Novel ACE inhibitory tripeptides from ovotransferrin using bioinformatics and peptidomics approaches

Abstract

Food-derived ACE inhibitory peptides have recently attracted increased attention. This work focused on a more efficient in silico method to find ACE inhibitory peptides from ovotransferrin. In this work, ovotransferrin was digested into peptides by virtual enzymolysis. Subsequently, in vitro ACE inhibitory activity of potential tripeptides was conducted following the peptide score, toxicity, and water solubility prediction. Both pharmacophore study and flexible docking were applied to analyze ACE inhibition mechanism of tripeptides. Our results demonstrated that EWL was a potent ACE inhibitory tripeptide with IC50 value of 380 ± 10 μM. Besides, pharmacophore and flexible docking showed that the pi interaction and hydrogen bond were the key interactions in ACE-EWL complex. It appears that the in vitro ACE inhibitory activity of tripeptide EWL was consistent with its molecular modeling.

Enzymatic Hydrolysis of Ovotransferrin and the Functional Properties of Its Hydrolysates

“…Recently, Yu et al, (2019) exhibited potent ACE-inhibitory of ovotransferrin hydrolysates of 19 enzymes and one combination obtained from in silico hydrolysis. In addition, the peptides of ovotransferrin possess various immunomodulatory activities (Liu et al, 2017;Jiao et al, 2019), anti-inflammatory (Wang et al, 2017), and cytotoxic (Yi et al, 2017) activities.…”

“… Lee et al (2006) found that one peptide from ovotransferrin with the peptide sequence of Lys-Val-Arg-Glu-Gly-Thr-Thr-Tyr has an ACE-inhibitory activity that can be used as a pro-drug to reduce blood pressure. Recently, Yu et al. (2019) exhibited potent ACE-inhibitory of ovotransferrin hydrolysates of 19 enzymes and one combination obtained from in silico hydrolysis.…”

Functional properties of ovotransferrin from chicken egg white and its derived peptides: a review

“…Majumder et al ( 2015 ) reported that the ovotransferrin-derived peptide Ile-Arg-Trp increased the ACE-inhibitory activity but decreased the expression of proinflammatory gene in mesenteric arteries of spontaneously hypertensive rats. Recently, Yu et al ( 2019 ) reported that Glu-Trp-Leu obtained from in silico hydrolysis of ovotransferrin using 19 enzymes and one enzyme combination showed potent ACE-inhibitory activity. Moon et al ( 2017 ) reported that the promod 278P hydrolysate of ovotransferrin had 76.82 ± 1.28% ACE-inhibitory activity at 10 mg/mL concentration.…”

Stability and angiotensin converting enzyme inhibitory activity of peptide RVPSL-loaded graphene oxide

“…ACE-inhibitory activity was measured followed by high-performance liquid chromatography following a method reported previously (Yu et al, 2019).…”