Nosocomial outbreak of extended-spectrum β-lactamase-producing Enterobacter ludwigii co-harbouring CTX-M-8, SHV-12 and TEM-15 in a neonatal intensive care unit in Venezuela

Flores-Carrero, Ana; Labrador, Indira; Paniz‐Mondolfi, Alberto; Peaper, David R.; Towle, Dana; Araque, Marı́a

doi:10.1016/j.jgar.2016.08.006

Cited by 16 publications

(8 citation statements)

References 21 publications

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“…DNA was extracted using the X-trem preparation kit (Biotech, Granada, Spain). bla TEM , bla SHV and group bla CTX-M genes were detected by PCR based on previously described primers and protocols [ 17 ]. All amplification products were purified (AccuPrep1 PCR Kit; Bioneer, Daejeon, South Korea) and nucleotide sequencing was performed using a 3730XL Genetic Analyzer (Applied Biosystems, Carlsbad, CA).…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing <italic>Escherichia coli</italic> in Healthy Children from a Rural Andean Village in Venezuela

Araque¹,

Labrador²

2018

Osong Public Health Res Perspect

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ObjectivesAntimicrobial resistant extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-PE) have been shown to be present in healthy communities. This study examined healthy children from the rural Andean village of Llano del Hato, Mérida, Venezuela, who have had little or no antibiotic exposure to determine the prevalence of fecal carriage of ESBL-producing Escherichia coli (ESBL-EC).MethodsA total of 78 fecal samples were collected in healthy children aged from 1 to 5 years. ESBL-EC were selected in MacConkey agar plates with cefotaxime and further confirmed by the VITEK 2 system. ESBL were phenotypically detected and presence of bla genes and their variants were confirmed by molecular assays. Determination of phylogenetic groups was performed by PCR amplification. Risk factors associated with fecal carriage of ESBL-EC-positive isolates were analyzed using standard statistical methods.ResultsOf the 78 children studied, 27 (34.6%) carried ESBL-EC. All strains harbored the blaCTX-M-15 allele. Of these, 8 were co-producers of blaTEM-1, blaTEM-5, blaSHV-5 or blaSHV-12. Co-resistance to aminoglycosides and/or fluoroquinolones was observed in 9 strains. 51.9% of ESBL-EC isolates were classified within phylogroup A. A significant, positive correlation was found between age (≥2.5 – ≤5 years), food consumption patterns and ESBL-EC fecal carriage.ConclusionThis is the first study describing the high prevalence of fecal carriage of ESBL-EC expressing CTX-M-15- among very young, healthy children from a rural Andean village in Venezuela with scarce antibiotic exposure, underlining the importance of this population as a reservoir.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing <italic>Escherichia coli</italic> in Healthy Children from a Rural Andean Village in Venezuela

Araque¹,

Labrador²

2018

Osong Public Health Res Perspect

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“…Some strains exhibit resistance to third generation cephalosporine, but not to cotrimoxazole, gentamicin, imipenem, and ciprofloxacin [3]. Furthermore, genes mediating carbapenem resistance [8] and genes mediating extended spectrum β-lactamases (ESBLs) [9] were described in E. ludwigii. In our case, the agent was susceptible to meropenem, a carbapenem, and to ceftazidime, a third generation cephalosporine.…”

Section: Discussionmentioning

confidence: 99%

“…Only a few clinical cases were described to this date. Enterobacter ludwigii was isolated from orthopedic implants infections [11], a surgical wound [8], BSIs in neonates [9], and CABSI in hemodialysis patients [12,13]. Additionally, 18 E. ludwigii isolates were reported in a single study mainly from blood cultures, genitourinary and gastrointestinal tract [14].…”

Section: Discussionmentioning

confidence: 99%

Bloodstream infection due to Enterobacter ludwigii, correlating with massive aggregation on the surface of a central venous catheter

2020

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We report a case of catheter associated bloodstream infection due to Enterobacter ludwigii with a massive aggregation on the outside surface of a central venous catheter (CVC). The 57 years old patient with a history of spondylodiscitis and Staphylococcus aureus-associated endocarditis was admitted to the intensive care unit for acute cerebral infarction. The patient developed signs of infections and the CVC was removed 11 days after placement. The infectious agent was identified by standard diagnostics to the genus level as belonging to the Enterobacter cloacae complex, and additional molecular testing determined the species as E. ludwigii. The catheter was selected for a study aiming to identify the influence of blood components on the formation of central venous catheter-associated biofilms. In this course a massive biofilm was recognized and is presented here.

