Nosocomial, Multidrug-Resistant<i>Klebsiella pneumoniae</i>Strains Isolated from Mexico City Produce Robust Biofilms on Abiotic Surfaces but Not on Human Lung Cells

Ostria-Hernández, Martha Lorena; Rosa, Karla Cecilia Juárez-de la; Arzate-Barbosa, Patricia; Lara-Hernández, Antonino; Sakai, Fuminori; Ibarra, J. Antonio; Castro-Escarpulli, Graciela; Vidal, Jorge E.

doi:10.1089/mdr.2017.0073

Cited by 23 publications

(21 citation statements)

References 41 publications

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“…Other studies showed similar or slightly higher proportion of Enterobacter strains that can form biofilms 28,29 . The high rate of biofilm-producing K. pneumoniae and the variability among E. coli isolates found in this study are in concordance with other studies evaluating MDR strains 14,30,31 .…”

Section: Discussionsupporting

confidence: 93%

Biofilm formation by multidrug resistant Enterobacteriaceae strains isolated from solid organ transplant recipients

Ramos-Vivas

Chapartegui-González

Fernández-Martínez

et al. 2019

Sci Rep

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Solid organ transplant (SOT) recipients are especially at risk of developing infections by multidrug resistant bacteria (MDR). In this study, the biofilm-forming capability of 209 MDR strains ( Escherichia coli n = 106, Klebsiella pneumoniae n = 78, and Enterobacter spp. n = 25) isolated from rectal swabs in the first 48 hours before or after kidney (93 patients), liver (60 patients) or kidney/pancreas transplants (5 patients) were evaluated by using a microplate assay. Thirty-nine strains were isolated before transplant and 170 strains were isolated post-transplant. Overall, 16% of E. coli strains, 73% of K. pneumoniae strains and 4% Enterobacter strains showed moderate or strong biofilm production. Nine strains isolated from infection sites after transplantation were responsible of infections in the first month. Of these, 4 K. pneumoniae , 1 E. coli and 1 Enterobacter spp. strains isolated pre-transplant or post-transplant as colonizers caused infections in the post-transplant period. Our results suggest that in vitro biofilm formation could be an important factor for adhesion to intestine and colonization in MDR K. pneumoniae strains in SOT recipients, but this factor appears to be less important for MDR E. coli and Enterobacter spp.

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Section: Discussionsupporting

confidence: 93%

Biofilm formation by multidrug resistant Enterobacteriaceae strains isolated from solid organ transplant recipients

Ramos-Vivas

Chapartegui-González

Fernández-Martínez

et al. 2019

Sci Rep

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“…Recently, several studies report the association of antibiotic resistance with biofilm formation in clinical isolates of Klebsiella spp. [11][12][13][14]. Moreover, the role of fimbriae in adhesion and biofilm formation by Klebsiella is also well documented [15,16].…”

Section: Introductionmentioning

confidence: 96%

High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae

2019

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Klebsiella pneumoniae (Kp), is a frequent cause of hospital and community-acquired infections and WHO had declared it as a "priority pathogen". Biofilm is a major virulence factor of Kp and yet the mechanism of strong biofilm formation in Kp is unclear. A key objective of the present study is to investigate the differences between strong and weak biofilms formed by clinical isolates of Kp on various catheters and in different media conditions and to identify constituents contributing to strong biofilm formation. Quantification of matrix components (extracellular DNA (eDNA), protein, exopolysaccharides (EPS), and bacterial cells), confocal laser scanning microscopy (CLSM), field emission gun scanning electron microscopy (FEG-SEM) and flow-cytometry analysis were performed to compare strong and weak biofilm matrix. Our results suggest increased biofilm formation on latex catheters compared to silicone and silicone-coated latex catheters. Higher amounts of eDNA, protein, EPS, and dead cells were observed in the strong biofilm of Kp. High adhesion capacity and cell death seem to play a major role in formation of strong Kp biofilms. The enhanced eDNA, EPS, and protein in the biofilm matrix appear as a consequence of increased cell death.

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“…Antibiotic resistance was determined in clinical isolated strains using Vitek2 ® , so classification according to criteria described in this work, could be applied, obtaining a higher MDR strains percentage (16) reported that 80% of a Mexico City hospital isolated strains were MDR, nevertheless, no PDR strain was obtained, whereas in this study, one PDR strain was found. This allows us to visualize the increasing resistance of K. pneumoniae due to indiscriminate use of antibiotics for treatment of infection caused by this bacterium.…”

Section: Discussionmentioning

confidence: 84%

First report of New Clonal groups ST706 and ST1088 from MDR Klebsiella pneumoniae Mexican strains

Córdova-Espinoza

Arana

Giono-Cerezo

et al. 2019

Preprint

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Multidrug-resistant Klebsiella pneumoniae Mexican strains were characterized for the identification of endemic and pandemic clonal groups. The aims of this study were to know population structure and to identify endemic clonal groups inside K. pneumoniae Mexican strains isolated from clinical sources. We studied 93 isolated strains from three third level hospitals and one family clinic from Mexico City. Identification of the strains was done by conventional microbiological methods and an automated system (Vitek2®). The multidrug-resistant phenotype was confirmed following CLSI recommendations, and the strains were classified as MDR, XDR and PDR. Molecular characterization was done by Multilocus Sequence Typing scheme (rpoB, gapA, mdh, pgi, phoE, infB, y tonB). All strains were isolated from hospitalized patients, the most frequent sources were urine and blood cultures. Population structure of K. pneumoniae was clonal, 30 ST were identified, six of them are commonly found. The Clonal complex ST25, ST36, S5392, ST405 and ST551 were isolated from clinical sources, ST1088 was isolated from surfaces of hospital environment.

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Nosocomial, Multidrug-ResistantKlebsiella pneumoniaeStrains Isolated from Mexico City Produce Robust Biofilms on Abiotic Surfaces but Not on Human Lung Cells

Abstract: Kpn strains isolated from nosocomial infections in Mexico City are MDR, although the majority are still susceptible to carbapenems and form more robust biofilms on polystyrene in comparison to those formed on human cells.

Cited by 23 publications

References 41 publications

Biofilm formation by multidrug resistant Enterobacteriaceae strains isolated from solid organ transplant recipients

Biofilm formation by multidrug resistant Enterobacteriaceae strains isolated from solid organ transplant recipients

High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae

First report of New Clonal groups ST706 and ST1088 from MDR Klebsiella pneumoniae Mexican strains

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