Subject librarianship (SL) has long been practiced in academic libraries. But, with the transformation brought about by socio-cultural and technological factors, the roles have evolved. This article discussed the traditional role of Kazakh subject librarians, their current practices, and personal strategies in delivering services including information literacy in academic libraries. In Kazakhstan, subject librarians provide information literacy sessions and workshops dedicated for specific classes. A quantitative research method was adopted for this study using an online questionnaire for data gathering. It utilized purposive sampling to identify the competencies and performing tasks of subject librarians. It also analyzed how subject specialists promote their programs and relationship building strategies and investigates the differences and similarities with the available Western interpretations of subject librarianship. A total of 57 Kazakh university libraries and 80 librarians responded to the survey questions. Results of the research provides guidance for the implementation of subject librarianship practices of academic libraries in the region. To better serve their communities, subject librarians shall develop an excellent relationship, shared commitment, offer quality assistance, open communication, and always instils professionalism. Ultimately, the study reflects on the uniqueness of subject librarianship in Kazakhstan as well as its similarity in the practice of subject librarianship globally.