Notch Signaling Response to Heavy Compression Force Induces Orthodontic Root Resorption via RANKL and IL-6 from Cementoblasts

Iwane, Takehiro; Kikuta, Jun

doi:10.5466/ijoms.18.287

Cited by 4 publications

(7 citation statements)

References 23 publications

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“…Diercke et al [ 103 ] demonstrated that IL-1β and compressive forces lead to a significant induction of RANKL expression in cementoblasts. Minato et al [ 110 ] and Iwane et al [ 111 ] reported that the exposure of cementoblasts to excessive orthodontic forces also produces a large amount of RANKL and IL-6, which induce the process of OIIRR. Therefore, the occurrence of OIIRR is caused by both PDL cells and cementoblasts.…”

Section: Inflammation Of Orthodontically Induced Inflammatory Rootmentioning

confidence: 99%

Is Inflammation a Friend or Foe for Orthodontic Treatment?: Inflammation in Orthodontically Induced Inflammatory Root Resorption and Accelerating Tooth Movement

Yamaguchi¹,

Fukasawa²

2021

IJMS

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The aim of this paper is to provide a review on the role of inflammation in orthodontically induced inflammatory root resorption (OIIRR) and accelerating orthodontic tooth movement (AOTM) in orthodontic treatment. Orthodontic tooth movement (OTM) is stimulated by remodeling of the periodontal ligament (PDL) and alveolar bone. These remodeling activities and tooth displacement are involved in the occurrence of an inflammatory process in the periodontium, in response to orthodontic forces. Inflammatory mediators such as prostaglandins (PGs), interleukins (Ils; IL-1, -6, -17), the tumor necrosis factor (TNF)-α superfamily, and receptor activator of nuclear factor (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) are increased in the PDL during OTM. OIIRR is one of the accidental symptoms, and inflammatory mediators have been detected in resorbed roots, PDL, and alveolar bone exposed to heavy orthodontic force. Therefore, these inflammatory mediators are involved with the occurrence of OIIRR during orthodontic tooth movement. On the contrary, regional accelerating phenomenon (RAP) occurs after fractures and surgery such as osteotomies or bone grafting, and bone healing is accelerated by increasing osteoclasts and osteoblasts. Recently, tooth movement after surgical procedures such as corticotomy, corticision, piezocision, and micro-osteoperforation might be accelerated by RAP, which increases the bone metabolism. Therefore, inflammation may be involved in accelerated OTM (AOTM). The knowledge of inflammation during orthodontic treatment could be used in preventing OIIRR and AOTM.

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Section: Inflammation Of Orthodontically Induced Inflammatory Rootmentioning

confidence: 99%

Is Inflammation a Friend or Foe for Orthodontic Treatment?: Inflammation in Orthodontically Induced Inflammatory Root Resorption and Accelerating Tooth Movement

Yamaguchi¹,

Fukasawa²

2021

IJMS

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“…When one of these PPi factors is removed from the equation, apposition cannot be fully regulated and cementoblasts attempt to compensate by increasing the expression of its counterpart PPi regulator [ 32 , 33 , 34 , 35 ]. With directly controlling cementum mineral apposition, these studies suggest that PPi influences ECM (extracellular matrix) protein composition [ 36 , 37 , 38 , 39 ]. In the circumstances of rapid cementogenesis, cementoblasts are increasing the expression of OPN (osteopontin) and DMP1 (dentin matrix phosphoprotein).…”

Section: Resultsmentioning

confidence: 99%

Periodontal Disease Monitoring by Raman Spectroscopy of Phosphates: New Insights into Pyrophosphate Activity

Gatin,

Iordache,

Gatin

et al. 2023

Diagnostics

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(1) Background: The intent of this survey was to investigate the quality of the alveolar bone by revealing the different phases for calcified tissues independent of the medical history of the patient in relation to periodontal disease by means of Raman spectroscopy and then to correlate the results by suggesting a possible mechanism for the medical impairment; (2) Methods: The investigation was mainly based on Raman spectroscopy that was performed in vivo during surgery for the selected group of patients. The targeted peaks for the Raman spectra were according to the reference compounds (e.g., calcium phosphates, other phosphates); (3) Results: The variation in the intensity of the spectrum correlated to the specific bone constituents’ concentrations highlights the bone quality, while some compounds (such as pyrophosphate, PPi) are strongly related to the patient’s medical status, and they provide information regarding a physiological process that occurred in the calcified tissues. Moreover, bone sample fluorescence is related to the collagen (Col) content, enabling a complete evaluation of bone quality, revealing the importance of collagen matrix acting as a load-bearing element for Calcium phosphate (CaP) deposition during the complex bone mineralization process; (4) Conclusions: We highlight that Raman spectroscopy can be considered a viable investigative method for in vivo and rapid bone quality valuation through oral health monitoring.

