Summary Cytogenetic studies were carried out on four species of Indian snakes viz Eryx johni (Family Boidae), Ptyas mucosus, Natrix stolata (Family Colubridae) and Bungarus caeruleus (Family Elapidae) collected with the help of handnet from Jammu region during the months May to Sept. 2004. Tissues, such as bone marrow and spleen were extracted and slides were prepared by air drying technique. Both Eryx johni and Ptyas mucosus exhibit 2nϭ34 with undifferentiated sex chromosomes which points to more closeness of Ptyas mucosus to Boidae than to other members of Colubridae. The diploid complement of Natrix stolata has 36 chromosomes (2nϭ36) with well differentiated sex chromosomes, while Bungarus caeruleus exhibits 2nϭ44 with sex chromosomes (ZW) and having submetacentric 'Z' and largest telocentric 'W'. This deviation from modale number of Ophidian (2nϭ36) with largest 'W' points towards its highly advanced nature.