Novel 3D biomaterials for tissue engineering based on collagen and macroporous ceramics

Abstract

In this study, different collagen and ceramic based materials were characterized concerning their mechanical and biocompatible properties. Therefore, they were tested in static and dynamic cultivation using human mesenchymal stem cells derived from adipose tissue.Zirconia ) and a porous collagen-elastin-matrix (Matriderm J ) were similarly evaluated concerning their maximal tension and maximal deformation and compared with decellularized skin. The maximal deformations of the porous collagen materials were comparable but significantly increased compared to decellularized skin. The maximal tension of the porous matrices was five times lower than the maximal tension of decellularized skin.For the evaluation for cell culture applications, Sponceram In a rotating bed bioreactor system (Z J RP system, Zellwerk GmbH) the human mesenchymal stem cells were cultured under dynamic conditions on Sponceram J over a time period of 47 days using osteogenic differentiation medium. Continuous increasing glucose consumption and lactate production gives evidence of good cell proliferation. After cultivation, the Sponceram J discs were stained with von kossa and alizarin red which showed matrix calcification.In summary, the tested collagen-and ceramic materials are applicable for the cell culture applications whereas the ceramic materials are presenting a promising approach for bone tissue engineering implementations.

Recellularization of auricular cartilage via elastase-generated channels

“…To estimate scaffold contraction, we compared the size of untreated auricular cartilage scaffolds to elastase-treated scaffolds recellularized with BMSCs or culture expanded ACs and then cultured in chondrogenic differentiation medium. As a reference for cell contraction, we seeded the same number of cells from the same donors onto an equally-sized collagen mesh (Matristypt™) used for tissue engineering [36]. The auricular cartilage scaffolds retained their shape and size after elastase treatment and during chondrogenic differentiation of BMSC or ACs, while the collagen mesh shrank severely ( figure 4(a)).…”

Technological Processes for Obtaining Monolithic Polydisperse Protein-Base Systems

“…The main processing industry concern in handling primary natural resource, which includes biological polymers of versatile groups, is attribution of homogeneity, as well as elimination of various noncharacteristic inclusions within them; dispergation, and ultimately, increase of the end-user performance of the product, including protein composites, 3D biomaterials, coatings, etc. [22].…”

Refractory Metal Coated Alumina Foams as Support Material for Stem Cell and Fibroblasts Cultivation

“…Cellular alumina supports have been used that almost perfectly represent trabecular bone-like artificial structures. However, due to their brittleness, ceramic cellular scaffolds may emit particles when used in biomedical applications [ 16 ]. For better cell and tissue compatibility, the macro-porous alumina supports were metallized with thin layers of bioactive materials such as Pt, Ti and Ta.…”