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“…Second-to fifth-passage human PDL cells were seeded in duplicate into a Petri dish (100-mm) and cultured to confluence prior to further experimental stimulation in α-MEM containing 10% fetal bovine serum and 1% antibiotics (diluted from a stock solution containing 10,000 U/mL of penicillin and 10,000 U/mL of streptomycin; Biochrom AG, Berlin, Germany) at 37 • C in an atmosphere of 100% humidity, 95% air, and 5% CO 2 [22]. The culture medium was changed to 1% FBS α-MEM with penicillin and streptomycin, and a cover glass and weights were placed-for the optimal orthodontic force group (1.0 g/cm 2 ) and the heavy orthodontic force group (4.0 g/cm 2 )-on the top of the PDLF and HGF cell layers for 2, 4, 6, 8, 12, and 24 h cultures [23,24]. A cover glass (0.052 g/cm 2 ) was only placed for the control of the weight, and a combination of no glass and no weight was also used as a negative control.…”

Section: Cell Culture and Application Of Compression Forcementioning

confidence: 99%

“…To clarify the effects of compressive force on the expression of Shh, RANKL, and IL-6 in HGF and PDLF, an optimal orthodontic force (1.0 g/cm 2 ) and a strong orthodontic force (4.0 g/cm 2 ) were placed on the fibrosed layers of HGF and the PDLF cells and incubated for 2, 4, 6, 8, 12, and 24 h. The heavy force may repress cell viability and increase the mortality rate of hPDL [24]. First, we observed whether these compression forces damage the cells.…”

Section: Effects Of Orthodontic Force On the Cell Activities Adherenc...mentioning

confidence: 99%

Effects of Improper Mechanical Force on the Production of Sonic Hedgehog, RANKL, and IL-6 in Human Periodontal Ligament Cells In Vitro

Yamashita,

Negishi,

Kikuta

et al. 2024

Dentistry Journal

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Improper mechanical stress may induce side effects during orthodontic treatment. If the roots and alveolar bones are extensively resorbed following excess mechanical stress, unplanned tooth mobility and inflammation can occur. Although multiple factors are believed to contribute to the development of side effects, the cause is still unknown. Sonic hedgehog (Shh), one of the hedgehog signals significantly associated with cell growth and cancer development, promotes osteoclast formation in the jawbone. Shh may be associated with root and bone resorptions during orthodontic treatment. In this study, we investigated the relationships between Shh, RANKL, and IL-6 in human periodontal ligament (hPDL) cells exposed to improper mechanical force. Weights were placed on hPDL cells and human gingival fibroblasts (HGFs) for an optimal orthodontic force group (1.0 g/cm2) and a heavy orthodontic force group (4.0 g/cm2). A group with no orthodontic force was used as a control group. Real-time PCR, SDS-PAGE, and Western blotting were performed to examine the effects of orthodontic forces on the expression of Shh, RANKL, and IL-6 at 2, 4, 6, 8, 12, and 24 h after the addition of pressure. The protein expression of Shh was not clearly induced by orthodontic forces of 1.0 and 4.0 g/cm2 compared with the control in HGFs and hPDL cells. In contrast, RANKL and IL-6 gene and protein expression was significantly induced by 1.0 and 4.0 g/cm2 in hPDL cells for forces lasting 6~24 h. However, neither protein was expressed in HGFs. RANKL and IL-6 expressions in response to orthodontic forces and in the control were clearly inhibited by Shh inhibitor RU-SKI 43. Shh did not directly link to RANKL and IL-6 for root and bone resorptions by orthodontic force but was associated with cell activities to be finally guided by the production of cytokines in hPDL cells.

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Notch Signaling Response to Heavy Compression Force Induces Orthodontic Root Resorption via RANKL and IL-6 from Cementoblasts

Cited by 4 publications

References 23 publications

Is Inflammation a Friend or Foe for Orthodontic Treatment?: Inflammation in Orthodontically Induced Inflammatory Root Resorption and Accelerating Tooth Movement

Is Inflammation a Friend or Foe for Orthodontic Treatment?: Inflammation in Orthodontically Induced Inflammatory Root Resorption and Accelerating Tooth Movement

Periodontal Disease Monitoring by Raman Spectroscopy of Phosphates: New Insights into Pyrophosphate Activity

Effects of Improper Mechanical Force on the Production of Sonic Hedgehog, RANKL, and IL-6 in Human Periodontal Ligament Cells In Vitro

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