Nosema sp. infecting a laboratory colony of the Alfalfa Weevil

Orthosomella lipae sp. n. (Microsporidia) a parasite of the weevil, Liophloeus lentus Germar, 1824 (Coleoptera: Curculionidae)

“…Most descriptions are incomplete and include only morphological features observed under the light microscope (Table 1). Seven of these species were described as belonging to the genus Nosema (Weiser, 1951;Maddox and Luckmann, 1966;Issi and Lipa, 1968;Drea et al, 1969;McLaughlin, 1969;Streett et al, 1975;Youssef, 1974), two were provisionally placed within the genera Canningia (Bruck and Solter, 2005;Bruck et al, 2008) and Unikaryon (Ś wią tek and Górkiewicz, 2006) and four were placed within the collective genus Microsporidium (Hesse, 1905;Malone, 1985;Ghosh, 1990;Lewis et al, 2003). Among microsporidian parasites of weevils only Microsporidium itiiti infecting Argentine stem weevil Listronotus bonariensis, have been studied ultrastucturally (Malone, 1985).…”

Long‐Term Storage of Infective Microsporidian Spores in Liquid Nitrogen

“…Nosema sp. was obtained in 1968 from an infected laboratory colony [2]. Samples of the microsporidium were placed in liquid nitrogen as partially macerated infected larvae suspended in one of three media: 100% water, 50% glycerol, or 100°/o glycerol.…”

“…This study on the use of liquid nitrogen for storing microsporidian spores addresses three specific questions: (1) Can spores of most microsporidia survive storage in liquid nitrogen? (2) How long can spores survive in liquid nitrogen? and (3) Do cryoprotectants improve the survival of spores in liquid nitrogen?…”