Notizen: Quantification and Localisation of SH-Groups in Human Blood Serum Proteins

Schauenstein, E.; Dachs, F.

doi:10.1515/znc-1978-9-1038

Cited by 9 publications

(2 citation statements)

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“…We have previously reported that human IgG contains definite and consistent numbers of free sulfhydryl (SH) groups and labile disulfide bonds (SS*) that are detectable by disulfide exchange reaction with 5,5'-dithio-2,2'-dinitrobenzoic acid (dithionitrobenzoate; DTNB) [1][2][3][4]. In vestigations with purified subclass proteins revealed IgGl to carry 1 SS*/mol, whereas 1 SH could be assigned to the IgG2 molecule [3].…”

Section: Introductionmentioning

confidence: 99%

A Decrease in Reactive Disulfide Bonds of Serum IgG Signals a Characteristic Change in the IgG Subclass Pattern of Rats Bearing Experimental Tumors

Weblacher¹,

Leitsberger²,

Tillian³

et al. 1993

Int Arch Allergy Immunol

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Previous studies have shown that human IgG1 contains a ‘reactive’ disulfide bridge (SS*), detectable by a 24-hour disulfide exchange reaction, and that the serum level of this IgG subclass is selectively diminished in patients with various malignant diseases. Here we present evidence that in rats IgG2b is the only subclass that carries one SS* per molecule. Furthermore, it is shown that rats inoculated with experimental tumor lines, i.e., the Yoshida hepatoma ascites tumor and the Walker 256 carcinosarcoma growing in ascites or as solid tumor, exhibit significantly decreased SS* per mole IgG which corresponds to a selective diminution of IgG2b. Although at later stages there is a quantitative correlation with the tumor burden, with the Walker tumor this effect becomes significant as early as 24 h after inoculation, i.e., well before exponential tumor growth and an absolute reduction of total IgG. Control animals injected intraperitoneally with either viable spleen cells or irradiated Walker 256 cells did not show comparable alterations in their IgG subclass profile. Thus, the selective defect of IgG2b requires the presence of viable and proliferating tumor cells. Possible mechanism(s) of tumor-associated shifts in IgG subclasses are discussed.

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Section: Introductionmentioning

confidence: 99%

A Decrease in Reactive Disulfide Bonds of Serum IgG Signals a Characteristic Change in the IgG Subclass Pattern of Rats Bearing Experimental Tumors

Weblacher¹,

Leitsberger²,

Tillian³

et al. 1993

Int Arch Allergy Immunol

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“…In earlier communications [1][2][3] we studied the in teraction of human serum IgG with 5,5'-dithio(2,2'-dinitro)benzoic acid (DTNB) for determination of both free SH groups and labile S-S bonds. Using this method, consistent values were found in IgG from healthy persons for free SH groups (0.24 ± 0.02/mol IgG) detectable after 30 min reaction with DTNB.…”

Section: Introductionmentioning

confidence: 99%

Labile Disulfide Bonds and Free Thiol Groups in Human IgG II.

Schauenstein¹,

Reiter²,

Gombotz

et al. 1986

Int Arch Allergy Immunol

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As recently shown, the measurement of labile disulfide bonds and free thiol groups of human IgG using 5,5′-dithio(2,2′-dinitro)benzoic acid (DTNB) provides direct information about the relative concentrations of IgG₁ and IgG₂, respectively. Furthermore, using this technique data have been obtained that show a decrease in labile S-S (corresponding to IgG₁) and increase of free SH (corresponding to IgG₂) in IgG from patients afflicted with various malignant diseases. In the present study a total of 280 sera from patients with malignancies of various tissue origin was investigated. Significantly reduced ΣS values, i.e. the sum of free SH and labile S-S, were obtained with IgG from cases of carcinoma of the mammary gland, urinary bladder, skin and prostate, as compared to cases with benign diseases of the respective organs. Most pronounced differences were observed between benign and malignant disorders of the prostate. It was confirmed with this group of patients that the reduction of ΣS was due to the decrease of S-S bonds, whereas the number of free SH groups remained unchanged or was increased. The cases of mammary gland carcinoma were grouped according to clinical stage. A clear correlation between reduction of ΣS and tumor size was observed. The possible theoretical and clinical implications of these results are discussed.

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