to non-mammal vertebrates and invertebrates. In this review, we report the nitric oxide synthase (Nos) genes so far characterized in chordates and provide an extensive, detailed, and comparative analysis of the function of NO in the aquatic chordates tunicates, cephalochordates, teleost fishes, and amphibians. This comprehensive set of data adds new elements to our understanding of Nos evolution, from the single gene commonly found in invertebrates to the three genes present in vertebrates.

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Norepinephrine depresses the nitric oxide production in the ascidian hemocytes

Cited by 11 publications

References 25 publications

Mutual modulation between norepinephrine and nitric oxide in haemocytes during the mollusc immune response

Mutual modulation between norepinephrine and nitric oxide in haemocytes during the mollusc immune response

Methylene blue therapy versus standard treatment for acute-phase septic shock: a pilot randomized controlled trial

Nitric Oxide Function and Nitric Oxide Synthase Evolution in Aquatic Chordates

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