Note: Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species

Brett, Paul J.; DeShazer, David; Woods, Donald E.

doi:10.1099/00207713-48-1-317

Cited by 408 publications

(343 citation statements)

References 6 publications

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“…Hotspotting of transposon insertion events can occur in areas of a bacterial genome that differ in their GC base ratio when compared with the whole genome [44,45]. B. thailandensis, a close but non-capsulate relative of B. pseudomallei [46], has recently been shown to possess a truncated capsule polysaccharide operon [24] with truncation close to the insertion site of the transposon in B. pseudomallei 1E10. This lack of a capsule may be one reason that B. thailandensis strains are avirulent in mouse models.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis

Atkins

Prior

Mack

et al. 2002

Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 99%

Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis

Atkins

Prior

Mack

et al. 2002

Journal of Medical Microbiology

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“…There is evidence that B. thailandensis (Ara þ strains) [5] are avirulent whereas Ara À strains constitute melioidosis-causing 'true' B. pseudomallei [6]. It is possible to discriminate between the two biotypes by molecular approaches such as fliC variation [7,8] or multiplex PCR assays [9].…”

Section: Introductionmentioning

confidence: 99%

Distribution of type III secretion gene clusters in Burkholderia pseudomallei, B. thailandensis and B. mallei

Rainbow

Winstanley

2002

Journal of Medical Microbiology

109

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Burkholderia pseudomallei, the causative agent of melioidosis, carries a cluster of genes closely related in organisation to the type III secretion (TTS) system gene clusters of the plant pathogens Ralstonia solanacearum and Xanthomonas spp. The TTS gene cluster (TTS1) is present only in B. pseudomallei and not in avirulent B. thailandensis. Adjacent to the gene cluster encoding putative secreton structural proteins lie a number of open reading frames (ORFs) encoding putative proteins with little or no homology to known proteins, with the exception of one predicted protein with homology to Pseudomonas syringae HrpK. In both R. solanacearum and Xanthomonas spp., genes in this location encode secreted effector proteins. RT-PCR analysis indicated that TTS genes, including two of these ORFs, are expressed in broth at 378C. Analysis of genome sequence data identified a second cluster of TTS genes (TTS2) present in both B. pseudomallei and B. mallei (99% identity). However, B. mallei appears to lack the TTS1 gene cluster. PCR assays indicated that TTS2 was also present in B. thailandensis. TTS1 and TTS2 are similar in gene organisation, but nucleotide sequences are sufficiently divergent to suggest that the two TTS systems may have different roles.

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“…The genus of Burkholderia covers a number of important animal and human pathogens known to cause melioidosis ( B. pseudomallei ) and pulmonary infection in cystic fibrosis (CF) patients ( B. cepacia ), whereas B. thailandensis , which is closely related to B. pseudomallei , rarely gives rise to diseases in humans [29,30]. Both species of B. thailandensis and B. mallei display large chromosomal deletions when compared to B. pseudomallei .…”

Section: Resultsmentioning

confidence: 99%

GeneWiz browser: An Interactive Tool for Visualizing Sequenced Chromosomes

Hallin

Stærfeldt

Rotenberg

et al. 2009

Stand. Genomic Sci.

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We present an interactive web application for visualizing genomic data of prokaryotic chromosomes. The tool (GeneWiz browser) allows users to carry out various analyses such as mapping alignments of homologous genes to other genomes, mapping of short sequencing reads to a reference chromosome, and calculating DNA properties such as curvature or stacking energy along the chromosome. The GeneWiz browser produces an interactive graphic that enables zooming from a global scale down to single nucleotides, without changing the size of the plot. Its ability to disproportionally zoom provides optimal readability and increased functionality compared to other browsers. The tool allows the user to select the display of various genomic features, color setting and data ranges. Custom numerical data can be added to the plot allowing, for example, visualization of gene expression and regulation data. Further, standard atlases are pre-generated for all prokaryotic genomes available in GenBank, providing a fast overview of all available genomes, including recently deposited genome sequences. The tool is available online from http://www.cbs.dtu.dk/services/gwBrowser. Supplemental material including interactive atlases is available online at http://www.cbs.dtu.dk/services/gwBrowser/suppl/.

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Note: Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species

Cited by 408 publications

References 6 publications

Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis

Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis

Distribution of type III secretion gene clusters in Burkholderia pseudomallei, B. thailandensis and B. mallei

GeneWiz browser: An Interactive Tool for Visualizing Sequenced Chromosomes

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