Novel 3D-Printed Device for the Simple Preparation of Hydrogel Beads

Rahman, Kazi Anisur; Ara, SathiGulsan; TaketaHiroaki,; Farahat, Mahmoud; OkadaMasahiro,; ToriiYasuhiro,; MatsumotoTakuya,

doi:10.1089/3dp.2014.0023

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…Microfluidic approaches for making ECM or hydrogel droplets are commonly generated and have been recognized as powerful tools for examining single cells (Joensson and Svahn 2012). Cells have been embedded in droplets of alginate (Chan et al 2016;Lin et al 2007;Park et al 2009), agarose (Rahman et al 2015), collagen (Hong et al 2012;Huang et al 2014;Jang et al 2017;Ma et al 2013;Patel et al 2015) and composite matrices (Yoshida et al 2017) or have been absorbed onto the outside of gelled collagen droplets (Yamada et al 2015;.…”

Section: Introductionmentioning

confidence: 99%

Microtissue size and cell-cell communication modulate cell migration in arrayed 3D collagen gels

Nuhn

Gong

Che

et al. 2018

Biomed Microdevices

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Cells communicate through the extracellular matrix (ECM) in many physiological and pathological processes. This is particularly important during cell migration, where cell communication can alter both the speed and the direction of migration. However, most cell culture systems operate with large volumes relative to cell numbers, creating low cell densities and diluting factors that mediate cell communication. Furthermore, they lack the ability to isolate single cells or small groups of cells. Droplet forming devices allow for an ability to embed single or small groups of cells into small volume segregated 3D environments, increasing the cell density to physiological levels. In this paper we show a microfluidic droplet device for fabricating 3D collagen-based microtissues to study breast cancer cell motility. MDA-MB-231 cells fail to spread and divide in small, thin chambers. Cell migration is also stunted as compared to thick 3D gels. However, larger chambers formed by a thicker devices promote cell spreading, cell division and faster migration. In the large devices, both cell-ECM and cell-cell interactions affect cell motility. Increasing collagen density decreases cell migration and increasing the number of cells per chamber increases cell migration speed. Furthermore, cells appear to sense both the ECM-chamber wall interface as well as other cells. Cells migrate towards the ECM-chamber interface if within roughly 150 μm, whereas cells further than 150 μm tend to move towards the center of the chamber. Finally, while cells do not show enhanced movement towards the center of mass of a cell cluster, their migration speed is more variable when further away from the cell cluster center of mass. These results show that microfluidic droplet devices can array 3D collagen gels and promote cell spreading, division and migration similar to what is seen in thick 3D collagen gels. Furthermore, they can provide a new avenue to study cell migration and cell-cell communication at physiologically relevant cell densities.

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Section: Introductionmentioning

confidence: 99%

Microtissue size and cell-cell communication modulate cell migration in arrayed 3D collagen gels

Nuhn

Gong

Che

et al. 2018

Biomed Microdevices

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“…The optimization of 3D inkjet printing techniques for alginate bioinks has facilitated the creation of structures that closely mimic tissue, supporting physiological flows [10]. Additionally, the introduction of hydrogel-based technologies has marked a significant stride in cell therapy and tissue engineering applications, offering precise size control for cell therapy and tissue engineering applications [44] and optimizing cell-friendly fabrication for physiological models [9]. This is complemented by developments in bone regeneration, where MXene composite hydrogels have demonstrated synergistic antibacterial and osteogenic effects [45], and in drug delivery systems, where thermosensitive hydrogels show potential for controlled drug release [13].…”

Section: Hydrogel Bioprinting Techniquesmentioning

confidence: 99%

“…Material science has played a crucial role in these advancements, with the exploration of hydrogel printability [49], rapid hydrogel bead fabrication [44], and the engineering of perfusable vasculature networks on-chip [9] being notable examples. These efforts not only enhance the practical applications of 3D printing technologies but also contribute to the broader understanding of material interactions and fabrication processes.…”

Section: Hydrogel Bioprinting Techniquesmentioning

confidence: 99%

“…Another trend is the focus on biocompatibility and cellular interactions, with numerous studies [9,13,44,45,54,55,58,[83][84][85] investigating cell viability, proliferation, differentiation, and specific biological responses. These assessments are vital for hydrogels intended for regenerative medicine, drug delivery systems, and tissue engineering, where the material's compatibility with biological systems is a key determinant of success.…”

Section: Hydrogel Testing and Evaluationmentioning

confidence: 99%

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Three-Dimensional Printing Strategies for Enhanced Hydrogel Applications

Omidian,

Mfoafo

2024

Gels

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This study explores the dynamic field of 3D-printed hydrogels, emphasizing advancements and challenges in customization, fabrication, and functionalization for applications in biomedical engineering, soft robotics, and tissue engineering. It delves into the significance of tailored biomedical scaffolds for tissue regeneration, the enhancement in bioinks for realistic tissue replication, and the development of bioinspired actuators. Additionally, this paper addresses fabrication issues in soft robotics, aiming to mimic biological structures through high-resolution, multimaterial printing. In tissue engineering, it highlights efforts to create environments conducive to cell migration and functional tissue development. This research also extends to drug delivery systems, focusing on controlled release and biocompatibility, and examines the integration of hydrogels with electronic components for bioelectronic applications. The interdisciplinary nature of these efforts highlights a commitment to overcoming material limitations and optimizing fabrication techniques to realize the full potential of 3D-printed hydrogels in improving health and well-being.

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Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro

Taketa

Sathi

Farahat

et al. 2015

Sci Rep

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In vitro fabricated biological tissue would be a valuable tool to screen newly synthesized drugs or understand the tissue development process. Several studies have attempted to fabricate biological tissue in vitro. However, controlling the growth and morphology of the fabricated tissue remains a challenge. Therefore, new techniques are required to modulate tissue growth. RGD (arginine-glycine-aspartic acid), which is an integrin-binding domain of fibronectin, has been found to enhance cell adhesion and survival; it has been used to modify substrates for in vitro cell culture studies or used as tissue engineering scaffolds. In addition, this study shows novel functions of the RGD peptide, which enhances tissue growth and modulates tissue morphology in vitro. When an isolated submandibular gland (SMG) was cultured on an RGD-modified alginate hydrogel sheet, SMG growth including bud expansion and cleft formation was dramatically enhanced. Furthermore, we prepared small RGD-modified alginate beads and placed them on the growing SMG tissue. These RGD-modified beads successfully induced cleft formation at the bead position, guiding the desired SMG morphology. Thus, this RGD-modified material might be a promising tool to modulate tissue growth and morphology in vitro for biological tissue fabrication.

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Novel 3D-Printed Device for the Simple Preparation of Hydrogel Beads

Cited by 4 publications

References 39 publications

Microtissue size and cell-cell communication modulate cell migration in arrayed 3D collagen gels

Microtissue size and cell-cell communication modulate cell migration in arrayed 3D collagen gels

Three-Dimensional Printing Strategies for Enhanced Hydrogel Applications

Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro

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