Normalization of asparagus somatic embryogenesis using a maltose-containing medium

Kunitake, Hisato; Nakashima, Toshiki; Mori, Kinya; Tanaka, Masanobu

doi:10.1016/s0176-1617(97)80098-3

Cited by 25 publications

(9 citation statements)

References 20 publications

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“…However, no healthy plantlets were recovered after culture on the maltose-containing medium (data not shown). These results contradict those from other reports that demonstrated a promoter effect from maltose in a number of systems, e.g., asparagus somatic embryo development (Kunitake et al, 1997) and pollen embryogenesis in bell peppers (Dolcet-Sanjuan et al, 1997). Future studies should examine more closely the effect of maltose and other carbohydrates in somatic embryogenesis of different pepper cultivars.…”

Section: Effect Of Sugar Typecontrasting

confidence: 99%

Factors important for somatic embryogenesis in zygotic embryo explants ofCapsicum annuum L.

Bodhipadma

Leung

2002

J. Plant Biol.

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We used four cultivars of Capsicum annuum L. -. Sweet Banana, California Wonder, Yolo Wonder, and Ace --to reexamine the critical factors influencing somatic embryogenesis from zy~tic embryo explants, as reported in the literature. When we followed the protocol of Buyukalaca and Mavituna (1996), which had induced somatic embryogenesis from mature zygotic embryos of cv. Ace, only callus was formed without embryogenesis from our mature zygotic embryo explants. Using the procedures of Harini and Lakshmi Sita (1993) and Binzel et al. (1996), with some modifications, we were able to induce somatic embryogenesis in all four cultivars. Rates of conversion were significantly reduced, from 75% and 65% to 40% and 28% in 'Sweet Banana' and 'California Wonder', respectively, when the immature zygotic embryo explants were held on the induction medium for longer than two weeks. Likewise, somatic embryogenesis of 'Yolo Wonder' was not observed if the induction medium was supplemented with 10% glucose or fructose, or without 10% sucrose. For somatic embryo induction and eventual plantlet conversion in ~'olo Wonder', maltose could adequately replace sucrose. In all four cultivars, somatic embryos were initiated from immature zygotic explants on media with or without coconut water, under both light and dark conditions.

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Section: Effect Of Sugar Typecontrasting

confidence: 99%

Factors important for somatic embryogenesis in zygotic embryo explants ofCapsicum annuum L.

Bodhipadma

Leung

2002

J. Plant Biol.

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“…Karsai et al (1994) reported that maltose increased callus proliferation and plant formation in androgenesis of triticale and wheat. Maltose also increased regeneration of indica and japonica rice (Biswas and Zapata 1993;Jain et al 1997) and germination of asparagus embryos (Kunitake et al 1997).…”

Section: Discussionmentioning

confidence: 94%

The effect of genotype, media composition, pH and sugar concentrations on oat (Avena sativa L.) doubled haploid production through oat × maize crosses

et al. 2018

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Doubled haploid (DH) technology in oat has not reached the same stage as in other cereals leading to its application in plant breeding. The objective of this investigation was to increase the effectiveness of Avena sativa L. haploid embryo germination obtained by the distant crosses with maize. Developed embryos (obtained from 22 genotypes) were transferred on five germination media: MS (Murashige and Skoog, Physiol Plant 15:473-497, 1962) with 3% sucrose, pH 5.8 (control medium), and 190-2 supplemented with 6 and 9% maltose. The pH of 190-2 was adjusted to 5.5 and 6.0. Of all tested genotypes, 591 haploid embryos were obtained, almost half of them (279) germinated. The rate of haploid embryo germination induced on 190-2 was 6.92%, while in MS it was 3.25%. The sugar and its concentration significantly affected the germination of haploid embryos. The highest percentage of haploid embryo germination (9.11%) and DH lines production (1.64%) was achieved on 190-2 with 9% maltose and pH 6.0. All DH lines are incorporated to breeding programs for the development of new cultivars.

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“…These results might have been a consequence of the activity of ancymidol, which reduced the levels of endogenous gibberellin, and ABA, which does not inhibit GA biosynthesis but counteracts its activity in somatic embryogenesis. Li and Wolyn (1995) had obtained 150 embryos per 1 g of callus and a 38% conversion rate on media supplemented with ancymidol or ABA (no statistical differences), while Kunitake et al (1997) reported a 20-80% germination rate, depending on the source of carbohydrates, and Dasgupta et al (2007) -25 bipolar embryos per 1 g of tissue. According to most reports, many of the asparagus genotypes may be non-responsive to somatic embryogenesis.…”

Section: Indirect Embryogenesismentioning

confidence: 93%

Micropropagation of Asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis

Pindel

2017

Folia Horticulturae

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The present study has described a simple protocol for efficient plant regeneration of Asparagus densiflorus 'Sprengeri' and 'Myriocladus' using single-node spear explants, and indirect organogenesis via callogenesis induced on internode explants. The results showed that the genotypes 'Sprengeri' and 'Myriocladus' regenerated to complete plants via nodal cultures and callus tissue, but the plant regeneration response was higher in secondary explants on MS medium with NAA + kinetin (1+1 mg dm ). Primary explants of both cultivars showed high regenerative potential (via the callus stage) on MS medium with IAA+BAP. The cultivar Sprengeri also regenerated via somatic embryogenesis. Both kinds of 'Meyeri' explants have a morphogenetic potential for the formation of shoots, which, however, were not capable of rooting. This confirms that the explant, genotype and culture medium are determining factors in the in vitro plant regeneration system.

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Normalization of asparagus somatic embryogenesis using a maltose-containing medium

Cited by 25 publications

References 20 publications

Factors important for somatic embryogenesis in zygotic embryo explants ofCapsicum annuum L.

Factors important for somatic embryogenesis in zygotic embryo explants ofCapsicum annuum L.

The effect of genotype, media composition, pH and sugar concentrations on oat (Avena sativa L.) doubled haploid production through oat × maize crosses

Micropropagation of Asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis

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