2017
DOI: 10.1159/000471945
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Notch Signaling Augments BMP9-Induced Bone Formation by Promoting the Osteogenesis-Angiogenesis Coupling Process in Mesenchymal Stem Cells (MSCs)

Abstract: Background/Aims: Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into several lineages including bone. Successful bone formation requires osteogenesis and angiogenesis coupling of MSCs. Here, we investigate if simultaneous activation of BMP9 and Notch signaling yields effective osteogenesis-angiogenesis coupling in MSCs. Methods: Recently-characterized immortalized mouse adipose-derived progenitors (iMADs) were used as MSC source. Transgenes BMP9, NICD and dnNotch1 were express… Show more

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Cited by 1,993 publications
(90 citation statements)
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“…Subcutaneous stem cell implantation was performed as previously described [26, 27]. Briefly, BMSCs were infected with different lentiviruses.…”
Section: Methodsmentioning
confidence: 99%
“…Subcutaneous stem cell implantation was performed as previously described [26, 27]. Briefly, BMSCs were infected with different lentiviruses.…”
Section: Methodsmentioning
confidence: 99%
“…The GLuc reporters for miR-223 and let-7a-1 were constructed on the base of our recently characterized reporter vector pNRGLuc, which express Gaussia luciferase (GLuc) with a multiple cloning linker at the 3′-end of GLuc coding region [54,55]. As human ARRB1 and LIN28B are well established targets for miR-223 and let-7a-1, which contain miR-223 and let-7a-1-binding sites at their 3′-UTRs, respectively, we PCR amplified the 3′-untranslated sequences of human ARRB1 (or BUTR) and LIN28B, and subcolned them into pNRGLuc vector, resulting in pNRGLuc-BUTR (or GLuc-BUTR) and pNRGLuc-LIN28B (or GLuc-LIN28B), respectively.…”
Section: Construction Of Mir-223 and Let-7a-1 Gaussia Luciferase (Glumentioning
confidence: 99%
“…The Notch signalling pathway is involved in cell proliferation, differentiation and apoptosis 69 . In mammals, the Notch signalling pathway contains four Notch receptors, Notch1‐4 and five ligands, Delta‐like (DLL) 1, DLL3, DLL4, Jagged1 and Jagged2 70 . Notch receptors are transmembrane proteins composed of a Notch intracellular domain, transmembrane domain and Notch extracellular domain 71,72 .…”
Section: Factors Involved In Bone Regeneration By Regulating Type H Vmentioning
confidence: 99%