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“…In the present research, we proved that E. ludwigii directly promoted DCs' tolerance function to enhance Treg differentiation. E. ludwigii is a motile rod-shaped gram-negative bacterium, and some E. ludwigii isolates with antibiotic resistance genes have been reported to be associated with surgical site infections or nosocomial bloodstream infections [43,44]. Therefore, E. ludwigii strains should be carefully considered for direct application.…”

Section: Discussionmentioning

confidence: 99%

Enterobacter Ludwigii Protects Experimental Colitis Through CD103+DC and Treg Cells

Li¹,

Sun²,

Kobayashi³

et al. 2020

Preprint

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Background: Intestinal bacteria are closely related with inflammatory bowel disease (IBD), and regulatory cell-mediated immune tolerance is important to inhibit IBD. Commensal intestinal bacteria play key roles in regulating immune tolerance cell, however, bacterial strains directly involved in this regulation remain to be identified.Results: In the present study, metronidazole, among nine antibiotics, was found to have the best effect on protecting mice against DSS-induced colitis. Enterobacter ludwigi, abundant in mouse feces after metronidazole treatment, was identified to decrease mice susceptibility to DSS-induced colitis with or without the presence of complex intestinal bacteria. E. ludwigii gavage increased CD103+DCs and Foxp3+Treg cells in intestinal microenvironment, and effects of E. ludwigii on diminishing colitis were lost in DC or Treg depletion mice. CD103+DCs isolated from E. ludwigii-treated mice showed enhanced ability to promote the Treg differentiation from naive T cells. DCs, directly stimulated by live E. ludwigii strain or its culture supernatant, had increased immune tolerance ability for Treg differentiation in vitro. Conclusions: Overall, our findings identify a facultative anaerobe bacterial strain E. ludwigii, which directly enhances CD103+DC and Treg-mediated immune tolerance, resulting in protecting mice against DSS-induced colitis.

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Nosocomial outbreak of extended-spectrum β-lactamase-producing Enterobacter ludwigii co-harbouring CTX-M-8, SHV-12 and TEM-15 in a neonatal intensive care unit in Venezuela

Cited by 16 publications

References 21 publications

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing <italic>Escherichia coli</italic> in Healthy Children from a Rural Andean Village in Venezuela

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing <italic>Escherichia coli</italic> in Healthy Children from a Rural Andean Village in Venezuela

Bloodstream infection due to Enterobacter ludwigii, correlating with massive aggregation on the surface of a central venous catheter

Enterobacter Ludwigii Protects Experimental Colitis Through CD103+DC and Treg Cells

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Nosocomial outbreak of extended-spectrum β-lactamase-producing Enterobacter ludwigii co-harbouring CTX-M-8, SHV-12 and TEM-15 in a neonatal intensive care unit in Venezuela

Cited by 16 publications

References 21 publications

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing &lt;italic&gt;Escherichia coli&lt;/italic&gt; in Healthy Children from a Rural Andean Village in Venezuela

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing &lt;italic&gt;Escherichia coli&lt;/italic&gt; in Healthy Children from a Rural Andean Village in Venezuela

Bloodstream infection due to Enterobacter ludwigii, correlating with massive aggregation on the surface of a central venous catheter

Enterobacter Ludwigii Protects Experimental Colitis Through CD103+DC and Treg Cells

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Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing <italic>Escherichia coli</italic> in Healthy Children from a Rural Andean Village in Venezuela

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing <italic>Escherichia coli</italic> in Healthy Children from a Rural Andean Village in Venezuela