NOSH–aspirin (NBS-1120), a novel nitric oxide- and hydrogen sulfide-releasing hybrid is a potent inhibitor of colon cancer cell growth in vitro and in a xenograft mouse model

Chattopadhyay, Matangini; Kodela, Ravinder; Olson, Kenneth R.; Kashfi, Khosrow

doi:10.1016/j.bbrc.2012.02.051

Cited by 116 publications

(107 citation statements)

References 27 publications

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“…Our research (31) and other studies (24)(25)(26)(27)(28)(29)(30) suggest that H 2 S is beneficial for cancer cells growth, proliferation, migration and invasion. On the contrary, some other researchers found that H 2 S exerted potential anticancer efficiency in SGC-7901 gastric cancer cells (40), oral cancer cell lines (41), colon cancer cells (42), and in several different human cancer cell lines (HeLa, HCT-116, Hep G2, HL-60, MCF-7, MV4-11 and U2OS) (43). As the above is contradictory further research must be carried out to clarify these issues.…”

Section: Discussionmentioning

confidence: 93%

Exogenous hydrogen sulfide promotes C6 glioma cell growth through activation of the p38 MAPK/ERK1/2-COX-2 pathways

et al. 2015

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Abstract. Hydrogen sulfide (H 2 S) participates in multifarious physiological and pathophysiologic progresses of cancer both in vitro and in vivo.We have previously demonstrated that exogenous H 2 S promoted liver cancer cells proliferation/anti-apoptosis/angiogenesis/migration effects via amplifying the activation of NF-κB pathway. However, the effects of H 2 S on cancer cell proliferation and apoptosis are controversial and remain unclear in C6 glioma cells. The present study investigated the effects of exogenous H 2 S on cancer cells growth via activating p38 MAPK/ERK1/2-COX-2 pathways in C6 glioma cells. C6 glioma cells were treated with 400 µmol/l NaHS (a donor of H 2 S) for 24 h. The expression levels of phosphorylated (p)-p38 MAPK, total (t)-p38 MAPK, p-ERK1/2, t-ERK1/2, cyclooxygenase-2 (COX-2) and caspase-3 were measured by western blotting assay. Cell viability was detected by Cell Counting . Apoptotic cells were observed by Hoechst 33258 staining assay. Cell proliferation was directly detected under fully automatic inverted microscope. Exposure of C6 glioma cells to NaHS resulted in cell proliferation, as evidenced by an increase in cell viability. In addition, NaHS treatment reduced apoptosis, as indicated by the decreased apoptotic percentage and the cleaved caspase-3 expression. Importantly, exposure of the cells to NaHS increased the expression levels of p-p38 MAPK, p-ERK1/2 and COX-2. Notably, co-treatment of C6 glioma cells with 400 µmol/l NaHS and AOAA (an inhibitor of CBS) largely suppressed the above NaHS-induced effects. Combined treatment with NaHS and SB203580 (an inhibitor of p38 MAPK) or PD-98059 (an inhibitor of ERK1/2) resulted in the synergistic reduction of COX-2 expression and increase of caspase-3 expression, a decreased number of apoptotic cells, along with decreased cell viability. Combined treatment with NS-398 (an inhibitor of COX-2) and NaHS also resulted in the synergistic increase of caspase-3, a decreased in the number of apoptotic cells and the decrease in cell viability. The findings of the present study provide novel evidence that p38 MAPK/ERK1/2-COX-2 pathways are involved in NaHS-induced cancer cell proliferation and anti-apoptosis in C6 glioma cells.

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Section: Discussionmentioning

confidence: 93%

Exogenous hydrogen sulfide promotes C6 glioma cell growth through activation of the p38 MAPK/ERK1/2-COX-2 pathways

et al. 2015

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“…Similarly, H 2 S-releasing aspirin, a novel and safer derivative of aspirin, has shown promise as an anticancer agent against hormone-independent estrogen receptor-negative breast cancers by inhibiting cell proliferation, inducing apoptosis, and decreasing NF-B levels (79). Moreover, NOSH-aspirin, a NOand H 2 S-releasing agent, has been found to inhibit colon cancer growth by inhibiting cell proliferation, inducing apoptosis, and causing G 0 /G 1 cell cycle block (80). Overall, these results indirectly demonstrate that H 2 S has strong anticancer potential that merits further evaluation.…”

Section: H 2 S: a Novel Therapeutic Agent In Age-associated Diseases?mentioning

confidence: 99%

Hydrogen Sulfide, the Next Potent Preventive and Therapeutic Agent in Aging and Age-Associated Diseases

Zhang

Tang

Zhong

et al. 2013

Molecular and Cellular Biology

156

109

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Hydrogen sulfide (H 2 S) is the third endogenous signaling gasotransmitter, following nitric oxide and carbon monoxide. It is physiologically generated by cystathionine-␥-lyase, cystathionine-␤-synthase, and 3-mercaptopyruvate sulfurtransferase. H 2 S has been gaining increasing attention as an important endogenous signaling molecule because of its significant effects on the cardiovascular and nervous systems. Substantial evidence shows that H 2 S is involved in aging by inhibiting free-radical reactions, activating SIRT1, and probably interacting with the age-related gene Klotho. Moreover, H 2 S has been shown to have therapeutic potential in age-associated diseases. This article provides an overview of the physiological functions and effects of H 2 S in aging and age-associated diseases, and proposes the potential health and therapeutic benefits of H 2 S.

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“…28 Several H 2 S-releasing drugs (or prodrugs) have been developed to conjugate the beneficial effects of H 2 S with those of other pharmaceuticals. 29 SH-aspirin (SH-ASA) is one of these H 2 S-releasing nonsteroidal anti-inflammatory drugs (HS-NSAIDs), in which the H 2 S-releasing moiety is covalently attached to aspirin ( Figure 1B). Its H 2 S-releasing ability was realized through the hydrolysis of the ester bond in SH-ASA.…”

mentioning

confidence: 99%

“…HS-NSAIDs have been reported recently to have potential cancer-controlling bioactivity. [29][30][31][32] Their difficulties in water solubility also limit further development. Nanotechnology provides optimizing strategies when solving the issue of poor water solubility of hydrophobic drugs.…”

mentioning

confidence: 99%

Codelivery of SH-aspirin and curcumin by mPEG-PLGA nanoparticles enhanced antitumor activity by inducing mitochondrial apoptosis

Zhou¹,

Duan²,

Zeng³

et al. 2015

IJN

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Natural product curcumin (Cur) and H 2 S-releasing prodrug SH-aspirin (SH-ASA) are potential anticancer agents with diverse mechanisms, but their clinical application prospects are restricted by hydrophobicity and limited efficiency. In this work, we coencapsulated SH-ASA and Cur into methoxy poly(ethylene glycol)-poly (lactide-coglycolide) (mPEG-PLGA) nanoparticles through a modified oil-in-water single-emulsion solvent evaporation process. The prepared SH-ASA/Cur-coloaded mPEG-PLGA nanoparticles had a mean particle size of 122.3±6.8 nm and were monodispersed (polydispersity index =0.179±0.016) in water, with high drug-loading capacity and stability. Intriguingly, by treating with SH-ASA/Cur-coloaded mPEG-PLGA nanoparticles, obvious synergistic anticancer effects on ES-2 and SKOV3 human ovarian carcinoma cells were observed in vitro, and activation of the mitochondrial apoptosis pathway was indicated. Our results demonstrated that SH-ASA/Cur-coloaded mPEG-PLGA nanoparticles could have potential clinical advantages for the treatment of ovarian cancer.

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NOSH–aspirin (NBS-1120), a novel nitric oxide- and hydrogen sulfide-releasing hybrid is a potent inhibitor of colon cancer cell growth in vitro and in a xenograft mouse model

Cited by 116 publications

References 27 publications

Exogenous hydrogen sulfide promotes C6 glioma cell growth through activation of the p38 MAPK/ERK1/2-COX-2 pathways

Exogenous hydrogen sulfide promotes C6 glioma cell growth through activation of the p38 MAPK/ERK1/2-COX-2 pathways

Hydrogen Sulfide, the Next Potent Preventive and Therapeutic Agent in Aging and Age-Associated Diseases

Codelivery of SH-aspirin and curcumin by mPEG-PLGA nanoparticles enhanced antitumor activity by inducing mitochondrial apoptosis

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